KIEE International Transactions on Electrophysics and Applications

대한전기학회 (The Korean Institute of Electrical Engineers)
권호 표지

A Bio-Inspired Cell-Microsystem to Manipulate and Detect Living Cells

  • Lim, Jung-Min (School of Electrical and Computer Engineering, Seoul National University) ;
  • Byun, Sang-Won (School of Electrical and Computer Engineering, Seoul National University) ;
  • Park, Tai-Hyun (School of Chemical Engineering, Seoul National University) ;
  • Seo, Jong-Mo (Department of Ophthalmology, Seoul National University) ;
  • Yoo, Young-Suk (Department of Ophthalmology, Seoul National University) ;
  • Hum Chung (Department of Ophthalmology, Seoul National University) ;
  • Dong-il (School of Electrical and Computer Engineering, Seoul National University)
  • 발행 : 2004.08.01
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초록

In this study, we demonstrate for the first time a bio-inspired Cell-Microsystem to manipulate and detect living cells. Cultured retinal pigment epithelial cell line (ARPE-19) was directed to grow in a pre-defined Cell-Microsystem. The three-dimensional micropillars of 5 ${\mu}{\textrm}{m}$ in height and diameter of the Cell-Microsystem were fabricated. Inhibited DNA synthesis and transformed cell morphology were observed throughout the culture period. The demonstration of manipulating and detecting living cells by the surface topography is a new approach, and it will be very useful for the future design of cell-based biosensors and bioactuators.

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참고문헌

  1. Chen CS, Alonso JL, Ostuni E, Whitesides GM, Ingber DE. Cell shape provides global control of focal adhesion assembly. Biochem Biophys Res Commun 2003:307:355-6
  2. Takayama S, Ostuni E, LeDuc P, Naruse K, Ingber DE, Whitesides GM. Subcellular positioning of small molecules. Nature 2001. Jun 28;411(6841):1016
  3. Chiu DT, Jeon NL, Huang S, Kane RS, Wargo CJ, Chio IS, Ingber DE, Whitesides GM. Patterned deposition of cells and proteins onto surfaces by using three-dimensional microfluidic systems. Proc Natl Acad Sci USA 2000:97:2408-13
  4. Bhatia SN, Yarmush ML, Toner M. Controlling cell interactions by micropatterning in co-cultures: hepatocytes and 3T3 fibroblasts. J Biomed Mater Res 1997:34: 189-99
  5. van Kooten TG, von Recum AF. Cell adhesion to textured silicone surfaces: the influence of time of adhesion and texture on focal contact and fibronectin fibril formation. Tissue Eng 1999:5:223-40
  6. Molina T, Kabsch K, Alonso A, Kohl A, Komposch G, Tomakidi P. Topographic changes of focal adhesion components and modulation of p125FAK activation in stretched human periodontal ligament fibroblasts. J Dent Res 2001:80:1984-9
  7. Branch DW, Wheeler BC, Brewer GJ, Leckband DE. Long-term maintenance of patterns of hippocampal pyramidal cells on substrates of polyethylene glycol and microstamped polylysine. IEEE Trans Biomed Eng 2000:47:290-300
  8. Kwon K, Pak J, Park H, Lee KJ, Jin J, Ahn DJ. The patterning of Hippocampal neurons using PDMS microstamp. Proc. 4th Korean MEMS Conf., pp. 309313, Kyeongju, Korea, April 2002
  9. Turner AM, Dowell N, Turner SW, Kam L, Isaacson M, Turner JN, Craighead HG, Shain W. Attachment of astroglial cells to microfabricated pillar arrays of different geometries. J Biomed Mater Res 2000: 51: 430-41
  10. Lim JM, Chang JK, Cho DI. Microfabricated three dimensional PDMS interface modulate the expression of matrix metalloproteinase (MMP)-2 mRNA in cultured retinal pigment epithelial cells. The 14th Molecular electronics and devices symposium, p. 130, Seoul, Korea, March 2003
  11. Lim JM, Cho DI, Choi BD, Park TH, Chung S, Chang JK. Cell cycle progression and morphology of retinal pigment epithelial cells controlled by threedimensional micropatterned surfaces, Proceedings of Sensors Conference 2002, vol. 13, no. 1, pp. 119-122, Seoul, Korea, Nov. 2002
  12. Choi YS, Park DH, Kwon, YS, Kawai T. Development of New DNA Chip and Genome Detection Using an Indicator-free Target DNA. J KIEE 2003:52:365-370
  13. Cho SB, Hong JS, Kim YM, Pak JH. Electrochemical measurement for analysis of DNA sequence. J KIEE 2002:51:92-97
  14. Lee SK, Choi Y, An HJ, Pak JH, Sim WY, Yang SS. Fabrication of Microactuators Using Conductive Polymer. J KIEE 2000:49:698-704