Effects of Conjugated Linoleic Acid Accumulated Pork on Fatty Acid Composition and Quality Characteristics of Press Ham

CLA가 축적된 돈육으로 제조된 Press Ham의 지방산 조성 및 품질 특성에 미치는 영향

  • Published : 2004.06.01

Abstract

The CLA used to add in diet was chemically synthesized by alkaline isomerization method with corn oil. Pigs were divided into 5 treatment groups (4 pigs/group) and subjected to one of five treatment diets (0, 1.25% CLA for 2weeks, 2.5% CLA for 2weeks, 1.25% CLA for 4weeks and 2.5% CLA for 4weeks, CLA diets; total fed diets) before slaughter. Pork loin were collected from the animals (110kg body weight) slaughtering at the commercial slaughter house. Manufacture press ham using CLA accumulated pork loin were vacuum packaged and then stored during 1, 7, 14, 21 and 28 days at 4$^{\circ}C$. Samples were analyzed for texture characteristics, sensory evaluation, fatty acid composition and CLA content. There was no significant difference in texture among control and CLA treatment groups. It was not clearly changed by storage time. No remarkable differences were found in sensory properties (color, flavor, texture and acceptability) between control and CLA treatment groups. It was not clearly changed by the passage of storage time. In the change of fatty acid composition, the contents of oleic, linoleic and arachidonic were decreased by dietary CLA-supplementation, whereas the increase level of CLA-supplementation resulted in the higher palmitic and stearic acid than control. As dietary CLA was increased in feed, the content of CLA in pork was increased.

CLA는 alkaline isomerization 방법으로 식용유를 이용하여 전체 CLA 이성체가 56%인 CLA를 화학적으로 합성하였다. 대조구는 80-110kg까지는 일반 사료회사에서 생산하는 사료를 급여하였으며, 처리구 1은 출하(출하체중 : 110kg) 2주전부터 일반사료회사에서 생산하는 사료에 CLA를 1.25% 첨가하여 급여하였으며, 처리구 2는 출하 2주전부터 사료함량에 CLA를 2.5%첨가하여 급여하였다. 처리구 3은 사료함량에 1.25% CLA를 첨가하여 4주간 급여하였으며, 처리구 4는 2.5% CLA를 4주간 급여하였다. 급여기간이 끝난 후 일괄적으로 함양도축장에서 도축하여 좌 우도체 의심부위를 발골ㆍ정형하여 제조 원료로 사용하여 프레스햄을 제조한 후 진공 포장하여 냉장온도(4$^{\circ}C$)에서 1, 7, 14, 21 및 28일간 저장하면서 조직적 특성분석, 관능적 특성분석, 지방산 조성 및 CLA 함량 등을 조사하여 CLA가 축적된 프레스햄의 저장기간에 따른 품질 특성을 규명하고자 실시하였다. 조직감의 변화에서 저장기간별 처리구간의 비교에서 유의적인 차이는 인정되었지만 뚜렷한 경향이 없었으며, 저 장기간의 경과에 따른 변화에서도 뚜렷한 변화가 없었다. 관능검사 중 육색의 변화는 전 저장기간 동안 대조구와 비교하여 CLA급여 처리구가 다소 높은 육색을 보였다. 향기는 전 저장기간 동안 처리구간에 유의적인 차이가 없었으며, 조직감은 처리구간에 유의적인 차이가 인정되었는데, 저장 1일과 7일에는 대조구가 CLA급여 처리구인 T3, T4에 비하여 유의적으로 높은 조직감을 보였다.(p<0.05). 전체적인 기호성은 저장 1일에 대조구에 비하여 CLA 급여 처리구가 유의적으로 높은 기호성을 보였다(p<0.05). 저장 1일 이후에는 CLA 급여 처리구가 대조구에 비하여 높은 평가를 받았지만 유의적인 차이는 없었다. 저장기간에 따른 제품의 육색, 향기, 조직감, 전체적인 기호성 변화는 모든 처리구가 저장기간이 경과하여도 유의적인 차이가 없었다. 대조구에 비하여 CLA 급여 처리구는 myristic, palmitic 및 stearic acid함량은 유의적으로 증가하며(p<0.05), 반면에 oleic, linoleic 및 arachidonic acid 함량은 유의적으로 감소하였다(p<0.05). CLA 함량을 보면 CLA를 급여하지 않은 대조구는 CLA가 검출되지 않았지만 CLA 급여 처리구에서는 CLA 급여수준과 급여기간이 증가할수록 육내 축적율이 직선적으로 증가하였다. 포화지방산과 불포화지방산 함량의 비는 전 저장기간동안 대조구와 비교하여 CLA 급여 처리구가 낮은 불포화지방산 함량을 보였다. 이와 같은 결과는 CLA 급여로 인하여 oleic acid 함량이 감소하였기 때문인 것으로 사료된다. 이상의 결과 프레스햄 제조시 CLA가 축적된 돈육의 이용은 조직감 및 관능적 평가에 영향을 미치지 않으며, CLA를 축적시키는 것이 가능하므로 고급 육제품 생산이 가능하다고 사료된다.

