International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
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Pheophytin Content and Cytotoxicity of Silkworm Feces Against Jurkat Cells According to Dry Method and Storage Period

  • Ahn, Mi-Young (Department of Agricultural Biology, National Institute of Agricultural Science, & Technology) ;
  • Kim, Iksoo (Department of Agricultural Biology, National Institute of Agricultural Science, & Technology) ;
  • Ryu, Kang-Sun (Department of Agricultural Biology, National Institute of Agricultural Science, & Technology) ;
  • Kim, Jin-Won (Department of Agricultural Biology, National Institute of Agricultural Science, & Technology) ;
  • Lee, Heui-Sam (Department of Agricultural Biology, National Institute of Agricultural Science, & Technology) ;
  • Lee, Pyeong-Jae (Department of Agricultural Biology, National Institute of Agricultural Science, & Technology) ;
  • Ko, Si-Hwan (Department of Microbiology, Yonsei University, College of Medicine) ;
  • Lee, Won-Young (Department of Microbiology, Yonsei University, College of Medicine)
  • Published : 2004.06.01
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Abstract

In order to find potential anticancer agents, we extracted pheophytin from silkworm feces according to various dry and storage methods such as sun dry, shade dry, fresh freezing dry and freezing dry after freezing storage (for 1∼3 years). The pheophytin extracts, mainly 10-hydroxypheophytin a, little b, of various storage silkworm feces were analyzed by reversed-phase high-performance liquid chromatography with photodiode array and fluorescence detection. The content of those pheophytih in old silkworm for 3 years (freezing storage and freezing dried in use, or freezing dried and cold storage) was better than others. The cytotoxicity of the pheophytin extracts and ethanol extracts of various storage silkworm feces were measured using Jurkat cells originated from human leukemia, using dye uptake assay (MTT) in order to find effective photodynamic therapeutic agents. The anticancer activity of those pheophytin extracts in various storage methods showed little difference among them. But ethanol extracts of fresh freezing dried silkworm in the current year was good cytotoxic activity than those of any other silkworm feces. With regards to these results, fresh ethanol extracts of silkworm feces were better than old ones. On the other hands, the pheophytin extracts of old silkworm feces contained the highest percentage of pheophytin content and showed good cytotoxicity against cancer cells by changing the pheophytin into pheophobide in the degradative process.

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References

  1. Ahn, M. Y., K. S. Ryu, I. Kim, S. Y. Kim. H. S. Lee, J. W. Kim and Y. K. Lee (2001) Cytotoxicity of the ethanol extracts of mulberry leaves, branches and silkworm feces. Korean J. Seric. Sci. 43, 26-28
  2. Ahn, M. Y., K. S. Ryu, J. W. Kim, I. Kim, H. S. Lee, Y. K. Lee and E. S. Kim (2002) Pheophytin content and fibrinolytic Activity of silkworm feces in the different larval stages of silkworms. Int. J. Indust. Entomol. 5, 195-199
  3. Almela, L., J. A. Fernandez-Lopez and M. J. Roca (2000) High-performance liquid chromatographic screening of chlorophy11 derivatives produced during fruit storage, J. Chromatograpy A 870, 483-489 https://doi.org/10.1016/S0021-9673(99)00999-1
  4. Braumann, T. and L. H. Grimme(1981) Reversed-phase high performance liquid chromatography of chlorophylls and carotenoids, Biochim. Biophys. Acta. 637, 8-17 https://doi.org/10.1016/0005-2728(81)90204-8
  5. Carmichael, J., W. G. Degrasff, A. F. Gazdar, J. D. Minna and J. B. Mitchell (1987) Evaluation of tetrazolium-based semiautomated colorimetric assay: assessment of chemosensitivity testing. Cancer Res. 47, 936-942
  6. Cui, Z., M. Y. Ahn, Y. B. Lee and K. S. Ryu (2002) Materia medica from insects. pp. 150, Shinilbooks, Seoul
  7. Kim, I., H. S. Lee, J. W. Kim, B. K. Yang, M. Y. Ahn, D. H. Kim and K. S. Ryu (2001) Variation of mineral compositions in the regional, varietal, and seasonal mulberry leaves, Int. J. Indust. Entomol. 2, 27-35
  8. Lee, W. Y., J. H. Park, B. S. Kim, M. J. Han and B. S. Hahn (1990) Chlorophy11 derivatives (CpD) extracted from silkworm excreta especially cytotoxic to tumor cells in vitro, Yonsei Med. J. 31, 225-233
  9. Lim, D. S., S. H. Ko, D. H. Won, C. H. Lee and W. Y. Lee (2003) Photodynamic anti-tumor activity of a new chlorinbased photosensitizer against Lewis Lung Carcinoma cells in vitro and in vivo, J. Porphyrins Phthalocyanines 7,155-161 https://doi.org/10.1142/S1088424603000215
  10. Williams, K., R. L. Neice, D. Aterton, A. V. Fowler, K. Kutny and A. J. Smith (1998) The size, operation and technical capabilities of protein and nucleic acid core facilities. FASEB J. 2, 3124-30