Journal of Nutrition and Health

The Korean Nutrition Society (한국영양학회)
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Effect of Dietary Iron Levels on Lipid Metabolism, Antioxidative and Antithrombogenic Capacities in 16-month-old Rats

식이 철분 수준이 노령흰쥐의 지방대사, 항산화능 및 항혈전능에 미치는 영향

  • 김순기 (이화여자대학교 식품영양학과) ;
  • 박주연 (이화여자대학교 식품영양학) ;
  • 김미경 (이화여자대학교 식품영양학과)
  • Published : 2004.05.01
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Abstract

This study was conducted to examine the effect of dietary iron levels on lipid metabolism, antioxidative and antithrombogenic capacities in 16-month-old rats. Thirty-two Sprague-Dawley male 16-month-old rats weighing 618 $\pm$ 6 g were raised for 10 days with medium-iron diet (35 ppm in diet) and blocked into 4 groups according to their body weights. One of groups was sacrificed to obtain initial data and the rest 3 groups were raised for 3 months with experimental diets containing different levels of iron (5 ppm, 35 ppm, and 350 ppm). Total lipid, triglyceride and total chole-sterol concentrations in plasma and liver, HDL-cholesterol concentration in plasma, fecal total lipid triglyceride and total cholesterol excretions, thiobarbituric acid reactive substances (TBARS) level in plasma LDL + VLDL (low density lipoprotein + very low density lipoprotein) fractions, blood-clotting time and eicosanoids levels in plasma were measured. The results are as follows: Plasma total lipid, triglyceride and total cholesterol concentrations, TBARS level in plasma LDL + VLDL fractions were increased and blood-clotting time tended to be shortened during 3 months of experimental period. Low (5 ppm) iron diet improved lipid metabolism via increasing HDL-cholesterol and fecal choles-terol excretion. High (350 ppm) iron diet decreased plasma total lipid, triglyceride and total cholesterol concentrations as compared to medium (35 ppm) iron diet and lowered body weight and epididymal fat pad weight. On the other hand, TBARS level in plasma LDL + VLDL fractions and blood-clotting time were increased with high iron diet. It is plausible that low iron diet improves lipid metabolism, antioxidative and antithrombogenic capacities in 16-month-old rats.