Keywords

References

  1. Belury, M. A. (1995) Conjugated dienoic linoleate: a polyunsaturated fatty acid with unique chemoprotective properties. Nutr. Rev. 53, 83-89 https://doi.org/10.1111/j.1753-4887.1995.tb01525.x
  2. Chin, S. F., Liu, W., Storkson, J. M., Ha, Y. L., and Pariza, M. W. (1992) Dietary sources of conjugated dienoic isomers of linoleic acid, a newly recogenized class of anticarcinogens. J. Food Composition and Analysis 5, 185-197 https://doi.org/10.1016/0889-1575(92)90037-K
  3. Cook, M. E., Miller, C. C., Park, Y., and Pariza, M. W. (1993) Immune modulation by altered nutrient metabo-lism: nutritional control of immune induced growth depression. Poult. Sci. 72, 1301-1305 https://doi.org/10.3382/ps.0721301
  4. Doyle, E. (1998) Scientific forum explores CLA know-ledge. INFORM 9, 69-73
  5. Du, M., Ahn, D. D., Nam, K. C., and Sell, J. L. (2000) Influence of dietary conjugated linoleic acid on volatile profiles, color and lipid oxidation of irradiated raw chicken meat. Meat Sci. 56, 387-395 https://doi.org/10.1016/S0309-1740(00)00067-X
  6. Dunshea, F. R, Ostrowska, E., Luxford, B., Smits, R. J., Campbell, R. G., D'Souza, D. N., and Mullan, B. P. (2002) Dietary conjugated linoleic acid can decrease backfat in pigs housed under commercial conditions. Asian-Aust. J. Anim. Sci. 15, 1011-1017
  7. Eggert, J. M., Belury, M. A., Kempa-Steczko, A., Mills, S. E., and Schinckel, A. (2001) Effects of conjugated linoleic acid on the belly firmness and fatty acid composition of genetically lean pigs. J Anim. Sci. 79, 2866-2872
  8. Eggert, J. M., Carroll, A. L., Richert, B. T., Gerrard, D. E., Forrest, J. C., Bowker, B. C., Wynveen, E. J., Hammelman, J. E., and Schinckel, A. P. (1999) Effects of conjugated linoleic acid(CLA) on the growth, carcass composition and pork quality of two genotypes of lean gilts. J Anim. Sci. 77, 178(abstr)
  9. Fitch, H. B. (1996) Conjugated linoleic acid offers research promise. INFORM. 7, 152-159
  10. Fogerty, A. C., Ford, G. L., and Svoronos, D. (1988) Octadeca-9,II-dienoic acid in food stuffs and in the lipids of human blood and breast milk. Nutrition Reports International 38, 937-944
  11. Folch, J., Lees, M., and Sloane-Stanley, G. H. (1957) A simple method for the isolation and purification of total lipid from animal tissues. J. BioI. Chem. 226, 497-509
  12. Fulghum, J. G., Sebranek, J. G., Jr. Parrish, F. C., and Wiegand, B. R. (1999) Effects of feeding conjugated linoleic acid to market pigs on bacon quality and composition. 1998. Swine Res. Rep., Iowa State Univ., Ames. pp. 231-232
  13. Ha, Y. L., Grimm, N. K., and Pariza, M. W. (1987) Anticarcinigens from fried ground beef: Heat-altered derivatives of linoleic acid. Carcinogenesis 8, 1881-1887 https://doi.org/10.1093/carcin/8.12.1881
  14. Ha, Y. L., Park, G. B., Kang, S. J., Shim, K. H., and Kim, J. O. (1998) Newly recongnized multifuctional fatty acids for the production of high quality meat, fish and agricultural products. Ministry of Agriculture and Fores-try(Report), pp. 1-314
  15. Houseknecht, K. L., Vanden Heuvel, J. P., Moya-Camarena, S. Y., Portocarrero, C. P., Peck, L. W., Nickel, K. P., and Belury, M. A. (1998) Dietary conjugated linoleic acid normalizes impaires glucose tolerance in the Zucker diabetic fatty fa/fa rat. Biochem. Biophys. Res. Comm. 244, 678-682 https://doi.org/10.1006/bbrc.1998.8303
