Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
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Degradation of Phenanthrene by Trametes versicolor and Its Laccase

  • Han, Mun-Jung (Division of Biological Sciences, Kangwon National University) ;
  • Park, Hyoung-Tae (Division of Biological Sciences, Kangwon National University) ;
  • Song, Hong-Gyu (Division of Biological Sciences, Kangwon National University)
  • Published : 2004.06.01
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Abstract

Phenanthrene is a three-ring polycyclic aromatic hydrocarbon and commonly found as a pollutant in various environments. Degradation of phenanthrene by white rot fungus Trametes versicolor 951022 and its laccase, isolated in Korea, was investigated. After 36 h of incubation, about 46％ and 65％ of 100 mg/l of phenanthrene added in shaken and static fungal cultures were removed, respectively. Phenanthrene degradation was maximal at pH 6 and the optimal temperature for phenanthrene removal was 30$^{\circ}C$. Although the removal percentage of phenanthrene was highest (76.7％) at 10 mg/1 of phenanthrene concentration, the transformation rate was maximal (0.82 mg/h) at 100 mg/L of phenanthrene concentration in the fungal culture. When the purified laccase of T. versicolor 951022 reacted with phenanthrene, phenanthrene was not transformed. The addition of redox mediator, 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) or 1-hydroxybenzotriazole (HBT) to the reac-tion mixture increased oxidation of phenanthrene by laccase about 40％ and 30％, respectively.

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References

  1. Appl. Environ. Microbiol. v.62 Polycyclic aromatic hydrocarbon-degrading capabilities of Phanerochaets laevis HHB-1625 and its extracelluar ligninolytic enzymes Bogan, B.W.;R.T. Lamar
  2. Appl. Environ. Microbiol. v.62 Fluorene oxidation in vivo by Phanerochaete chrysosprium and in vitro during manganese peroxidase-dependent lipid peroxidation Bogan, B.W.;R.T. Lamar;K.E. Hammel
  3. Appl. Environ. Microbiol. v.61 Lignin oxidation by laccase isozymes from Trametes versicolor and role of the mediator 2,2'-azinobis(3-ethybenzthiazoline-6-sulfonate) in kraft lignin depolymerization Bourbonnais, R.;M.G. Paice;I.D. Reid;P. Lantheir;M. Yaguchi
  4. Biodegradation v.3 Biodegradation of polycyclic aromatic hydrocarborns Cerniglia, C.E. https://doi.org/10.1007/BF00129093
  5. Biotechnol. Lett. v.24 Oxidation of polycyclic aromatic hydrocarborns by laccase of Coriolus hirsutus Cho, S.-J.;S. Park;J.-S. Lim;Y. Rhee;K.-S. Shin https://doi.org/10.1023/A:1019896316366
  6. Kor. J. Microbiol. v.35 Biodegradation of pyrene in marine environment Hwang, S.-S.;H.-G. Song
  7. Appl. Environ. Microbiol. v.50 Production of ligninases and degradation of lignin in agitated submerged cultures of phanerochaete chrysosporium Jager,A.;S. Croan;T. Kirk
  8. Appl. Microbiol. Biotechnol. v.46 Degradation of anthracene by laccase of Trametes versicolor in the presence of different mediator compounds Johannes, C.;A. Majcherczyk;A. Huttermann https://doi.org/10.1007/s002530050823
  9. J. Bacteriol. v.182 Biodegradation of high-molecular-weight polycyclic aromatic hydrocarbons by bacteria Kanaly, R.;S. Harayama https://doi.org/10.1128/JB.182.8.2059-2067.2000
  10. J. Microbiol. v.40 Cellular responses of Pseudomonas sp. KK1 to Two-ring polycyclic aromatic hydrocarbon, naphthalene Kang, H.-Y.
  11. Biochem. J. v.268 Comparison of lignin peroxidase, horseradish peroxidase and laccase in the oxidation of methoxybenzenes Kersten, P.J.;B. Kalyananraman;K.E. Hammel;B. Reinhammar
  12. J. Microbiol. Biotechnol. v.8 Degradation of polycyclic aromatic hydrocarbons by selected white-rot fungi and the influence of lignin peroxidase Kim, M.S.;E.J. Huh;H.K. Kim;K.W. Moon
  13. Appl. Microbiol. Biotechnol. v.61 Transformation and mineralization of 2,4,6-trinitrotoluene by the white rot fungus Irpex lacteus Kim,H.-Y.;H.-G. Song
  14. Appl. Environ. Microbiol. v.64 Successive mineralization and detoxification of benzo[a]pyrene by the white rot fungus Bjerkandera sp. Strain BOS55 and indigenous microflora. Kotterman, M.J.J.;E.H. Vis;J.A. Field
  15. Appl. Environ. Microbiol. v.62 Development of fungal inocula for bioaugmentation of contaminated soils Lestan, D.;R.T. Lamar
  16. Int. Biodet. Biodeg. v.52 Degradation of organic pollutants by the white rot basidiomycete Trametes trogii Levin, L.;A. Viale;A. Forchiassin https://doi.org/10.1016/S0964-8305(02)00091-4
  17. Appl. Microbiol. Biotechmol. v.46 Degradation of anthracene by laccase of Trametes versicolor in the presence of different mediator compounds Majcherczyk, A.;C. Johannes;A. Huttermann https://doi.org/10.1007/s002530050823
  18. Enzyme Microb. Biotechnol. v.22 Oxidation of polycyclic aromatic hydrocarbons (PAH) by laccase of Trametes versicolor Majcherczyk, A.;C. Johannes;A. Huttermann https://doi.org/10.1016/S0141-0229(97)00199-3
  19. Biotehchnol. Lett. v.24 Enhanced production of laccase in the fungus Trametes versicolor by the addition of xenobiotics Mougin, C.;A. Kollmann;C. Jolivalt https://doi.org/10.1023/A:1013802713266
  20. Appl. Environ. Microbiol. v.63 Comparison of phenanthrene and pyrene dagradation by different wood-decaying fungi Sack, U.;T.M. Heinze;J. Deck;C.E. Cerniglia;R. Martens;F. Zadrazil;W. Fritsche
  21. Int. Biodet. Biodeg. v.43 Degradation of fluorene, anthracene, phenanthrene, fluoranthene, and pyrene lacks connection to the production of extracellular enzymes by Pleurotus ostreatus and Bjerkandera adusta Schutzendubel, A.;A. Majcherczyk;C. Johannes;A. Huttermann https://doi.org/10.1016/S0964-8305(99)00035-9
  22. J. Microbiol. v.35 Biodegradation of aromatic hydrocarbons by several white-rot fungi Song,H.-G.
  23. Biodegradation of Nitroaromatic Compounds Biodegradation of 2,4,6-trinitrotoluene by the white rot fungus Phanerochaete chrysosporium Stahl, J.D.;S.D. Aust;J. Spain(ed.)
  24. Appl. Environ. Microbiol. v.56 Effect of agitation on ligninase activity and ligninase production by Phanerochaete chrysosporium Venkatadri, R;R. Irvine