Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Comparative Effect of Plant Growth Regulators on Callus Induction and Plant Regeneration in Glehnia littoralis Schmidt et Miquiel

갯방풍(Glehnia littoralis Schmidt et Miquiel)에서 캘러스 유도 및 식물체 재분화에 미치는 생장조절제의 효과

  • 이병국 (전북대학교 생물자원과학부, (주)플랜넷) ;
  • 한미숙 (전북대학교 생물자원과학부, (주)플랜넷) ;
  • 정양균 (전주농림고등학교) ;
  • 나의식 (순천대학교 식물생산과학부) ;
  • 윤성중 (전북대학교 생물자원과학부) ;
  • 유남희 ((주)플랜넷)
  • Published : 2004.06.01
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Abstract

Glehnia littoralis is known as an edible and medicinal plant using green loaves and mature roots of plant. In the present paper, the influence of plant growth regulators on callus induction and plant regeneration was investigated. Callus induction and regeneration occurred from leaf and petiole explants in Glehnia littoralis. Optimal condition of plant growth regulators for callus induction from leaf and petiole explants was MS basal medium supplemented with 2mg/L 2,4-D and 2mg/L BA. The frequency of callus induction was higher in petiole explant than leaf. When the callus was cultured on MS basal medium supplemented with 0∼1 mg/L IAA, 0∼1mg/L NAA and 0∼2mg/L BA for about 65 days, the most effective plant growth regulators on plant regeneration from callus were 1mg/L NAA and 2mg/L BA. The plantlets acclimatized successfully and grown in vermiculite matrix.

갯방풍의 캘러스 유도와 식물체 재분화에 영향을 미치는 생장조절제에 대한 몇 가지 요인들을 조사하였다. 식물체 조직으로부터 캘러스의 유도에 적절한 배지의 조건은 1∼2 mg/L, 2,4-D와 1∼2 mg/L BA 혼용처리 조합에서 캘러스의 유도율이 높았고, 조직별 캘러스 유도율은 엽조직 보다 엽병에서 우수하였으나, 다른 식물의 부정아 발생 양상과 달리 엽조직에서 발생한 캘러스로부터 부정아와 부정근의 형성을 보였다. 조직별 캘러스의 식물체 분화율은 식물생장 조절제의 농도가 높을수록 양호하였고 적정농도는 1 mg/L NAA와 2 mg/L BA 이었다. 이후 분화된 식물체를 계대배양하여 대량증식을 위해 multiple shoot를 발생시키고 뿌리를 발생시킬 수 있도록 발근배지에 옮겨 생육 시킨 후 식물체의 초장에 따라 vermiculite에 순화시켜 생존율을 조사한 결과 높은 생존율을 보임을 알 수 있었다.

Keywords

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