Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
권호 표지

Ulva lactuca : A Potential Seaweed for Tumor Treatment and Immune Stimulation

  • Lee, Dong-Geun (Department of Bioscience and Biotechnology, Silla University) ;
  • Hyun, Jin-Won (Department of Biochemistry, College of Medicine, Cheju National University) ;
  • Kang, Kyong-Ah (Department of Biochemistry, College of Medicine, Cheju National University) ;
  • Lee, Jin-Ok (Department of Bioscience and Biotechnology, Silla University) ;
  • Lee, Sang-Hyun (Department of Bioscience and Biotechnology, Silla University) ;
  • Ha, Bae-Jin (Department of Bioscience and Biotechnology, Silla University) ;
  • Ha, Jong-Myung (Department of Bioscience and Biotechnology, Silla University) ;
  • Lee, Eun-Yeol (Department of Food Science and Technology, Kyungsung University) ;
  • Lee, Jae-Hwa (Department of Bioscience and Biotechnology, Silla University)
  • Published : 2004.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

This is the first report on the antitumor and immunostimulating activities of Ulva lactuca. Using the WSM (water-soluble fraction of a methanol extract from Ulva lactuca), a concentration of 140 g/mL was found to inhibit 50% of the human leukemia cell line U937 in growth, while splenocyte growth was stimulated up to a concentration of 100$\mu\textrm{g}$/mL. In addition, NO production by a macrophage cell line (RAW 264.7) and alkaline phosphatase activity in mouse splenocytes were both stimulated with 10$\mu\textrm{g}$/mL of WSM. Dose-dependent patterns were observed on all three cell-lines. Accordingly, the current results indicate that VUlva lactuca may be useful as a natural antitumor and immunostimulating agent.

Keywords

References

  1. J. Natl. Cancer Inst. v.77 Enhancement of pulmonary metastases induced by decreased lung natural killer cell activity Nolibe,D.;M.F.Poupon
  2. Blood v.76 Biology and clinical relevance of human natural killer cells Robertson,M.J.;J.Ritz
  3. Pharmacol. Rev. v.43 Nitric oxide: physiology, pathophysiology, and pharmacology Moncada,S.;R.M.Palmer;E.A.Higgs
  4. Annu. Rev. Biochem. v.63 Nitric oxide: a physiologic messenger molecule Bredt,D.S.;S.H.Synder https://doi.org/10.1146/annurev.bi.63.070194.001135
  5. Planta Med. v.55 Traditional herbal medicines-the role of polyphenols Haslam,E.;T.H.Lilley;Y.Cai;R.Martin;D.Magnolato https://doi.org/10.1055/s-2006-961764
  6. J. Nat. Prod. v.59 Natural polyphenols 9vegetable tannins) as drugs: Possible modes of action Haslam,E. https://doi.org/10.1021/np960040+
  7. Biosci. Biotech. Biochem. v.59 Macrophage stimulation activity of the polysaccharide fraction from a marine alga(Porphyra yezoensis): Structure-function relationships and improved solubility Yoshizawa,Y.;A.Ametani;J.Tsunchiro;K.Nomura;M.Ito;F.Fukui;S.Kaminogawa https://doi.org/10.1271/bbb.59.1933
  8. Caner. Lett. v.140 Potent suppressive effect of a Japanese edible seaweed, Enteromorpha prolifera (Sujiao-nori) on initiatio and promotion phases of chemically induced mouse skin tumorigenesis Hiqashi-Okaj,K.;S.Otani;Y.Okai https://doi.org/10.1016/S0304-3835(99)00047-6
  9. Int. J. Immunopharm v.19 B cell stimulating activity of seaweed extracts Liu,J.N.;Y.Yoshida;M.Q.Wang;Y.Okai;U.Yamashita https://doi.org/10.1016/S0192-0561(97)00016-7
  10. Free Radi. Biol. Med. v.32 Radiation sensitivity depends on OGG1 activity status in human leukemia cell lines Hyun,J.W.;G.J.Cheon;H.S.Kim;Y.S.Lee;E.Y.Choi;B.H.Yoon;J.S.Kim;N.H.Chung https://doi.org/10.1016/S0891-5849(01)00793-6
  11. Cancer Res. v.47 Evaluation of a tetrazolium-based semiautomated colorimetric assay: Assessment of chemosensitivity testing Carmichael,J.;S.G.DeGraft;A.F.Gazdar;J.D.Minna;J.B.Mitchel
  12. Biol. Pharm. Bull. v.25 In vitro and in vivo effects of macrophage-stimulatory polysaccharide from leaves of Perilla frutescens var. crispa Kwon,K.H.;K.I.Kim;W.J.Jun;D.H.Shin;H.Y.Cho;B.S.Hong https://doi.org/10.1248/bpb.25.367
  13. Biol. Pharm. Bull. v.24 Shark artilage as source of antiangiogenic compounds: From basic to clinical research Gonzalez,R.P.;A.Leyva;M.O.Moraes https://doi.org/10.1248/bpb.24.1097
  14. Biochem. Pharmacol. v.65 Oversulfation of fucoidan enhances its anti-angiogeni and antitumor activities Koyanagi,S.;N.Tanigawa;H.Nakagawa;S.Soeda;H.Shimento https://doi.org/10.1016/S0006-2952(02)01478-8
  15. Biol. Pharm. Bull. v.26 Apoptosis-inducing effect of a new bisphosphonate, YM529, on various hematopoietic tumor cell lines Nishida,S.;S.Kikuichi;H.Haga;S.Yoshioka;M.Tsubak;K.Fuji;K.Irimajiri https://doi.org/10.1248/bpb.26.96
  16. Biol. Pharm. Bull. v.25 Induction of nitric oxide synthase by Oldenlandia diffusa in mouse peritioneal macrophages Chung,H.S.;H.J.Jeong;S.H.Hong;M.S.Kim;S.J.Kim;B.H.Song;I.S.Jeong;E.J.Lee;J.W.Ahn;S.H.Baek;H.M.Kim https://doi.org/10.1248/bpb.25.1142