Archives of Pharmacal Research

The Pharmaceutical Society of Korea (대한약학회)
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Cytotoxic Constituents of Diadema setosum

  • Minh, Chau-Van (Institute of Natural Products Chemistry, Vietnamese Academy of Science and Technology) ;
  • Kiem, Phan-Van (Institute of Natural Products Chemistry, Vietnamese Academy of Science and Technolog) ;
  • Huong, Le-Mai (Institute of Natural Products Chemistry, Vietnamese Academy of Science and Technolog) ;
  • Kim, Young-Ho (College of Pharmacy, Chungnam National University)
  • Published : 2004.07.01
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Abstract

5,8-Epidioxycholest-6-en-3-ol (1), cholesterol (2), glycerol 1-palmitate (3) and glycerol 1,3-dioleate-2-stearate (4) were isolated from the methanol extract of the sea urchin Diadema setosum, which was collected from the Halong sea, Vietnam. Chemical structures were established based on extensive 1D, 2D-NMR, FAB-MS, EI-MS spectroscopic data and GC-MS analysis. The NMR spectral data of compound 1 were reassigned by using HMQC and HMBC. Compound 1 was found to have strong cytotoxic effect against various cancer cell lines, such as KB ($IC_{50}$, 2.0 $\mu\textrm{g}$/mL), FL($IC_{50}$, 3.93 $\mu\textrm{g}$/mL), and Hep-2 ($IC_{50}$, 2.4 $\mu\textrm{g}$/mL) by in vitro assay.

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References

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