Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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High Production of L-Threonine using Controlled Feeding of L-Methionine and Phosphate by Escherichia coli Mutant

L-Methionine과 Phosphate의 제한 공급에 의한 Escherichia coli MT201로부터의 고농도 L-Threonine 생산

  • 이만효 (한국생명공학연구원 산업화지원실) ;
  • 이홍원 (한국생명공학연구원 산업화지원) ;
  • 김병진 (경남대학교 식품생명공학) ;
  • 김천석 (한국생명공학연구원 산업화지원) ;
  • 정준기 (한국생명공학연구원 산업화지원) ;
  • 황용일 (경남대학교 식품생명공학부)
  • Published : 2004.06.01
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Abstract

L-Threonine fermentation process was constructed on batch and fed-batch culture by using Escherichia coli MT201. The production type of L-threonine was observed as growth-associated production in batch culture. In fed-batch culture studying optimal concentration of yeast extract in feeding media, when 600 g/l of glucose and 60 g/l of yeast extract were added in feeding media, 87 g/$\ell$ of L-threonine was produced. To improve cell growth and L-threonine production, the culture of high cell density was performed in fed-batch culture with oxygen enriched air and feeding media containing L-methionine and phosphate. Under the conditions, we could achieve the highest L-threonine production of98 g/$\ell$ at 60 h. The highest productivity of L-threonine was about 3.85 g/$\ell$/h.

븐 연구에서는 E. coli MT201을 이용한 L-threonine 발효공정을 확립하였다. 회분식 배양에서 L-threonine생산이 균체 증식과 더불어 증가하는 증식과 연관된 형태의 생산 곡선을 보여주었다. L-Threonine 생산에 대한 조건을 확립하기 위한 유가식 배양에서 첨가배지내 최적 효모추출물과 포도당의 농도는 각각 60 g/$\ell$와 600 g/$\ell$이였으며, 약 87 g/$\ell$의 L-threonine이 생산되었다. 유가식 배양에서도 L-threonine 생산은 회분식 배양과 마찬가지로 균체량의 증가와 더불어 L-threonine 생산이 증가하는 증식과 연관된 형태를 보여주었다. 이러한 결과를 바탕으로 적절한 균체량의 증가와 L-threonine 생산성 향상을 위하여 L-methionine과 인산염이 추가로 공급된 첨가배지를 이용한 고농도 배양 조건이 확립되었다. 한편 고농도 배양에 따른 용존산소의 결핍을 해결하기 위하여 산소가 포함된 혼합공기를 사용하였다. 최적화된 유가식 배양 결과 균체 증식도 원활하게 증가하였으며, L-threonine의 생산도 약 98 g/$\ell$로 향상되었다. 이때 L-threonine의 생산성은 약 3.85 g/$\ell$/h이었다.

Keywords

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