Journal of the Korean institute of surface engineering (한국표면공학회지)

The Korean Institute of Surface Engineering (한국표면공학회)
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Effect of Solvent on the Surface of Protein Chip Plate

단백질 칩 기판의 표면에 미치는 용매 효과

  • Published : 2004.04.01
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Abstract

Nickel chloride coated protein chip plate was developed by using a spin coating method. The ability of histidine tagged protein adsorption was investigated at various solvents. The surface of plate has a large aggregated nickel complex with high density in water. However, the surface of plate has a very small size of aggregated nickel complex with low density in isopropanol. The ability of protein adsorption decreased as increasing the size of alkyl chain in various alcohol solvents. The mechanism on the ability of protein adsorption at the plate surface is discussed.

Keywords

References

  1. E. T. Fung, V. Thulasiraman, S. R. Weinberger, E. Dalmasso, Protein Biochips for Differential Profiling. Current Opinion in Biotechnology, 12 (2001) 65 https://doi.org/10.1016/S0958-1669(00)00167-1
  2. G. MacBeath, S. Schreiber, Printing Proteins as Microarrays for High-throughput Function Determina-tion. Science, 289 (2000) 1760
  3. D. V. Nicolau, H. Taguchi, H. Taniguchi, S. Yoshikawa, Micron-sized Protein Patterning on Diazonaphthoquinone /novolak Thin Polymeric Films. Langmuir, 14 (1998) 1927 https://doi.org/10.1021/la970802g
  4. R. Bashir, R. Gomez, A. Sarikaya, M. R. Ladisch, J. Sturgis, J. P. Robinson, Adsorption of Avidin on Microfabricated Surfaces for Protein Biochip Applications. Biotechnol. Bioeng., 73 (2001) 324 https://doi.org/10.1002/bit.1065
  5. J. W. Hyun, S. Y. Kim, S. Lee, H. Park, J. Pyee, S. Kim, Bull. Korean Chem. Soc., 23 (2002) 1725