Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Inhibitory Effect of Bacteriophage EPS-Depolymerase on Growth of Asian Pear Blight Pathogen Erwinia pyrifoliae

  • Kim, Il-Gi (Department of Genetic Engineering, Hallym University) ;
  • Lee, Myung-Shin (Laboratory of Molecular Plant Pathology, College of Life and Environmental Sciences, Korea University) ;
  • Jin, Tae-Eun (Department of Genetic Engineering, Hallym University) ;
  • Hwang, Byung-Kook (Laboratory of Molecular Plant Pathology, College of Life and Environmental Sciences, Korea University) ;
  • Lee, Jin-Hyung (Department of Genetic Engineering, Hallym University) ;
  • Suh, Suk-Chul (National Institute of Agricultural Biotechnology, Rural Development Administration) ;
  • Rhim, Seong-Lyul (Department of Genetic Engineering, Hallym University)
  • Published : 2004.08.01
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Abstract

The plant pathogen Erwinia pyrifoliae was infected with bacteriophage PEa1(h), which produced a translucent halo plaque when grown on a lawn of E. pyrifoliae. To investigate the function of an exopolysaccharide (EPS)-depolymerase in the growth of E. pyrifoliae, an EPS-depolymerase gene was synthesized using the PCR method and sequenced. The synthesized gene was then transferred to E. pyrifoliae. The transformed E. pyrifoliae did not produce any ooze, and its growth was inhibited. However, the EPS-depolymerase did not appear to induce cell death. Accordingly, the present results suggest that an EPS-depolymerase may be effective in inhibiting the cell growth or infection of the pathogen E. pyrifoliae.

Keywords

References

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