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References

  1. 2001 Yearbook of Death Cause Statistic Statistical Office
  2. Aging Kim,S.H.;Kim,H.Y.
  3. Iron and human disease Lauffer,R.B.
  4. Wadsworth Gropper. Advanced Nutrition and Human Metabolism third edition Groff,J.L.;Sareen,S.
  5. J Clin Invest v.107 Iron overload diminishes atherosclerosis in apoE-deficient mice Kirk,E.A.;Heinecke,J.W.;LeBoeuf,R.C. https://doi.org/10.1172/JCI7664
  6. J Nutu Biochem v.14 Non-anemic iron deficiency, oral iron supplementation, and oxidative damage on college-aged females Sareen S. Gropper;Sata Kerr;Jerrey M. Barksdale https://doi.org/10.1016/S0955-2863(03)00072-X
  7. J Kor Nutr v.6 no.2 Effect of dietary iron and selenium levels on lipid levels of rats fed cholesterol diet Jung,Y.S.;Sung,C.J.
  8. Nutr Rev v.53 Iron overload-prevalence and impact on heat Lynch,S.R. https://doi.org/10.1111/j.1753-4887.1995.tb05482.x
  9. Circulation v.86 High stored iron levels are associated with excess risk of myocardial infarction in eastem Finnish men Salonen,J.T.;Nyyssonen,K;Korpela,H.;Tuomilehto,J.;Seppanen,R.;Salonen,R. https://doi.org/10.1161/01.CIR.86.3.803
  10. Am J Clin Nutr v.76 Iron stores and cadiovascular disease risk factors in women of reproductive age in United States Ramakishnan,U.;Kuklina,E.;Stein,A.D.
  11. Am J Clin Nutr v.76 Iron indexes and total antioxidant status in response to soy protein intake in perimenpopausal women Swain,J.H.;Aleker,D.L.;Dent,S.B.;Peterson,C.T.;Reddy,M.B.
  12. Nutrition across the life span KIm,U.K.;Lham,H.W.;Park,Y.S.;Myoung,S.O.;Lee,K.W.
  13. J Nutr v.123 AIN-93 Purified diets for laboratory rodents: final report of the American Institute Nutrition Ad Hoc Wrighting Committee on the reformulation of AIN-76A rodent diet Reeves,P.G.;Neilsen,F.H.;Fahey,G.C.
  14. Am J Clin Nutr v.53 A colorimetric method for determination of total serum liqid based on the sulfuric-phospho-vanillin reaction Frings,C.S.;Dunm,R.T.
  15. Can J Biochem Physiol v.37 A rapid method for total lipid extraction and purification Bigh,E.G.;Dyer,W.J. https://doi.org/10.1139/o59-099
  16. Lipids v.24 Determination of HDL₂cholesterol by precipitation with dextran sulfate and magnesium chloride establishing optimal conditions for rat plasma Sjoblom,L.;Eklund,A. https://doi.org/10.1007/BF02535134
  17. J Biol Chem v.193 Protein measurement with folin phenol regent Lowry,O.H.;Rosebrough,N.J.;Farr,A.L.;Randle,R.J.
  18. Med Sci Monit v.8 no.10 Very high aplha-tocopherol diet diminishes oxidative stress and hypercoagulation in hypertensive rats but not in normotensive rat Ferenoux,J.M.;Noirot,B.;Prost,E.D.;Modani,S.;Blond,J.P.;Prost,J.L.
  19. Fd Hyg Safety v.11 no.2 The antithrombotic effects of green tea, Catechin Yun,E.P.;Kang,O.S.;Lee,M.A.
  20. J Nutr v.130 Both iron deficiency and daily iron supplements increase lipid peroxidation in rat Michell,D.;Knutson,P.B.;Walter,B.
  21. Kor J Nutr v.35 no.7 Effect of grape intakes on lipid metabolism of rats during aging Um,M.Y.;Kim,M.K.
  22. Toxical Patho v.125 no.3 Spontaneous iron overload in Sprague Daweley rats Masson,R.;Roome,N.O
  23. J Nutr v.133 Iron overload in hyper cholesrelemic rats affects iom homeostasis and serum lipids but not blood pressure Turbina-Riberio SML;Silva,M.E.;Chianca,D.A.;De Paula H Jr;Cardos,L.M.;Colombari,E.;Pedosa,M.L.
  24. Nutr v.14 no.4 Effect of Hepatic Iron on Hypercholesterolemia and Hypeririacylglycerolemia in Copper-Deficient Fructose-Fed Rats Bureau,I.;Lewis,C.G.;Fields,M. https://doi.org/10.1016/S0899-9007(97)00490-5
  25. Molecular & cellular biology v.20 no.16 Coordinate transcriptional and translational regulation of ferritin response to oxidative stree Tsuji,Y.;Ayaki,H.;Whitman,S.P.;Morrow,C.S.;Torti,S.V.;Torti,F.M. https://doi.org/10.1128/MCB.20.16.5818-5827.2000
  26. J Clin Invest v.71 Hepatic lipid peroxidation in vivo in rats with chronic iron overload Bacon,B.R.;Tavill,A.S.;Brittenhan,G.M.;Park,C.H.;Recknagel,R.O. https://doi.org/10.1172/JCI110787
  27. Arch Pathol Lab Med v.103 Iron and the liver subcellular distribution of iron and decreased microsomal cytochrome P490 in livers of iron-loaded rats Bonkowsky,F.L.;Carpenter,S.J.;Healey,J.F.
  28. J Nutr v.132 High dietary iron concentrations enhance the formation of cholesterol oxidation products in the liver of adult rats fed salmon oil with minimal dffects on antioxidant status Brandsh,C.;Ringeseis,R.;Eeder,K.
  29. Am J Clin Nurt v.74 Flavonoids: a review of probable mechanisms of action and potential applications Njiveldt,R.J.;Nood,E.;Hoom,D.E.C.;Boelens,P.G.;Norren,K.;Leeuwen,P.A.M.
  30. Thrombosis Res v.78 no.2 Inhibition on platelet aggregation and eicosanoid production by phenolic components of olive oil Petroni,A.;Blasevich,M.;Salami,M.;Papini,N.;Montedoro,G.F.;Galli,C. https://doi.org/10.1016/0049-3848(95)00043-7
  31. Circulation v.170 Chronic iron administration increases vascular oxidative stress and accelerates arterial thrombosis Day,S.M.;Duquaine,D.;Lakshmi,V.;Menon,R.G.;Khan,B.V.;Rajagopalan,S.