Journal of Microbiology and Biotechnology

  • 제14권4호
  • /
  • Pages.772-776
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  • 2004
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

Characterization and Identification of Bacillus thuringiensis subsp. tenebrionis SR6 and SR8

  • Kim, Il-Gi (Department of Genetic Engineering, Hallym University) ;
  • Lee, Jae-Wook (Department of Genetic Engineering, Hallym University) ;
  • Suh, Suk-Chul (National Institute of Agricultural Biotechnology, Rural Development Administration) ;
  • Rhim, Seong-Lyul (Department of Genetic Engineering, Hallym University)
  • 발행 : 2004.08.01
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초록

Physiological and molecular characteristics of Bacillus thuringiensis SR6 and SR8 were investigated, and phase contrast and electron microscopies revealed that a large rhomboidal crystal protein was present in the sporulating cells. SDS-PAGE and Western blot analyses showed that B. thuringiensis SR8 produced 70 kDa protein much more than other proteins, and that the 70 kDa protein could bind to the antibody of B. thuringiensis subsp. tenebrionis-crystal toxin protein, indicating that the crystal 70 kDa protein has an immunological homology with B. thuringiensis subsp. tenebrionis-crystal toxin protein. The DNA fragment of B. thuringiensis subsp. tenebrionis-toxin gene was detected in B. thuringiensis SR6 and SR8 by using PCR amplification analysis. Furthermore, the insect bioassay showed the insecticidal activity against Colorado potato beetle larvae. Based on the physiological and molecular similarities to B. thuringiensis subsp. tenebrionis, it is suggested that the B. thuringiensis SR6 and SR8 may be mutants of the B. thuringiensis subsp. tenebrionis strain overexpressing the crystal of 70 kDa toxin protein.

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참고문헌

  1. MIcrobiol. Rev. v.50 Bacillus thuringiensis and related insect pathogens Aronson. A. I.;W. Beckmam;P. Dunn
  2. Anal. Biochem. v.72 A rapid and sensitive method for quantitiation of microgram quantities of protein utilizing the principle of protein-dye binding Bradford, M. M. https://doi.org/10.1016/0003-2697(76)90527-3
  3. J. Microbiol. Biotechnol. v.12 Isolation and identification of photosynthetic bacterium useful for wastewater treatment Choi, H. P.;H. J. Kang;H. C. Seo;H. C. Sung
  4. J. Microbiol. Biotechnol. v.12 Reevaluation of the change of Leuconostoc species and Lactobacillus plantarum by PCR during kimchi fermentation Choi, J. Y.;M. K. Kim;J. H. Lee
  5. J. Mol. Biol. v.56 Fate of transforming DNA following uptake by competent Bacillus subtilis Dubnau, D.;R. D. Abenson https://doi.org/10.1016/0022-2836(71)90460-8
  6. Microbiol. Rev. v.53 Insecticidal crystal proteins of Bacillus thuringiensis Hoefte, H.;H. R. Whiteley
  7. J. Microbiol. Biotechnol. v.13 Molecular structure of PCR cloned PHA synthase genes of Pseudomonas putida KT2440 and its utilization for medium-chain length polyhydroxyalkanoate production Kim, T. K.;H. D. Shin;M. C. Seo;J. N. Lee;Y. H. Lee
  8. Nature v.227 Cleavage of structural proteins during the assembly of the hed of bacteriophage T4 Laemmli, U. K. https://doi.org/10.1038/227680a0
  9. J. Microbiol. Biotechnol. v.12 Effect of Enterococcus faecalis strain PL9003 on adherence and growth of Helicobacter pylori Nam, H. R.;M. S. Ha;E. J. Lee;Y. H. Lee
  10. Molecular Breeding v.1 Development of insect resistance in tomato plants expressing the ${\sigma}$-endotoxin gene of Bacillus thuringiensis subsp. tenebrionis Rhim, S. L.;H. W. Cho;B. D. Kim;W. Schnetter;K. Geider https://doi.org/10.1007/BF02277423
  11. Molecular Cloning: A Laboratory Manual(2nd Ed.) v.2 Sambrook,J.;E.F.Fritsch;T.Maniatis
  12. Annu. Rev. Microbiol. v.40 The molecular biology of parasporal crystal body formation in Bacillus thuringiensis Whiteley, H. R.;H. E. Schnepf https://doi.org/10.1146/annurev.mi.40.100186.003001