Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
권호 표지

Antibody Response to Crude Cell Lysate of Propionibacterium acnes and Induction of Pro-Inflammatory Cytokines in Patients with Acne and Normal Healthy Subjects

  • Basal, E. (Department of Microbiology, KGMU, Lucknow, INDIA) ;
  • Jain, A. (Department of Microbiology, KGMU, Lucknow, INDI) ;
  • Kaushal, G.P. (Department of Medicine, KGMU, Lucknow, INDIA)
  • Published : 2004.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

Propionibacterium acnes (P. acnes) plays an important role in the disease pathogenesis of acne vulgaris, a disorder of pilosebaceous follicles, seen primarily in the adolescent age group. In the present study, the presence of antibodies against P. acnes (MTCC1951) were detected in acne patient (n=50) and disease free controls (n=25) using dot-ELISA and Western blot assay. The ability of P. acnes to induce pro-inflammatory cytokines by human peripheral blood mononuclear cells (PBMCs), obtained from acne patients and healthy subjects, were also analysed. The patients (n=26) who were culture positive for skin swab culture, were found to have a more advanced disease and higher antibody titres (1:4000 to >1:16000) compared to the P. acnes negative patients (n=24) and normal controls (n=25). An analysis of patients' sera by western blot assay recognized a number of antigenic components of P. acnes, rang-ing from 29 to 205 kDa. The major reactive component was an approximately 96 kDa polypeptide, which was recognised in 92％ (24 of 26) of the patients sera. Further, the P. acnes culture supernatant, crude cell lysate and heat killed P. acnes whole cells, obtained from 72-h incubation culture, were observed to be able to induce significant amounts of IL-8 and tumor necrosis factor alpha (TNF-${\alpha}$) by the PBMCs in both the healthy subjects and patients, as analysed by cytokine-ELISA. The levels of cytokines were significantly higher in the patients than the healthy subjects. A major 96 kDa polypep-tide reactant was eluted from the gel and was found to cause dose dependent stimulation of the pro-ductions of IL-8 and TNF-${\alpha}$. Thus, the above results suggest that both humoral and pro-inflammatory responses play major roles in the pathogenesis of acne.

