Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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A New Thermophile Strain of Geobacillus thermodenitrificans Having L- Arabinose Isomerase Activity for Tagatose Production

  • Baek, Dae-Heoun (Department of Bioscience and Biotechnology, Sejong University) ;
  • Lee, Yu-Jin (Department of Bioscience and Biotechnology, Sejong University) ;
  • Sin, Hong-Sig (Department of Bioscience and Biotechnology, Sejong University) ;
  • Oh, Deok-Kun (Department of Bioscience and Biotechnology, Sejong University)
  • Published : 2004.04.01
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Abstract

Five strains, producing bacterial thermostable L-arabinose isomerase, were isolated from Korean soil samples obtained from compost under high temperature circumstances. Among these strains, the CBG-Al showed the highest L-arabinose isomerase activity at $60^\circ{C}$ and was selected as a D-tagatose producing strain from D-galactose. This strain was identified as Geobacillus thermodenitrificans based on the 16S rRNA analysis, and biological and biochemical characteristics. The isolated strain was aerobic, rod-shaped, Gram-positive, nonmotile, and an endospore-forming bacterium. No growth was detected in culture temperature below $40^\circ{C}$. The maximum growth temperature and maximum temperature of enzyme activity were $75^\circ{C}$ and $65^\circ{C}$, respectively. In metal ion effects, $Ca^{2+}$ was the most effective enzyme activator with the reaction rate by 150%. In a 5-1 jar fermentor with 3-1 MY medium, L-arabinose isomerase activity was growth-associated and pH decreased rapidly after the initial logarithmic phase.

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