Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
권호 표지

Effects of the Administration of Human Menopausal Gonadotropin on the Pregnancy in Rats

임신 랫드에 투여한 Human Menopausal Gonadotropin이 임신에 미치는 영향

  • Published : 2004.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

The effect of Human Menopausal Gonadotropin(hMG) on the implantation, pregnancy, and the concentration of plasma progesterone were studied in pregnant rats. HMG 75 or 150 IU were administered once on day 1, 2, 3, 4, 9, 12 or 14 of gestation, respectively. Rats were autopsied on day 7 or 18. A single dose of hMG prevented implantation and terminated pregnancy in all of the rats by injecting on either day 1 or day 2 and this abortifacient action was effective 82-98% of pregnant rats on day 3 or 4 and 14-20% on day 9 or 12. Administration of hMG had no effect on termination of pregnancy on day 14. Plasma progesterone concentration by injecting hMG on day 1, 2, 3 or 4 were very decreased.

Keywords

References

  1. Alcivar AA, Maurer RR, Anderson LL. Endocrine changes in beef heifers superovulated with follicle-stimulating hormone (FSH-P) or human menopausal gonadotropin. J Anim Sci 1992; 70: 224-31
  2. Arimura A, Pedroza E, Vilchez-Martinez J, Schally AV. Prevention of implantation by [D-Trp6] -LH-RH in the rat: comparative study with the effects of large doses of hCG on pregnancy. Endor Res Comrnun 1977; 4: 357 https://doi.org/10.3109/07435807709073628
  3. Banik UK. Pregnancy-terminating effect of human chorionic gonadotropin in rats. J Reprod Fert 1975; 42: 67-76 https://doi.org/10.1530/jrf.0.0420067
  4. Beattie CW, Corbin A, Cole G, Corry S, Jones RC, Koch K, Tracy J. Mechanism of the postcoital contraceptive effect of GnRH in the rat. 1. Serum hormone levels during chronic LHRH administration. BioI Reprod 1977; 16: 322-332 https://doi.org/10.1095/biolreprod16.3.322
  5. Bex FJ, Corbin A. Mechanism of the postcoital contraceptive effect of luteinizing hormone-releasing hormone: ovarian luteinizing hormone receptor interactions. Endocrinology 1979; 105: 139-145 https://doi.org/10.1210/endo-105-1-139
  6. Booth NH, McDonald LE. Veterinary pharmacology and therapeutics. 6th ed. Ames. The Iowa State University Press. 1988: 604-607
  7. Cochrane RL, Smalstig EB, Bennett DR, Shaar CJ Assessment of the progestogenic activity of compuinds by their effect on implantation in rats with diapausing embryos. J Reprod Fert Suppl 198l; 29: 187-190
  8. Cook AS, Webster BW, Keel BA. Variation in the biologic and biochemical characteristics of human menopausal gonadotropin. Fertil Steril 1988; 49: 704-12
  9. Corbin A, Beattie CW, Tracy J, Jones R, Foell TJ, Yardley J, Rees RWA. The anti-reproductive pharmacology of LH-RH and agonistic analogues. Int J Fertil 1978; 23: 81-92
  10. Corsan GH, Kemmann E. The role of superovulation with menotropins in ovulatory infertility: a review. Fertil Steril 1991; 55: 468-77
  11. Elbaum DJ, Bender EM, Brown JM, Keyes PL. Serum progesterone in pregnant rats with ectopic or in situ corpora lutea: Correlation between amount of luteal tissue and progesterone concentration. BioI Reprod 1975; 13: 541-545 https://doi.org/10.1095/biolreprod13.5.541
  12. Espey LL, Stacy S, Hayter H, Fujii S. Termination of early pregnancy in the rat by a single dose of human menopausal gonadotropin. Early Pregnancy: Biology and Medicine 1997; 3: 23-26
  13. Fujinaga M, Mazze RI. Reproductive and teratogenic effects of lidocaine in Spragu-Dawley rats. Anesthesiology 1986; 65: 626-636 https://doi.org/10.1097/00000542-198612000-00010
  14. Gemzell CA, Diczfalusy E, Tillinger KG. Clinical effect of human pituitary follicle stimulating hormone. J Clin Endocrinol Metab 1958; 18: 1333 https://doi.org/10.1210/jcem-18-12-1333
  15. Gidley-Baird AA. Endocrine control of implantation and delayed implantation in rats and mice. J Reprod Fert Suppl 1981; 29: 97-109
  16. Gougeon A, Testart J. Influence of human menopausal gonadotropin on the recruitment of human ovarian follicles. Fertil Steril 1990; 54: 848-52
  17. Grota LJ, Eik-Nes KB. Plasma progesterone concentrations during pregnancy and lactation in the rat. J Reprod Fert 1967; 13: 83-91 https://doi.org/10.1530/jrf.0.0130083
