Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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On-line Monitoring and Control of Substrate Concentrations in Biological Processes by Flow Injection Analysis Systems

  • Rhee, Jong-Il (BioProcess Technology lab., Faculty of Applied Chemical Engineering, Chonnam National University) ;
  • Adnan Ritzka (Institute of Technical Chemistry, University of Hannover) ;
  • Thomas Scheper (Institute of Technical Chemistry, University of Hannover)
  • Published : 2004.06.01
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Abstract

Concentrations of substrates, glucose, and ammionia in biological processes have been on-line monitored by using glucose-flow injection (FIA) and ammonia-FIA systems. Based on the on-line monitored data the concentrations of substrates have been controlled by an on-off controller, a PID controller, and a neural network (NN) based controller. A simulation program has been developed to test the control quality of each controller and to estimate the control parameters. The on-off controller often produced high oscillations at the set point due to its low robustness. The control quality of a PID controller could have been improved by a high analysis frequency and by a short residence time of sample in a FIA system. A NN-based controller with 3 layers has been developed, and a 3(input)-2(hidden)-1(output) network structure has been found to be optimal for the NN-based controller. The performance of the three controllers has been tested in a simulated process as well as in a cultivation process of Saccharomyces cerevisiae, and the performance has also been compared to simulation results. The NN-based controller with the 3-2-1 network structure was robust and stable against some disturbances, such as a sudden injection of distilled water into a biological process.

Keywords

References

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