Plant Resources
- 제7권1호
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- Pages.39-46
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- 2004
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- 1226-8771(pISSN)
Molecular and Cytogenetic Analysis of Transgenic Plants of Rice(Oryza sativa L.) Produced by Agrobacterium-mediated Transformation
- Cho, Joon-Hyeong (Division of Environment and Biotechnology, National Institute of Crop Science, Rural Development Adminstration) ;
- Kim, Yong-Wook (Department of Plant Resources, Dongguk University)
- 발행 : 2004.04.01
초록
To demonstrate the importance of transformation efficiency in independent event, molecular and cytogenetic analysis were conducted with genomic DNA and chromosome of transgenic plants produced by Agrobacterium tumefeciens LBA4404 (pSBM-PPGN: gusA and bar). Selection ratios of putative transgenic calli were similar in independent experiments, however, transformation efficiencies were critically influenced by the type of regeneration media. MSRK5SS-Pr regeneration mediun, which contains 5 mgL
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