Reproductive and Developmental Biology

  • 제28권2호
  • /
  • Pages.107-112
  • /
  • 2004
  • /
  • 1738-2432(pISSN)
  • /
  • 2288-0151(eISSN)
한국동물번식학회 (The Korean Society of Animal Reproduction)
권호 표지

Characterization of Quantitative Trait Loci (QTL) for Growth using Genome Scanning in Korean Native Pig

  • Lee, H.K. (Genomic Informatics Center, Hankyong National University) ;
  • Choi, I.S. (Department of Bionolgy Genomic Engineering, Hankyong National University) ;
  • Choi, B.H. (Genomic Informatics Center, Hankyong National University) ;
  • Kim, T.H. (Genomic Informatics Center, Hankyong National University) ;
  • Jung, I.J. (Genomic Informatics Center, Hankyong National University)
  • 발행 : 2004.06.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Molecular genetic markers were genotyped used to detect chromosomal regions which contain economically important traits such as growth traits in pigs. Three generation resource population was constructed from a cross between the Korean native boars and Landrace sows. A total of 193 F2 animals from intercross of F1 were produced. Phenotypic data on 7 traits, birth weight, body weight at 3, 5, 12, 30 weeks of age, live empty weight were collected for F2 animals. Animals including grandparents (F0), parents (F1), offspring (F2) were genotyped for 194 microsatellite markers covering from chromosome 1 to 18. Quantitative trait locus analyses were performed using interval mapping by regression under line-cross model. To characterize presence of imprinting, genetic full model in which dominance, additive and imprinting effect were included was fitted in this analysis. Significance thresholds were determined by permutation test. Using imprinting full model, four QTL with expression of imprinted effect were detected at 5% chromosome-wide significance level for growth traits on chromosome 1, 5, 7, 13, 14, and 16.

키워드

참고문헌

  1. Andersson, L., Haley, C.S., Ellegren, H., Knott, S.A. and Johansson, M. et al. 1994. Genetic mapping of quantitative trait loci for growth and fatness in pig. Science 263:1771-1774
  2. Churchill, G.A. and Doerge, R.W. 1994. Empirical threshold values for quantitative trait mapping. Genetics 138, 963-971
  3. Cockett, N.E., Jackson, S.P., Shay, T.L., Farnir, F., Berghmans, S., Snowder, G.D., Nielsen, D.M. and Georges, M. 1996. Polar overdominance at the ovine callipyge locus. Science 273(5272):236-8
  4. De Koning, D.J., Janss, L.L.G., Rattink, A.P., Van, Oers, P.A.M., de Vries, B,J., Groenen, M.A.M., Van Der Poel, J.J., DeGrott, P.N., Brascamp, E.W. and Van Arendonk, J.A.M. 1999. Detection of quantitative trait loci for backfat thickness and intramuscular fat content in pig (Susscrofa). Genetics 152:1679-1690
  5. De Koning, D,J., Rattink, A.P., Harlizius, B., Johan, A.M., Van Arendonk, J.A.M., Brascamp, E.W. and Groenen, M.A.M. 2000a. Genome-wide scan for body composition in pig reveals important role of imprinting. Proc. Natl. Acad. Sci. USA 97:7947-7950
  6. De Koning, D.J., Rattink, A.P., Harlizius, B., Van, Bovenhuis, H. and Groenen, M.A.M. et al. 2000b. Detection and characterization of quantitative trait loci for carcass and meat quality trait in pigs. Proc. Europ. Assoc. Anim. Prod. 6:3
  7. De Vries, A.G, Kerr, R., Tier, B. and Long, T. 1994. Garnetic imprinting effects on rate and composition of pig growth. Theor. Appl. Genet. 88:1037-1042
  8. Green, P., Fall, I. and Crook, S. 1990. Documentation for CRI-MAP version 2.4 (St. Louis Washington Uni-versity School of Medicine)
  9. Haley, C.S., Knott, S.A. and Elsen, J.M. 1994. Ma-pping quantitative trait loci in crosses between outbred lines using least squares. Genetics 136, 1195-1207
  10. Joen, J.T., Carlborg, O., Tornsten, A., Giuffra, E., Amarger, V., Chardon, P., Andersson, L., Andersson, K., Hansson, I., Lundstrom, K. and Andersson, L. 1999. A paternally expressed QTL affecting skeletal and cardiac muscle mass in pig mass to the IGF2 locus. Nature Genet. 21:157-158 https://doi.org/10.1038/5938
  11. Knott, S.A., Marklund, L., Haley, C.S., Andersson, K, Davies, W., Ellegren, H., Fredholm, M., Hansson, I., Hoyheim, B., Loudstrom, K., Moller, M. and An-dersson, L. 1998. Multiple marker mapping of quan-titative trait loci in a cross between outbred wild boar and large white pigs
  12. Maleck, M., Dekkers, J.C.M. and Lee, H. 2001. A molecular genome scan analysis to identify chromo-somal regions influencing economic traits in the pig. II. Meat and muscle composition. Mamm. Genome. submitted
  13. Nezer, C., Moreau, L., Brouwer, B., Coppieters, W., Detilleux, J., Hanset, R., Karim, L., Kvasz, A., Leroy, P. and George, M. 1999. An imprinted QTL with major effect on muscle mass and fat drposition maps to the IGF2 locus in pig. Nat. Genet. 21:155-157 https://doi.org/10.1038/5935
  14. Rothschild, M.F. and M. Soller. 1977. Candidate gene analysis to detect gene controlling traits of economic importance in domestic livestock. Probe 8:13-20
  15. Wada, Y., Akita, T., Awata, T., Furukawa, T., Sugai, N., Inage, Y., Ishii, K., Ito, Y., Kobayashi, E., Ku-sumoto, H., Matsumoto, T., Mikawa, S., Miyake, M., Murase, A., Shimanuki, S., Sugiyama, T., Uchida, Y., Yanai, S. and Yasue, H. 2000. Quantitative trait loci (QTL) analysis in a Meishan x Gottingen cross population. Anim. Genet. 31(6):376-84
  16. Wang, L., Yu, T.P. Tuggle, C.K. Liu, H.C. and Ro-thschild, M.F. 1998. A directed search for quantita-tive trait loci on chromosomes 4 and 7 in the pig. J. Anim. Sci. 76:2560-256715
  17. Wilkie, P.J., Paszek, A.A., Beattie, C.W., Alexander, L.J., Wheeler, M.B. and Schook, L.B. 1999.A genomic scan of porcine reproductive traits reveals possible quantitative trait loci (QTLs) for number of corpora lutea. Mamm Genome. 10(6):573-8 (Received: 19 May 2004 / Accepted: 18 June 2004)