  18. Hahn DW, Demers LM, McGuire JL. Studies on the mechanism of the antifertility activity of human chorionic gonadotropin in rodents. Contraception 1980; 21: 551-560 https://doi.org/10.1016/0010-7824(80)90018-9
  19. Harlin J, Khan S, Diczfalusy E. Molecular composition of luteinizing hormone and follicle-stimulating hormone in commercial gonadotropin preparations. Fertil Steril 1986; 46: 1005-61
  20. Humphrey RR, Windsor BL, Reel JR, Edgren RA. The effects of luteinizing hormone releasing hormone (LH-RH) in pregnant rats. 1. Postnidatory effects. BioI Reprod 1977; 16: 614-621 https://doi.org/10.1095/biolreprod16.5.614
  21. Lauria A, Genazzani AR, Oliva O, Inaudi P, Crenonesi F, Monittola C, Aureli G. Clinical and endocrinological investigations on superovulation induced in heifers by human menopausal gonadotropin. J Reprod Fertil 1982; 66: 219-25 https://doi.org/10.1530/jrf.0.0660219
  22. Lunenfeld B, Menzi A, Volet B. Clinical effects of human menopausal gonadotropin. Acta Endocrinol (Copenh) 1960; 51: 587
  23. Morishige WK, Pepe GJ, Rothchild I. Serum luteinizing hormone, prolactin and progesterone levels during pregnancy in the rat. Endocrinology 1973; 92: 1527-1530 https://doi.org/10.1210/endo-92-5-1527
  24. NordenvaIl M, Ulmsten U, Ungerstedt U. Influence of progeterone on the sodium and potassium concentrations of rat uterine fluid investigated by Microdialysis. Gynecol Obstet Invest 1989; 28: 73-77 https://doi.org/10.1159/000293518
  25. Pepe GJ, Rothchild I. A comparative study of serum progesterone levels on pregnancy and in various types of pseudopregnancy in the rat. Endocrinology 1974; 95: 275-279 https://doi.org/10.1210/endo-95-1-275
  26. Ryoichi H, Hironori O, Takashi M, Masao I. Induction of superovulation and superpregnancy in adult Sprague-Dawley (SD) rats by human menopausal gonadotropin (hMG) injection. Nippon Funin Gakkai Zasshi 1999; 44: 233-238
  27. Ryoichi H, Masao I, Ruriko M, Eisei O. Induction of superovulation in rats using human menopausal gonadotropin (hMG). Nippon Funin Gakkai Zasshi 1990; 35: 433-7
  28. Salazar H, Furr BJA, Smith Gk, Bentley M, Gonzalez-Angulo A. Luteolytic effects of a prostaglandin analogue, clopostenol (lCI 80,996), in rats; ultrastructural and biochemical observations. Bio Reprod 1976; 14: 458-472 https://doi.org/10.1095/biolreprod14.4.458
  29. Stone BA, Quinn K, Quinn P, Vargyas JM, Marrs RP. Responses of patients to different lots of human menopausal gonadotropins during controlled ovarian hyperstimulation. Fertil Steril 1989; 52: 745-52
  30. Sugano M, Shinogi T, Nakada K. Nakao T. Endocrine profiles and embryo quality in Japanese black cattle superovulated with human menopausal gonadotropin and porcine follicle stimulating hormone. Reprod Domest Anim 2001; 36: 57-63 https://doi.org/10.1046/j.1439-0531.2001.00192.x
  31. Sugano M, Takano T, Saro N, Ohsaki J, Watanabe S. Effects of human menopausal gonadotropin (hMG) for superovulation in Japanese Black cattle. J Vet Med Sci 1995; 57: 1113-5 https://doi.org/10.1292/jvms.57.1113
  32. Taya K, Greenwald GS. In vivo and in vitro ovarian steroidogenesis in the pregnant rat. BioI Reprod 1981; 25: 683-691 https://doi.org/10.1095/biolreprod25.4.683
  33. Weisz J, Ward IL. Plasma testosterone and progesterone titers of pregnant rats, their male and female fetuses, and neonatal offspring. Endocrinology 1980; 106: 306-316 https://doi.org/10.1210/endo-106-1-306
  34. Wiest GW Progesterone and 20 $\alpha$ -hyroxypretn-4-en-3-one in plasma, ovaries and uteri during pregnancy in the rat. Endocrinology 1970; 87: 43-48 https://doi.org/10.1210/endo-87-1-43
  35. Willson CA, Horth CE, Endersby CA, McDonald PG. Changes in plasma levels of oestradiol, progesterone and luteinizing hormone in immature rats treated with pregnant mare serum gonadotrophin. J Endocr 1974; 60: 293-304 https://doi.org/10.1677/joe.0.0600293
  36. Yang WH, Chang MC. Interruption of pregnancy in the rat and hamster by administration of PMS or HCG. Endocrionology 1968; 83: 217-24 https://doi.org/10.1210/endo-83-2-217
  37. 김영홍, 장인호, 유창준, 이근우. 임신랫드에 임마혈청성성선자극호르몬을 투여했을 때 기형발생 또는 태아흡수에 미치는 영향. 한국수의공중보건학회지 1994; 18: 191-201
  38. 김영홍. 임신랫드에 투여한 HCG가 estrogen과 progesterone 및 Na와 K농도에 미치는 영향. 한국수의공중보건학회지 1996; 20: 97-106