Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Cellulose Production from Gluconobacter oxydans TQ-B2

  • Shiru Jia (Department of Biochemical Engineering, Tianjin University of Science and Technology) ;
  • Hongyu Ou (Department of Biochemical Engineering, Tianjin University of Science and Technology) ;
  • Guibing Chen (Department of Biochemical Engineering, Tianjin University of Science and Technology) ;
  • Park, Du-Bok (Department of Environmental Engineering, Cho-dang University) ;
  • Cho, Ki-An (Department of Environmental Engineering, Cho-dang University) ;
  • Mitsuyasu Okabe (Biochemical Engineering Lab, Applied Biological Chemistry, Shizuoka University) ;
  • Cha, Wol-Suk (Department of Chemical Engineering, Chosun University)
  • Published : 2004.06.01
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Abstract

Gluconobacter oxydans that produces the cellulose was isolated. In order to confirm the chemical features of cellulose, various spectrophtometeric analysis were carried out using electron microscopy, X-ray diffractogram, and CP/MAS $\^$13/C NMR. The purified cellulose was found to be identical to that of Acetobacter xylinum. For effective production of cellulose, the various carbon and nitrogen sources, mixture of calcium and magnesium ions, and biotin concentration were investigated in flask cultures. Among the various carbon sources, glucose and sucrose were found to be best for the production of cellulose, with maximum concentration of 2.41 g/L obtained when a mixture of 10 g/L of each glucose and sucrose were used. With regard to the nitrogen sources, when 20 g/L of yeast extract was used, the maximum concentration of bacterial cellulose was reached. The concentration of cellulose was increased with mixture of 2 mM of each Ca$\^$2+/ and Mg$\^$2+/. The optimum biotin concentration for the production of cellulose was in the range of 15 to 20mg/L. At higher biotin concentration (25-35mg/L). the bacterial cellulose production was lower.

Keywords

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