Korean journal of applied entomology (한국응용곤충학회지)

Korean Society of Applied Entomology (한국응용곤충학회)
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A Possible Mechanism Related with Non-spinning Syndrome of Bombyx mori that Intimidates the Sericultural Industry in Northern Kyungbuk

경북 북부지역의 양잠산업에 피해를 주고 있는 누에(Bombyx mori) 미화용 기작에 관한 연구

  • Kim, Yong-Kyun (Department of Agricultural Biology, Andong National University) ;
  • Bae, Sang-Ki (Department of Agricultural Biology, Andong National University) ;
  • Lee, Sun-Young (Department of Agricultural Biology, Andong National University) ;
  • Ji, Dong-Jin (Department of Agricultural Biology, Andong National University) ;
  • Kim, Jin (Central Research Institute, Kyung-Nong Corporation) ;
  • Hong, Yong-Pyo (Department of Applied Chemistry, Andong National University) ;
  • Kim, Gil-Ho (Department of Sericulture & Entomology, Sangju National University)
  • 김용균 (안동대학교 자연과학대학 농생물학과) ;
  • 배상기 (안동대학교 자연과학대학 농생물학과) ;
  • 이선영 (안동대학교 자연과학대학 농생물학과) ;
  • 지동진 (안동대학교 자연과학대학 농생물학과) ;
  • 김진 ((주)경농 중앙연구소) ;
  • 홍용표 (안동대학교 자연과학대학 응용화학과) ;
  • 김길호 (상주대학교 농학부 잠사곤충자원학과)
  • Published : 2004.06.01
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Abstract

Non-spinning syndrome of Bombyx mori has been serious issue in sericulture industry near Kyungbuk area. This study was focused on the analysis of the mechanism and on screening candidate chemicals inducing the anti-metamorphosis of the silkworms. Rearing temperatures or initial body weight of the final instar larvae did not affect a normal larval to pupal metamorphosis of B. mori. However, pyriproxyfen (a juvenile hormone (JH) agonist) induced follicle patency significantly even at its 10$\^$-8/ M concentration and inhibited metamorphosis of B. mori in both developmental time and dose dependent manners. Pyriproxyfen induced JH esterase (JHE) activity and downregulated expression of JH binding protein of 5. mori. These results suggests that pyriproxyfen induced JHE activity as a JH agonist and that the elevated JHE activity degraded endogenous JH and resulted in JHBP gene expression. Based on the fact that the JH agonist induced follicle patency and inhibited metamorphosis of B. mori, follicle patency bioassay suggested that three commercial pesticides including simazine, molinate or alachlor were proved to give potent JH agonistic effect on B. mori. Further direct exposure experiments to these candidates are required to determine the chemicals responsible for the non-spinning syndrome of 8. mori.

경북 북부지역의 양잠농가에 피해를 주고 있는 누에(Bombyxmnri)의 미화용 유발 문제에 대한 원인과 기작을 밝혀내는 데, 이 연구의 궁극적 목적을 두었다. 일반 사육환경에서 나타날 수 있는 발육온도(20, 25, 3$0^{\circ}C$)와 최종령 초기 유충 체중 변이(412.6-1,616.0 mg)는 누에의 용화 결정에 영향을 주지 많았다 한편 유약호르몬(juvenile hormone：計I)동력제인 피리프록시팬(pyriproxyfen)은 10-s M의 낮은 농도에서도 난포세포 개방화를 유도하여, 이 약제가 누에에서도 동력제라는 것을 확인하였다. 피리프록시펜은 약제 농도 및 처리 시기별로 특이하게 누에 미화용 유발 효과를 보였다. 피리프록시펜은 JH 에스테라제(JH esterase： JHE)를 직접적으로 억제하지 못하고, 오히려 활성을 증가시키는 것으로 나타났으며, JH 결합단백질(JH binding protein：JHBP)의 유전자 발현을 억제하는 것으로 나타났다. 즉, 피리프록시펜은 체내에 JH 동력제로 작용하면서 JHE 유전자 활성을 제고시켰지만, JHE활성 증가에 따른 체내 JH함량이 줄어듬에 따라 JHBP의 유전자 활성을 억제하는 것으로 해석된다. 양잠농가 주변으로 살포 가능성이 있는 농약을 대상으로 난포세포 개방화 분석을 실시 한 결과, 제초제 3종(simazine, molinate, alachlor)이 JH 동력제 효과를 가지고 있는 것으로 나타났다. 누에 미화용 원인체 규명을 위해서 이들에 대한 직접 노출 시험이 요구된다.

Keywords

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