Archives of Pharmacal Research

The Pharmaceutical Society of Korea (대한약학회)
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Cloning and Characterization of Directly Amplified Antiviral Gene Interferon Alpha-2b (HulFN$\{alpha}$-2b) from Human Leukocytes Chromosomal DNA

  • Behravan, Javad (Pharmaceutical Biotechnology Research Center, Mashhad University of Medical Sciences) ;
  • Ahmadpour, Hassan (Biotechnology section, School of Pharmacy, Mashhad University of Medical Sciences)
  • Published : 2004.07.01
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Abstract

Interferons are cytokines that confer resistance to viral infection and inhibit cellular proliferation. The interferon alpha gene from human blood samples was amplified, cloned and expressed in E. coli (BL21). Leukocyte chromosomal DNA was used as a source of template DNA. Using specific primers, the gene for HulFN$\{alpha}$-2b was amplified and inserted into the E. coli vector, pET21b, by ligation of the HindIII and BamHI linkers of the vector and insert. The insert was further analyzed by PCR, DNA restriction mapping and sequencing, and expressed in a suitable E. coli strain. The production of this important cellular protein in the laboratory has significant applications in production of the recombinant pharmaceutical proteins.

Keywords

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