Keywords

References

  1. Methods Enzymol. v.91 High-sensitivity sequence analysis of protein recovered from sodium dodecyl sulphate gels Bhown, A.S.;J.C. Bennett https://doi.org/10.1016/S0076-6879(83)91042-X
  2. Cell Immunol. v.17 Mechanisms of macrophage activation by Corynebacterium parvumⅡ.in vivo experiments Bomford, R.;G.H. Cristie https://doi.org/10.1016/S0008-8749(75)80015-3
  3. Scand J. Clin. Lab. Invest. no.Suppl. 97 Isolation of mononuclear cells and granulocytes from human blood. Isolation of mononuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentatiom at l g Boyum, A.
  4. Anal. Biolchem. v.72 A rapid and sensitivity method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Bradford, M.M. https://doi.org/10.1016/0003-2697(76)90527-3
  5. J. Gen. Microbial. v.80 Corynebacterium parvum: a synonym of Propionibacterium acnes? Cummins, C.S.;J.L. Johnson https://doi.org/10.1099/00221287-80-2-433
  6. Acne: Natural History of Acne Cunliffe, W.J.
  7. Lancet v.1 On the etiology of acne vulgaris and its treatment by vaccines Fleming, A.
  8. J. Biol. Chem. v.270 Regulation of vascular endothelial growth factor expression in cultured keratinocytes. Implications for normal and impaired wound healing Frank, S.;G. Hubner;G. Breier;M.T. Longaker;D.G. Greenhalgh;S. Werner https://doi.org/10.1074/jbc.270.21.12607
  9. Arch. Dermatol. Res. v.275 Propionibacteria in patients with acne vulgaris and in healthy persons Gehse, M.;U. Hoffler;M. Gloor;G. Pulverer https://doi.org/10.1007/BF00412883
  10. J. Reticuloendothel Soc. v.27 The activation of macrophages by Corynebacterium parvum: effect of anti-complemetary agents cobra venom factor and sodium cyanate Ghaffar, A.
  11. Ann. Clin. Res. v.11 Evaluation of a culture procedure for identification of Neisseria gonorrhoeae Hellgren, L;G. Johannison;J. Vincent.
  12. Exp. Dermatol. v.2 Detection of Propionibacterium acnes polypeptides, which have stimulated an immune response in acne patients but not in normal individuals Holland, K.T.;D.B. Holland;W.J. Culiffe;A.G. Cunliffe https://doi.org/10.1111/j.1600-0625.1993.tb00193.x
  13. Br. J. Dermatol. v.116 Antibodies to P. acnes and p. acnes exocellular enzymes in the normal population at various ages and in patients with acne vulgaris Ingharm, E;G. Growland;R.M. Ward;K.T. Holland;W.J. Cunliffe https://doi.org/10.1111/j.1365-2133.1987.tb04899.x
  14. Arch. Dermatol. Res. v.277 Isolation of an acidic polysaccharide antigen from Propionibacterium acnes Iversen, O.J.;A.B. Dalen;H.B. Svindland https://doi.org/10.1007/BF00404321
  15. Dermatology v.196 Vascular endothelial growth factor expression induced by proinflammatory cytokines (interleukin 1 alpha, beta) in cells of the human pilosebaceous unit Kozlowska, U.;U. Blume-Peytavi;V. Kodelja;C. Sommer;S. Goerdt;S. Jablonska;G.E. Orfanos https://doi.org/10.1159/000017878
  16. Nature v.227 Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Laemmli, U.K. https://doi.org/10.1038/227680a0
  17. J. Med. Microbiol. v.20 The pathological and ecological significance of microorganisms colonizing acne vulgaris comedones Leeming, J.P.;K.T. Holland;W.J. Cunliffe https://doi.org/10.1099/00222615-20-1-11
  18. Br. J. Dermatol. v.118 The microbial colonization of inflamed acne vulgaris lesions Leeming, J.P.;K.T. Holland;W.J. Cunliffe https://doi.org/10.1111/j.1365-2133.1988.tb01775.x
  19. J. Gen. Microbiol. v.130 The microbial ecology of pilosebaceous units isolated from human skin Leeming, J.P.;K.T. Holland;W.J. Cunliffe
  20. J. Biol. Chem. v.193 Protein measurement with the Folin phenol reagent Lowry, O.H.;N.J. Rosebrough;A.L. Farr;R.J. Randall
  21. Appl. Environ. Microbiol. v.35 Regional variations of cutaneous propionibacteria McGinley, K.J.;G.F. Webster;J.J. Leyden
  22. Bergeys Manual of Determinative Bacteriology Propionibacterium Moore, W.E.;L. Vholdman;Buchanan RE(ed.);Gibbons NE(ed.)
  23. Indian J. Dermotol. v.28 Epidemiology of acne vulgaris Pandey, S.S.
  24. In Berlin Springer (ed 2) Acne Plewig, G.;A.M. Kligman
  25. Arch. Dermatol. v.93 Corynebacterium acnes. Presence of complement fixing antibodies to Corynebacterium acnes in the sera of patients with acne vulgaris Puhvel, S.M.;I.K. Hoffman;T.H. Sterberg https://doi.org/10.1001/archderm.93.3.364
  26. Arch. Dermatol. v.90 Study of antibody levels to Corynebacterium acnes Puhvel, S.M.;M. Barfatani;M. Warnick;T.H. Sternberg https://doi.org/10.1001/archderm.1964.01600040049011
  27. J. Exp. Med. v.145 The adjuvant effect of Corynebacterium parvumn: T-cell dependence of macrophage activation Sljivic, V.S.;S.R. Watson https://doi.org/10.1084/jem.145.1.45
  28. J. Invest. Dermatol v.79 Activation of components of the alternative pathway of complement by Propionibacterium acnes cell wall carbohydrate Webster, G.F.;W.P. McArthur https://doi.org/10.1111/1523-1747.ep12500042
  29. Arch. Dermatol. v.82 The pathologic dynamic of acne vulgaris Strauss, J.S.;A.M. Kligman https://doi.org/10.1001/archderm.1960.01580050121019
  30. Proc. Natl. Acad. Sci. USA v.76 Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications Towbin, H.;T. Staehelin;J. Gordon https://doi.org/10.1073/pnas.76.9.4250
  31. The histopathology of disease of the skin Unna, P.
  32. Infect. Immun. v.63 Induction of proinflammatory cytokinse by a soluble factor of Propionibacterium acnes: Implications for chronic inflammatory acne Vowels, B.R.;S. Yang;J.J. Leyden
  33. Infect. Immun. v.26 Complement activation in acne vulgaris: consumption of complement by comedones Webster, G.F.;J.J. Leyden;U.R. Nilsson
  34. J. Invest. Dermatol. v.74 Polymorphonuclear leukocyte lysosomal release in response to Propionibacterium acnes in vitro and its enhancement by sera from inflammatory acne patients Webster, G.F.;J.J. Leyden;C.C.T. Sai;P. Baehni;W.P. McArthur https://doi.org/10.1111/1523-1747.ep12544494
  35. Infect. Immun. v.22 Complement activation in acne vulgaris: in vitro studies with Propionibacterium acnes and Propionibacterium granulosum Webster, G.F.;J.J. Leyden;U.R. Nilsson;M.E. Norman
  36. Inflammation v.5 Activation of the alternative pathway of complement in human serum by Propionibacterium acnes(Corynebacterium parvum) cell fraction Webster, G.F.;U.R. Nilsson;W.P. McArthur https://doi.org/10.1007/BF00914205
  37. J. Am. Acad. Dermatol. v.39 Recent finding in the epidemiologic evidence, classification, and subtypes of acne vulgaris White, G.M. https://doi.org/10.1016/S0190-9622(98)70442-6
  38. J. Invest. Dermatol. v.60 Incidence and lipolytic activity of Propionibacterium acnes (Cornebacterium acnes group Ⅰ) and Propionibacterium granulosum (C. acnes groupⅡ) in acne and in normal skin White. side J.A.;J.G. Voss https://doi.org/10.1111/1523-1747.ep12724177