Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Enzymatic Synthesis of Functional Oil from Rice Bran Oil and Dietary Effects on Hepatic ACAT Activities of High Cholesterol and High Fat Fed Mice

현미유를 이용한 기능성 유지의 효소적 합성 및 고 콜레스레롤, 고 지방 식이가 생쥐의 간 ACAT활성에 미치는 영향

  • Published : 2004.06.01
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Abstract

Monoacylglycerol (MG) and diacylglycerol (DG), as the components of enzymatically synthesized functional oil, were produced by glycerolysis of rice bran oil and glycerol using IM60 (immobilized lipase) in a stirredbatch reactor at 6$0^{\circ}C$ for 72 hours. After glycerolysis, the contents of triacylglycerol (TG), DG and MG in the produced functional oil were 41.71%,46.19%, and 11.15%, respectively. The functional oil also contained Phytosterols (2.04$\pm$0.17 mg/g), ${\gamma}$ -oryzanol (1.06$\pm$0.04 mg/g) and $\alpha$ -tocopherol (0.13$\pm$0.04 mg/g). In animal experiment the dietary effects of functional oil on hepatic acyl-CoA:cholesterol acyltransferase (ACAT) activities of the high cholesterol and high fat (HCHF)-fed mice were investigated. In functional oil-fed group, the liver ACAT activity was significantly lowered than in HCHF and corn oil-fed groups (p < 0.05). This results suggested that the synthesized functional oil may have an atheroproteetive effect by inhibiting ACAT activity.

본 실험에서는 DG, MG를 함유한 기능성 유지를 합성하기 위해 lipase(IM 60)를 이용하여 현미유와 글라이세롤을 회분식 교반기에서 glycerolysis화 반응을 하였다. 합성한 기능성 유지와 현미유의 이화학적, 영양학적 특성 을 비교하기 위해 지방산 조성, 기능성 물질 함량 및 동물 식이 실험을 실시하였다. 실험 결과 기능성 유지는 DG(46.19%), MG(11.15%)와 7G(41.71%) 그러고 미량의 자유 지방산으로 구성되어 있었으며, 지방산 조성은 현미유와 흡사하였다. 비검화 물질함량 또한 현미유와 유의적 차이를 보이지 않았으며, 주요물질로써 Phytosterol(2.04$\pm$0.17 mg/g), ${\gamma}$-oryzano1(1.06$\pm$0.04 mg/g), $\alpha$ -tocopherol(0.13$\pm$0.04 mg/g)이 검출되었다. 동물 식이실험 에서는 기능성 유지와 현미유 식이군이 ACAT activity를 저해하였으며, HCHF 식이군과 옥수수유 식이군 보다 유의적으로 낮게 나타낮다(p<0.05). 간과 지방 조직의 지방산 조성은 기능성 유지를 비롯한 모든 식이군에서 oleic acid의 함량이 가장 높았다.

Keywords

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