Effect of Growth Rate on the Production of L-Proline in the Fed-batch Culture of Corynebacterium acetoacidophilum

  • 발행 : 2004.07.01

초록

Corynebacterium acetoacidophilum RYU3161 was cultivated in a L-histidine-limited fed-batch culture. To investigate the effect of cell growth on the L-proline production, 5 L fed-batch culture was performed using an exponential feeding rate to obtain the specific growth rates $(\mu)$ of 0.04, 0.06, 0.08, and 0.1 $h_1$. The results show that the highest production of L-proline was obtained at $\mu$ = 0.04 $h_1$. The specific L-proline production rate $(Q_p)$ increased pro-portionally as a function of the specific growth rate, but decreased after it revealed the maxi-mum value at $\mu$ = 0.08 $h_1$. Thus, the highest productivity of L-proline was 1.66 g $L^-^1 h^-^1$ at $\mu$ = 0.08 $h_1$. The results show that the production of L-proline in C. acetoacidophilum RYU3161 has mixed growth-associated characteristics.

키워드

참고문헌

  1. Amino Acid Products and Technology, Business Opportunity Report C-056 Ruffio, P.
  2. J. Am. Chem. Soc. v.74 Proline biosynthesis Vogel, H. J.;B. D. Davis https://doi.org/10.1021/ja01121a025
  3. Biochm. Biophys. Res. Commun. v.27 Glutamte kinase activity in Brevibac-terium flavum: relationship between L-proline and L-glutamine biosynthesis Yoshinaga, F.;Y. Takeda;S. Okumura https://doi.org/10.1016/S0006-291X(67)80053-6
  4. J. Bacteriol. v.178 Mutations in the Corynebacterium glutamicum Proline biosynthetic pathway: a natural bypass of the proA step Ankri, S.;L. Serebrijski;O. Reyes;G. Leblon https://doi.org/10.1128/jb.178.15.4412-4419.1996
  5. J. Gen. Appl. Microbiol. v.12 Studies on the fermentative production of L-proline, I. Production of L-proline by an isolecucine auxotrphic mutant of Brevibacterium flavum Yoshinaga, F.;S. Konishi;S. Okumura;N. Katsuya https://doi.org/10.2323/jgam.12.219
  6. Agr. Biol. Chem. v.39 Fermentation production of L-proline with auxotrophs of Corynebacterium glutamicum Araki, K.;L. Chibita;K. Nakayama;K. Takinami;H. Yamada
  7. J. Biol. Chem. v.46 Fermentation production of L-proline by DL-3,4- dehydo proline resistant mutant of L-glutamate producing bacteria Nakamori, S.;H. Morioka;F. Yoshingaga;S. Yamanaka
  8. J. Microbiol. Biotechnol. v.9 Isolation of a high-yield mutant strain for L-proline production and its fermentation conditions Ryu, W. S.;H. W. Jang;K. H. Cho;S. J. Chang;Y. W. Ryu;Y. H. Park
  9. Biotechnol. Bioprocess Eng. v.8 Production of recombinant hirudin in galactokinase-deficient Saccharomyces cerevisiae by fed-batch fermentation with continuous glucose feeding Ramisetti, S.;H. A. Kang;C. H. Kim https://doi.org/10.1007/BF02935894
  10. J. Biol. Chem. v.199 Photometric estimation of proline and ornithine Chinard, F. P.
  11. J. Ferment. Bioeng. v.83 Productivity improvement of a recombinant fermentation through decrease of growth rate differentials Kim, J. Y.;D. D. Y. Ryu https://doi.org/10.1016/S0922-338X(97)83577-5
  12. Biotechnol. Prog. v.14 Improvement of heterologous protein productivity by controlling postinduction specific growth rate in recombinant Escherichia coli under control of the $P_L$ promoter Lim, H. K.;J. H. Jung https://doi.org/10.1021/bp980059y
  13. Appl. Microbiol. Biotechnol. v.49 Growth-associated synthesis of recombinant human glucagon and human growth hormone in high-cell-density cultures of Escherichia coli Sin, C. S.;M. S. Hong;D. Y. Kim;H. C. Shin;J. Lee https://doi.org/10.1007/s002530051183
  14. Biotechnol. Bioprocess Eng. v.7 Kinetic analysis of the effect of cell density on hybridoma cell growth in batch culture Lee, Y. E. https://doi.org/10.1007/BF02935890
  15. Biotechnol. Bioeng. v.39 Metabolic characterization of L-lysine-producing strain by continuous culture Kiss, R. D.;G. Stephanopoulus
  16. Biotechnol. Prog. v.13 Substrate controlled fed-batch production of L-lysine with Corynebacterium glutamicum Weuster-Botz;D. R. Kelle;M. Frantzen;C. Wandrey https://doi.org/10.1021/bp970034j