The Growth Inhibition against Gastric Cancer Cell in Germanium or Soybean Sprouts Cultured with Germanium

게르마늄 및 게르마늄 분말 용해수로 재배한 콩나물의 위암세포 성장억제 작용

  • 김은정 (부산대학교 식품영양학과 및 김치연구소) ;
  • 이경임 (양산대학 호텔조리과) ;
  • 박건영 (부산대학교 식품영양학과 및 김치연구소)
  • Published : 2004.06.01

Abstract

The growth inhibitory effect of germanium, or soybean sprouts cultured with germanium, on cancer cells was determined in the cultured gastric cancer cell line, AGS. The growth of AGS was significantly inhibited by the addition of 0.01-1% organic germanium (Ge-132) and germanium stone powder in MTT cytotoxicity assays. The juice from germanium treated soybean sprouts (GTS) inhibited the growth of AGS gastric cancer cells by 78-88% at concentrations of 2.5 or 5${\mu}\ell$. The juice from Seomoktae GTS revealed an especially higher growth inhibitory effect than that from the control soybean sprouts (germanium non-treated soybean sprouts, GNTS) in AGS. The results suggest that soybean sprouts cultured with germanium may exert an anticancer effect against gastric cancer cells.

Keywords

References

  1. 윤택구 : 암의 원인과 환경. 한국 환경성 돌연변이 발암원 학회지, 1:39, 1981
  2. Doll, R and Pert, R : The cause of cancer. J. Natl.Cancer Inst., 66:1191, 1981
  3. Rhew, TH : Food, nutrition and cancer. J. Korean Soc.Food Nutr., 14:305, 1985
  4. 전세열 : 식품과 발암물질. 인간과학, 3(31):46, 1979
  5. Park, WK : Encyclopedia of foods and food science. p.403, Shin-kwang Publishing Corporation, Seoul, 1991
  6. Lee, YS, Park, RD and Rhee, CO : Effect of chitosantreatment on growing characteristics of soybean sprouts Korean J. Food Sci. Technol., 31:153, 1999
  7. Kim, YG, Im, TG, Park, SS, Heo, NC and Hong, SS :Effect of the DSSE(defatted sesame seed extracts) onquality characteristic of soybean sprouts. Korean J. FoodSci. Technol., 32:742, 2000
  8. Han, SS, Rim, YS and Jeong, JH : Growth characteristics and germanium absorption of soybeansprout cultured with the aqueous solution oforganogermanium. Agri. Chem. Biotechnol., 39:39, 1996
  9. Kim, EJ, Lee, KI and Park, KY : Effects of germaniumtreatment during cultivation of soybean sprouts. J. KoreanSoc. Food Sci. Nutr., 31:615, 2002 https://doi.org/10.3746/jkfn.2002.31.4.615
  10. Kim, EJ, Lee, KI and Park, KY : Quantity analysis ofnutrients in soybean sprouts cultures with germanium. J.Korean Soc. Food Sci. Nutr. 31:1150, 2002 https://doi.org/10.3746/jkfn.2002.31.6.1150
  11. Kim, EJ : Characteristics and cancer preventive effects ofgermanium soybean sprouts. Master thesis, Pusan NationalUniversity, 2000
  12. Goldin, A, Schepartz, SA, Venditti, JM and DeVita, VT: Historical development and current strategy of theNational Cancer Institute drug development program. InMethods in Cancer Research, Vol. 16. Cancer DrugDevelopment, Part A, DeVita VT and Busch H (eds.),Academic Press, New York, P.165, 1979
  13. Park, JG, Frucht, H, LaRocca, RV, Bliss, DP, Kurita, Y, Chen, TR, Henslee, JG, Trepel, JB, Jensen, RT,Johnson, BE, Bang, YJ, Kim, JP and Gazdar, AF :Characterization of cell lines established from humangastric carcinoma. Cancer Res., 50:2773, 1990
  14. Hwang, WI, Cha, S and Lee, SY : A study on theextraction of anticancer components from koreanmedicinal plants and the determination of their cytotoxicactivities on the cancer cells. Korean Biochem. J., 13:25,1980
  15. Franceschi, RT, James, WM and Zerlauth, G : la,25-dihydroxyvitamin $D_3$ specific regulation of growth,morphology and fibronectin in a human osteosarcoma cellline. J. Cellular Physiol. 123:401, 1985 https://doi.org/10.1002/jcp.1041230316
  16. Park, JG, Kramer, BS, Steinber, SM, Carnichael, J,Collins, JM, Minna, JD and Gazdar, AF :Chemosensitivity testing of human colorectal carcinomacell lines using a tetrazolium-based colorimetric assay.Cancer Res., 47:5875, 1987
  17. Kageyama, K, Onoyama, Y, Nakanishi, M, Matsui-Yuasa,I, Otani, S and Morisawa, S : Synergistic inhibition ofDNA synthesis in Enrlich ascites tumor cells by acombination of unsaturated fatty acids and hyperthermia.J. Applied Toxico. 9:1, 1989 https://doi.org/10.1002/jat.2550090102
  18. Steel, RGD and Torrie, JH : Principles and procedureof statistics. p.96, McGraw-Hill kogakusha, Ltd., Tokyo,1980
  19. Lee, HM, Chung, Y, Jung, KW, Kim, JW and Kwon,SK : Antimutagenic effect of organic germanium(Ge-132)on the mutagenicity of benzo(a)Pyrene. Yakhak Hoeji37:18, 1993