RAPD를 이용한 한국산 줄장지뱀(Reptilia: Squamata)의 종내 다양성에 관한 연구

Intraspecific Diversity of Korean Takydromus wolteri(Reptilia: Squamata) Based on Randomly Amplified Polymorphic DNA (RAPD) Analysis

  • 발행 : 2004.06.01

초록

한반도 전지역에 줄장지뱀이 서식하고 있지만, 이 종에 대한 연구는 거의 전무한 상태이다. 한반도의 5지역(경기도, 충청북도, 제주도, 전라남도, 경상남도)의 한국산 줄장지뱀에 대해 RAPD method를 통한 종내 유전적 차이를 비교하였다. 총 28개의 primer를 사용하였고, 그 중 유의성이 있는 17개의 primer에 대한 결과를 Nei's(1972) genetic distance를 통해 유전적 거리와 UPGMA 방법을 통한 phenogram을 구하였다. 그 결과 총 68개의 밴드를 확인했고,이 중 87%인 59개의 polymorphism이 확인되었다. 이를 통한 phenogram에서는 GG1, GG2, CB1, CB2, JN이 Group 1을 이루었으며,JJ1, JJ2, GN이 Croup 2를 이루었다. 줄장지뱀의 경우 지리적 격리를 통한 집단간의 종내 변이가 발생한 것으로 추정된다. 특히, 경상남도 지방의 유전적 독립성이 두드러지는데, 이는 다른 분류군인 어류나 양서류에서도 나타나고 있다. 따라서 이들 집단에 대한 형태적, 생태적 그리고 다른 유전적 분석 등을 통한 추가적인 연구가 필요할 것이라고 판단된다.

The lacertid lizard Takydromus wolteri widely distributed in South Korea, but intraspecific diversity of this species was not almost studied. T. wolteri represented by 8 specimens collected from 5 localities were analyzed by the Randomly Amplified Polymorphic DNA (RAPD) method with 28 random decamer primers and the phenogram constructed by using the Unweighted Pair Group Method with Arithmetic Mean (UPGMA) method based on RAPD data. The populations of T. wolteri using in this paper were collected from Gyeonggi-do, Chungcheongbuk-do, Jeju-do, Jeollanam-do and Gyeongsangnam -do in South Korea. Among 68 different bands detected in RAPD analysis, 59 bands (87%) showed polymorphism. The phenogram showed that the populations of T. wolteri were grouped into two. These results suggest that T. wolteri were supported intraspecific diversity.

키워드

참고문헌

  1. 한국동식물도감제 17권 동물편(양서.파충류) 강영선;윤일병
  2. 뱀 백남극;심재한
  3. 꿈꾸는 푸른 생명 거북과 뱀 심재한
  4. 한국의 양서류 양서영;김종범;민미숙;서재화;강영진
  5. 고흥(8-18)의 자연환경 고흥(팔영산)일대의 포유류 및 양서, 파충류 한상훈;송재영
  6. Zool. J. Linn. Soc. v.119 Interrelationships and evolution of the east Asian grass lizards, Takydromus (Squamata: Lacerticdae) Arnold EN https://doi.org/10.1111/j.1096-3642.1997.tb00138.x
  7. Chosen Iho. Takydromus kwangakuensis sp. nov. from Korea Doi H.
  8. J. Chosen Nat. His. Soc. v.9 A new species of Tokydromus from Corea Doi H.
  9. PCR technology : current innovations Grifin HG;AG Griffin
  10. Natural history of the reptiles Hikida T.
  11. Quaternary Res. v.29 The history of straits around the Japanese islands in the Late-quaternary Kazuo O. https://doi.org/10.4116/jaqua.29.193
  12. Korea J. Zool. v.34 Geographic variation of lamina circularis in Cobitis longicorpus (Pisces: Cobitidae) Kim JB;JM Kim;SY Yang
  13. Zool. Sci. v.20 A new species of lentic breeding Korea salamander of the genus Hynobius(Amphibia, Urodela) Kim JB;MS Min;M Matsui https://doi.org/10.2108/zsj.20.1163
  14. Korea Syst. Zool. v.12 Systematic studies on the freshwater goby, Rhinogobius species (Perciformes, Gobiidae). II. Geographic distribution and taxonomic status of three color types in the Rhinogobius brunneus complex from South Korea Kim JM;SY Yang
  15. Mol. Phylogenet. Evol. v.22 Molecular phylogeny and biogeography of the grass lizards Genus Takydromus (Reptilia: Lacertidae) of east asia Lin SM;CA Chen;KY Lue https://doi.org/10.1006/mpev.2001.1059
  16. Natural history of the amphibian Matsui M
  17. Korea J. Zool. v.34 Systematic study on the genus Zacco (Pisces, Cyprinidae). I. Genetic variation Min MS;SY Yang
  18. Amer. Natur. v.106 Genetic distance between populations Nei M https://doi.org/10.1086/282771
  19. Biol. J. Linn. Soc. v.76 Phylogenetic relationships, taxonomy, character evolution and biogeography of the lacertid lizards of the genus Takydromus (Reptilia: Squamata): a molecular perspective Ota H. https://doi.org/10.1046/j.1095-8312.2002.00084.x
  20. Korean J. Zool. v.22 Biochemical variation and systematic status of the genus Agkistrodon (Crotalidae) in Korea Paik NK;YJ Kim;SY Yang
  21. Korean J. syst. Zool. v.2 Taxonomic study on two subspecies of European grass snake (Rhabdophis tigrina) in Korea Paik NK:SY Yang
  22. Korean J. syst. Zool. v.5 Geographic variation and interspecific hybridization between two species of the genus Agkistrodon (Crotainae) in Korea Paik NK:SY Yang
  23. Koean J. Genet. v.12 Heteroplasmy in mitochondrial DNA of Chinese viper, Agkistrodon blomhoffii brevicaudus Paik NK;HY Lee;EK Jung
  24. Korean J. Genet. v.14 Genetic differentiation of mitochondrial DNA in the genera, Enhydris and Elaphe Paik NK;HY Lee;EK Jung;IS Kim
  25. Geology of Korea Continental shelf sedmentation Park YA;Lee D.S.(ed.)
  26. Russian J. Genet. v.34 Polymorphism of RAPD Markers in lizard Populations of Family Lacertidae Ryabinina NL;VV Grechko;IS Darevskii
  27. Molecular cloning Sambrook J;DW Rusell
  28. Herpetologica v.12 The reptiles and amphibians of Korea Shannon FA
  29. Korean J. Nat. Con. v.I Herpetofauna biodiversity and ecological research at the Mt. Cheondeung, Chungjusi Shim JH;SC Lee
  30. U.S. Nat. Mus. v.58 Herpetology of Japan and adjacent territory Stejneger L.
  31. PAUP$^*$: Phylogenetic analysis using parsimony ($^*$and other methods). V.4.0b8 Swoffrod DL
  32. Copeia v.1958 The status of Takydromus kwangakuensis Walley HD
  33. Herpetologica v.14 A new lacertid lizard from Korea Walley HD
  34. Herpetologica v.18 The identity of Korea lizard, Takydromus auroralis Doi Walley HD
  35. Korean J. Zool. v.32 Genetic variation and systematics of the sticklebacks (Pisces, Gasterosteidae) in Korea Yang SY;MS Min