Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Polymorphism Analysis of the ND-4 Gene for the Origin Determination of Olive Flounder, Paralichthys olivaceus.

넙치의 원산지 판별을 위한 ND-4유전자의 다양성 분석

  • 송인선 (강릉대학교 동해안생명과학연구소) ;
  • 진덕희 (강릉대학교 생명대학 해양생명공학) ;
  • 최석정 (강릉대학교 자연대학 화학) ;
  • 이석근 (강릉대학교 치과대학 구강병리과)
  • Published : 2004.08.01
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Abstract

In order to distinguish the genetic polymorphism among the olive flounder (Paralichthys olivaceus) obtained from East sea of Jumunjin, aquaculture of Tongyoung and Geoje, and East sea of North Korea, the ND-4 and cytochrome b genes of olive flounder were divided into 5 regions. Each region was analyzed by degenerating gel electrophoresis scanning (DGES), and by subsequent DNA sequencing. The DGES disclosed characteristic DNA polymorphisms in ND-4-2 and ND-4-3 regions of olive flounder, which were also confirmed by the DNA sequencing. The olive flounders obtained from the different marine areas showed DNA mutations in ND-4-2 region (G390A, C402T, and A411G； GenBank： AB028664), and also showed frequent DNA mutations in ND-4-3 region (C515G, C537T, C538T, A567G, G714A, C736T, G756A, A759T, T817C, and T829G), white the cytochrome b gene showed no DNA mutation both in the DGES and DNA sequencing. These data suggest that the ND-4-2 and ND-4-3 regions are candidate loci to distinguish the origin of olive flounder, and that the DGES used in this study provided fast and reliable informations for the genetic polymorphism.

주문진 근해의 동해산 넙치, 통영과 거제의 양식산 넙치, 그리고 북한 해역의 동해산 넙치를 이용하여 넙치 ND-4와 cytochrome b유전자의 다양성을 관찰하기 위하여 DGES와 DNA 염기서열 검색을 병행하였는데, 각각의 다른 지역에서 얻은 넙치들은 ND-4-2와 ND-4-3 영역에서 특징적인 DNA 다양성이 있었으나 넙치의 cytochrome b유전자에서는 지역간의 차이를 보이는 유전자 변이가 발견되지 않았다. 따라서 본 연구에서 넙치의 지역별 차이를 구별하는 원산지 판별에는 사용된 DGES와 DNA 염기서열 검색 방법이 효과적이었으며, 넙치의 유전자에서는 개체간의 변이가 ND-4-2와 ND-4-3 영역에서 구별되는 유전자 다양성이 관찰되었으므로, 넙치의 원산지 판정을 위한 유전자 검사에는 ND-4-2와 ND-4-3영역의 검색이 필요하다.