  16. Hughes, P. E., Hunter, W. J., and Tove, S. B. (1982) Biohydrogenation of unsaturated fatty acids. Purification and properties of cis,9-transoctadeca-dienoate reductase. J. Biol. Chem. 257, 3643-3649
  17. Joo, S. T., Lee, J. I., Ha, Y. L., and Park, G. B. (2002) Effects of dietary conjugated linoleic acid on fatty acid composition, lipid oxidation, color and water-holding capacity of pork loin. J. Anim. Sci. 80, 108-112
  18. Karmer, J. K. G., Sehat, N., Dugan, M. E. R., Mossoba, M. M., Yurawecz, M. A., and Ku, Y. (1998) Distribu-tion of conjugated linoleic acid(CLA) isomers in tissue lipid classes of pigs fed a commercial CLA mixture determined by GC and silver ion-HPLC. Lipids 33, 549-558 https://doi.org/10.1007/s11745-998-0239-1
  19. Larsen, S. T., Wiegand, B. R., Jr. Parris, F. C., and Sparks, J. C. (1999) Effects of CLA supplementation on ham quality characteristics of crossbred growing-finishing barrows. J. Anim. Sci. 77, 47(abstract)
  20. Lee, J. I., Joo, S. T., Park, T. S., Shin, T. S., Ha, Y. L. and Park, G. B. (1999) Changes in physico-chemical properties of accumulated broiler by conjugated linoleic acid(CLA) during cold storage. Kor. J. Food Sci. Ani. Resour. 19, 88-99
  21. Lee, K. N., Kritchevsky, D., and Pariza, M. W. (1994) Conjugated linoleic acid and atherosclerosis in rabbits. Atherosclerosis. 108, 19-25 https://doi.org/10.1016/0021-9150(94)90034-5
  22. Lee, K. S. (1996) Conjugated linoleic acid and lipid meta-bolism. Ph.D. Thesis at the University of Wisconsin-Madison. pp. 77-120
  23. Lin, H., Boylston, T. D., Chang, M. J., Luedecke, L. O., Shultz, T. D., and Boylston, T. D. (1995) Survey of the conjugated linoleic acid contents of dairy products. J. Dairy Sci. 78, 11
  24. Miller, C. C., Park, Y., Pariza, M. W., and Cook, M. E. (1994) Feeding conjugated linoleic acid to animals partially overcomes catabolic response due to endotoxin injection. Biochem. Biophys. Res. Commun. 198, 1107-1112 https://doi.org/10.1006/bbrc.1994.1157
  25. Mossoba, M. M., McDonald, R. E., and Armstrong, D. J. (1991) Identification of minor c18 triene and conjugated diene isomers in hydrogenated soybean oil and margarine by GC-MI-FT-IR spectroscopy. J Chromatogr. Sci. 29, 324-330 https://doi.org/10.1093/chromsci/29.8.324
  26. Nicolosi, R. J., Rogers, E. J., Kritchevsky, D., Scimeca, J. A., and Huth, P. J. (1997) Dietary conjugated linoleic acid reduces plasma lipoproteins and early aortic athero-sclerosis in hypercholesterolemic hamsters. Artery. 22, 266-277
  27. Park, G. B., Lee, J. I., Ha, Y. L., Kang, S. J., Jin, S. K., and Joo, S. T. (1998) Effect of conjugated linoleic acid on fatty acid composition and lipid oxidation of egg yolk. Kor. J. Food Sci. Ani. Resour. 18, 339-347
  28. Pettigew, J. E. and Esnaola, M. A. (2001) Swine nutrition and pork quality: A review. J Anim. Sci. 79, E316-E342
  29. SAS. (1999) SAS/STAT Software for PC. Release 6.11, SAS Institute, Cary, Nc, U.S.A.
  30. Thiel, R. L., Sparks, J. C., Wiegand, B. R, Jr. Parrish, F. C., and Ewan, R. C. (1998) Conjugated linoleic acid improves performance and body composition in swine. J. Anim. Sci. 76, 57(abstract)
  31. Wiegand, B. R. Jr., Parrish, F. C., and Sparks, J. C. (1999) Effects of CLA supplementation on pork quality characteristics in crossbred growing-finishing barrows. J Anim. Sci. 77, 47(abstract)