Insecticidal Activity of Bacillus thuringiensis 656-3 Strain to Mushroom Flies in Oyster Mushroom House

  • Park, Yong-Soo (College of Natural Resources and Life Science, Dong-A University) ;
  • Kang, Young-Jin (Department of Pharmacology, College of Medicine, Gyeongsang National University, College of Natural Resources and Life Science, Dong-A University) ;
  • Lee, Kwang-Sik (College of Natural Resources and Life Science, Dong-A University) ;
  • Jianhong Le (College of Plant Science and Technology, Huazhong Agricultural University) ;
  • Je, Yeon-Ho (School of Agricultural Biotechnology, Seoul National University) ;
  • Roh, Jong-Yul (School of Agricultural Biotechnology, Seoul National University) ;
  • Seo, Sook-Jae (Division of Life Science, College of Natural Sciences, Gyeongsang National University) ;
  • Sohn, Hung-Dae (College of Natural Resources and Life Science, Dong-A University) ;
  • Jin, Byung-Rae (College of Natural Resources and Life Science, Dong-A University)
  • Published : 2004.03.01

Abstract

Bacillus thuringiensis 656-3 which was isolated from a soil sample of mushroom house and showed high toxicity to mushroom flies, Lycoliella mali and Coboldia fuscipes, was surveyed for insecticidal effect in the oyster mushroom house.3. thutingiensis 656-3 was mass-cultured in the fermenter containing soybean cake(2%) and wheat bran (2%) as media source. Semi-for-mutation of B. thuringiensis 656-3 was performed with metamorphic starch only. When the formulation suspension containing $5{\times}{10^7}$ cfu was sprayed on the mushroom in mushroom house, the insecticidal effect of B. thuringiensis 656-3 to mushroom flies,1. mali and C. fuscipes, was maintained over 90% by the fifth day after starting spraying. The yield of oyster mushroom house with B. thuringiensis 656-3 was significantly increased compared to control. B. thuringiensis 656-3 represents a powerful biological insecticide for the control of mushroom flies.

Keywords

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