KIEE International Transactions on Electrophysics and Applications

The Korean Institute of Electrical Engineers (대한전기학회)
권호 표지

Electrochemical Gene Detection Using Microelectrode Array on a DNA Chip

  • Park, Yong-Sung (School of Electrical, Electronic and Information Engineering, Wonkwang University) ;
  • Kwon, Young-Soo (Div. of Electrical, Electronic and Computer Eng., Dong-A University) ;
  • Park, Dae-Hee (School of Electrical, Electronic and Information Engineering, Wonkwang University)
  • Published : 2004.08.01
Download PDF
⟨ Previous Next ⟩

Abstract

In this study, a DNA chip with a microelectrode array was fabricated using microfabrication technology. Several probe DNAs consisting of mercaptohexyl moiety at their 5' end were immobilized on the gold electrodes by a DNA arrayer. Then target DNAs were hybridized and reacted with Hoechst 33258, which is a DNA minor groove binder and electrochemically active dye. Linear sweep voltammetry or cyclic voltammetry showed a difference between target DNA and control DNA in the anodic peak current values. It was derived from Hoechst 33258 and concentrated at the electrode surface through association with the formed hybrid. This suggested that this DNA chip could recognize the sequence specific genes.

Keywords

References

  1. S.P.A. Fodor, J.L. Read, M.C. Pirrung, L. Stryer, A.T. Lu, D. Salas, 'Light-directed, spatially addressable parallel chemical synthesis', Science, 51,pp.767773, 1991
  2. S.P.A. Fodor, R.P. Rava, X.C. Juang, A.C. Pease, C.P. Holmes, C.L. Adams, 'Multiplexed biochemical assays with biological chips', Nature, 364, pp.555556,1993
  3. R.F. Service, 'Microchip Arrays Put DNA on the Spot', Science, 282, 16, pp.396-399, 1998
  4. M. Schena, D. Shalon, R.W. Davis, P.O. Brown, 'Quantitative monitoring of gene expression patterns with a complementary DNA microarray', Science, 270, ppA67-470, 1995
  5. T. Livache, B. Fouque, A. Roget, J. Marchand, G. Bidan, R. Teoule, and G. Mathis, 'DNA Chip on a Silicon Device : Example of Hepatitis C Virus enotyping', Analytical Biochemistry, 255, pp.188194,1998
  6. D.Y. Guschin, B.K. Mobarry, D. Proudnikov, D.A. Stahl, B.E. Rittmann, and A.D. Mirzabekov, 'Oligonucleotide Microchips as Genosensors for Determinative and Environmental Studies in Microbiology', Appl. Environ. Microbiol., 63(6), pp.2397-2402, 1997
  7. E. Palecek, M. Fojta, M. Tomschik, J. Wang, 'Electrochemical biosensors for DNA hybridization and DNA damage', Biosens. & Bioelectron., 13, pp.621-628,1998
  8. D.H. Johnson, K.C. Glasgow, and H.H. Thorp, 'Electrochemical Measurement of the Solvent Accessibility of Nucleobases Using Electron Transfer between DNA and Metal Complexes', J. Am. Chem. Soc., 117, pp.8933-8938, 1995.
  9. P.A Ropp and H.H. Thorp, 'Site-selective electron transfer from purines to electrocatalysts voltammetric detection of a biologically relevant deletion in hybridized DNA duplexes', Chem. Biel., 6, pp.599-605, 1999.
  10. K.Hashimoto, K.Ito and Y. Ishimori, 'Novel DNA sensor for electrochemical gene detection', Anal. Chim. Acta, 286, p.219, 1994
  11. K. Hashimoto, K. Ito and Y. Ishimori, 'Sequencespecific Gene Detection with a Gold Electrode Modified with DNA Probes and an Electrochemically Active Dye', Anal. Chern., 66, p.3830, 1994
  12. F.G. Loontiens, L.W. Mclaughlin, S. Diekmann, R.M. Clegg, 'Binding of Hoechst 33258 and 4' ,6'diamidino- 2-phenylindole to self-complementary decadeoxy- nucleotides with modified exocyclic base substituents', Biochemistry, 30, p.182, 1991
  13. Y. S. Choi, D. K. Kim and Y. S. Kwon, 'Highly Integrated DNA Chip Microarrays by Hydrophobic Interaction', KIEE Inter. Trans. on EA, Vol. 11C, No. 2, pp. 23-27, 2001
  14. D. K. Kim, Y. S. Choi, Y. Murakami, E. Tamiya and Y. S. Kwon, 'Real-Time Detection of DNA Hybridization Assay by using Evanescent Field Microscopy', KIEE Inter. Trans. on EA, Vol. 11C, No.3, pp. 85-90, 2001
  15. Y. S. Choi, D. H. Park, Y. S. Kwon and T. Tomoji, 'Development of New DNA Chip and Genome Detection Using an Indicator-free Target DNA', Trans. KIEE, Vol. 52C, No.8, pp. 365-370, 2003
  16. Y. S. Choi, Y. S. Kwon, E. Tamiya, and D. H. Park, 'Three-Dimensional Self-Assembled Micro-Array Using Magnetic Force Interaction', KIEE Inter. Trans. on EA, Vol. 3-C, No.5, pp.182-188, 2003