Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Lipase-Catalyzed Synthesis of Structured Lipids with Capric and Conjugated Linoleic Acid in a Stirred-Batch Type Reactor

대두유로부터 Lipase를 이용한 재구성 지질의 합성 및 특성

  • Published : 2004.08.01
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Abstract

Structured lipid (SL) was produced from soybean oil with molar ratio of 1：2：2 (soybean oil：capric acid：CLA) using Chirazyme L-2 lipase (4％ by weight of total substrates). The reaction was conducted for 24 hr at 55$^{\circ}C$ in a 1 L stirred-batch type reactor. SL-soybean oil contained 4.9 mol％ capric acid and 4.1 mol％ CLA, respectively. Iodine value of SL-soybean oil was reduced than that of soybean oil due to the incorporated capric acids. Tocopherol content in SL-soybean oil was 18.2 mg/l00 g. SL-soybean oil appeared more yellowish color than soybean oil. Reverse-phase HPLC showed that SL-triacylglycerol species containing capric acid consisted of about 12.6 area％.

Scale-up하기 위하여 24시간동안 1：2：2의 몰 비율로 stirred-batch type reactor에서 합성된 재구성지질은 capric acid와 CLA를 각각 4.9 mol%와 4.1 mot% 함유하였다. 기능성 지방산인 capric acid와 CLA는 주로 TAG의 sn-1,3 position에 분포하였고, sn-2 position에는 linoleic acid가 대부분을 차지하였다. 재구성지질과 대두유의 요오드가는 각각 126와 114이었고, 비누화가는 각각 190과 194로 측정됨으로써, 재구성 지질의 TAG에서 capric acid의 함유를 확인할 수 있었다. 대두유와 재구성지질의 tocopherol함량은 각각 74.6 mg/100 g과 18.2 mg/100 g으로, 재구성지질의 tocopherol 함량은 대두유보다 약 76% 감소하였다. 색도분석 결과, 재구성 지질과 대두유간에 백색도(L＊)와 적색도(a＊)에서는 유의적 차이가 없었으나(p＜0.05),황색도(b＊)에서 재구성지질이 대두유보다 좀더 yellowness를 보이며 유의적 차이를 보였다. (p＜0.05). Reversed-phase HPLC 분석 결과, 재구성지 질에서 capric acid를 함유하고 있는 TAG형태의 partition number (PN)는 25∼37로 약 12.57 area%이었음을 확인하였다. 대두유의 주된 crystallization peak는 -57.86$^{\circ}C$C이었으나, capric acid를 4.9 mol% 함유하고 있는 재구성지질의 crystallization peak는 -52.78$^{\circ}C$로 대두유보다 결정화가 좀더 고온에서 일어났다.

Keywords

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