Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Effect of Amino Acid Solution for Cell Growth and MPS Activity of Mineral Phosphate Microorganisms.

아미노산액 처리에 의한 인산가용화균주의 생육 및 가용화 효과

  • 이진우 (동아대학교 응용생명공학부) ;
  • 정연주 (동아대학교 응용생명공학부) ;
  • 최시림 (동아대학교 응용생명공학부) ;
  • 김재훈 (동아대학교 응용생명공학부) ;
  • 유주순 (동아대학교 응용생명공학부) ;
  • 김영길 (동아대학교 식품과학부) ;
  • 최용락 (동아대학교 응용생명공학부)
  • Published : 2004.06.01
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Abstract

Phosphate-solubilizing activities of the two strains (Burkholderia sp. DA23 and Klebsiella sp. DA7l-1) against tri-calcium phosphate and hydroxyapatite were quantitatively determined. Two strains were found to solubilize two types of insoluble phosphate different amounts of amino acid solutions in liquid culture. MPS ability of the strains was increased with concentration of amino acid addition. The optimal solubilization condition of insoluble phosphate in sucrose minimal medium were 0.1% amino acid solution, respectively. The efficiency of amino acid addition was no difference between the two types of insoluble phosphate, tri-calcium phosphate and hydroxyapatite.

인산가용화능이 우수한 균주인 Burkholderia sp. DA23과 Klebsiella sp. DA7l-1 두 균주를 사용하여 농도별 아미노산 첨가에 따른 성장 양상과 유리인산 방출량을 조사한 결과, 두 균주의 생육과 인산가용화에 있어 아미노산이 도움이 되는 것으로 나타났다. DA23과 DA7l-1 모두 0.05%와 0.1%의 아미노산을 SM배지에 첨가하여 성장 양상을 확인한 결과 아미노산을 첨가한 배지의 균주가 아미노산을 처리하지 않은 배지의 균주에 비해 높은 세포 밀도 성장을 보여주었다. 인산가용화 균주의 아미노산 첨가의 경우 무처리보다 높은 pH 저하를 보였으며, 또한 아미노산 첨가가 아미노산 무처리구에 비해 2배이상 높은 인산가용화 효과를 보였다. DA7l-1의 아미노산 첨가의 경우는 DA23의 아미노산 첨가보다 아미노산 첨가에 의한 인산가용화 효과가 더 있는 것으로 나타났다. hydroxyapatite와 tri-calcium phosphate 의 인산염 차이에 따른 아미노산 처리 효과는 없었으며, 0.05%의 미량의 농도에서도 인산가용화로의 세포밀도 성장 및 가용화능에 효과를 나타내었다.

Keywords

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