Keywords

References

  1. Dollhopf, S. L., S. A. Hashsham and J. M. Tiedje. 2001. Interpreting 16S rDNA T-RFLP data: application of self- organizing maps and principal component analysis to describe community dynamics and convergence. Microb. Ecol. 42(4), 495-505 https://doi.org/10.1007/s00248-001-0027-7
  2. Gandrille, S., M. Goossens and M. Aiach. 1994. Scanning method to establish the molecular basis of protein C. deficiencies. Hum. Mutat. 4(1), 20-30 https://doi.org/10.1002/humu.1380040104
  3. Gao, Y. Q., M. Danciger, N. B. Akhmedov, D. Y. Zhao, J. R. Heckenlively, G. A. Fishman, R. G. Weleber, S. G. Jacobson and D. B. Farber. 1998. Exon screening of the genes encoding the beta- and gamma-subunits of cone transducin in patients with inherited retinal disease. Mol. Vis. 4, 16-20
  4. Garbeva, P., L. S. Overbeek, J. W. Vuurde and J. D. Elsas. 2001. Analysis of edophytic bcterial cmmunities of poato by plting and denaturing gradient gel electrophoresis (DGGE) of 16S rDNA based PCR fragments. Microb. Ecol. 41(4), 369-383 https://doi.org/10.1007/s002480000096
  5. Ge, W., M. E. Davis, H. C. Hines and K. M. Irvin. 1999. Polymorphism in exon 10 of the bovine GHR gene detected by PCR-DGGE. Anim. Genet. 30(2), 167-168 https://doi.org/10.1046/j.1365-2052.1999.00382-9.x
  6. Gelfi, C., S. C. Righetti, F. Zunino, G. Della Torre, M. A. Pierotti and P. G. Righetti. 1997. Detection of p53 point mutations by double-gradient, denaturing gradient gel electrophoresis. Electrophoresis 18(15), 2921-2927 https://doi.org/10.1002/elps.1150181533
  7. Ghanem, N., E. Girodon, M. Vidaud, J. Martin, P. Fanen, F. Plassa and M. Goossens. 1992. A comprehensive scanning method for rapid detection of beta-globin gene mutations and polymorphisms. Hum. Mutat. 1(3), 229-239 https://doi.org/10.1002/humu.1380010310
  8. Gottardi, E., M. Losekoot, R. Fodde, G. Saglio, C. Camaschella and L. F. Bernini. 1992. Rapid identification by denaturing gradient gel electrophoresis of mutations in the gamma-globin gene promoters in non-deletion type HPFH. Br. J. Haematol. 80(4), 533-538 https://doi.org/10.1111/j.1365-2141.1992.tb04569.x
  9. Ham, S. Y., S. K. Lee, H. S. Han and D. H. Jin. 2002. DNA heteropolymorphism of Chum Salmon detected by denaturing gradient gel electrophoresis and real time PCR. J. Korean Fish Soc. 35, 490-496 https://doi.org/10.5657/kfas.2002.35.5.490
  10. Hamelin, R., N. Jego, P. Laurent-Puig, M. Vidaud, and G. Thomas. 1993. Efficient screening of p53 mutations by denaturing gradient gel electrophoresis in colorectal tumors. Oncogene 8(8), 2213-2220
  11. Hanekamp, J. S., W. G. Thilly and M. A. Chaudhry. 1996. Screening for human mitochondrial DNA polymorphisms with denaturing gradient gel electrophoresis. Hum. Genet. 98(2), 243-245 https://doi.org/10.1007/s004390050201
  12. Kam, L. and T. Edward. 2001. Bacterial community dynamics in liquid swine manure during storage; molecular analysis using DGGE/PCR of 16S rDNA. FEMS Microbiol. Ecology 38, 169-177 https://doi.org/10.1111/j.1574-6941.2001.tb00895.x
  13. Mette, H. N. and B. R. Niels. 2002. Denaturing gradient gel electrophoresis (DGGE) approaches to study the diversity of ammonia-oxidizing bacteria. J. Microbiol. Methods 50, 189-203 https://doi.org/10.1016/S0167-7012(02)00026-X
  14. Nico B, D. W. Wim, V. Villy and M. T. Eva. 2002. Evaluation of nested PCR-DGGE (denaturing gradient gel electrophoresis) with group-specific 16S rRNA primers for the analysis of bacterial communities from different wastewater treatment plants. FEMS Microbiol. Ecology 39, 101- 112
  15. Popescu, T., M. Blazkova, L. Kozak, G. Jebeleanu, A. Popescu. 1998. Mutation spectrum and phenylalanine hydroxylase RFLP/VNTR background in 44 Romanian phenylketonuric alleles. Hum. Mutat. 12(5), 314-319 https://doi.org/10.1002/(SICI)1098-1004(1998)12:5<314::AID-HUMU4>3.0.CO;2-D
  16. Saiki, R. K., D. H. Gelfand, S. Stoffel, S. J. Scharf, R. Higuchi, G. T. Horn, K. B. Mullis and H. A. Erlich. 1988. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239(4839), 487-491 https://doi.org/10.1126/science.2448875
  17. Theelen, B., M. Silvestri, E. Gueho, A. van Belkum and T. Boekhout. 2001. Identification and typing of Malassezia yeasts using amplified fragment length polymorphism (AFLP), random amplified polymorphic DNA (RAPD) and denaturing gradient gel electrophoresis (DGGE). FEM Yeast Res. 1(2), 79-86 https://doi.org/10.1111/j.1567-1364.2001.tb00018.x

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