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In Vivo Screening for Biocontrol Agents (BCAs) against Streptomyces scabiei Causing Potato Common Scab

  • Lee, Hyang-Burm (School of Biological Sciences, Seoul National University) ;
  • Cho, Jong-Wun (Korea Research Institute of Bioscience & Biotechnolog) ;
  • Park, Dong-Jin (Korea Research Institute of Bioscience & Biotechnolog) ;
  • Li, Chang-Tian (Korea Research Institute of Bioscience & Biotechnolog) ;
  • Ko, Young-Hwan (Department of Food Science and Engineering, Cheju National University) ;
  • Song, Jeong-Heub (Jeju-do Agricultural Research and Extension Service) ;
  • Koh, Jeong-Sam (College of Agricultural and Life Science, Cheju National University) ;
  • Kim, Bum-Joon (College of Medicine, Seoul National University) ;
  • Kim, Chang-Jin (Korea Research Institute of Bioscience & Biotechnology)
  • Published : 2004.06.01

Abstract

Through in vitro screening for biocontrol agents (BCAs) against Streptomyces scabiei causing potato (Solanum tuberosum) common scab, 19 streptomycete and 17 fungal isolates with antagonistic activity were selected as BCA candidates. For the selection of BCA candidates which are highly resistant to 10 kinds of antibiotics or pesticides, chemical susceptibility testing was initially performed in vitro. A remarkable degree of variation in susceptibility to antibiotics or pesticides was observed among the isolates tested. Streptomycete A020645 isolate was highly resistant to all the tested chemicals except neomycin up to 5,000 ppm. On the other hand, out of 36 antagonistic microbes subjected to in vivo pot tests using cultivar Daejima, four streptomycete isolates namely, A020645, A010321, A010564, and A020973, showed high antagonistic activity with >60% and 55% control value, respectively, and high chemical resistance to 10 kinds of chemicals. Therefore, these isolates were selected as potential BCAs for the control of potato common scab.

Keywords

References

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