International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
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Scanning Electron Microscopic Studies on Leaf Surface Trichomes in Mulberry and Its Influence on Rearing Performance of Silkworm Bombyx mori L.

  • Kesavacharyulu, K. (Mulberry Breeding and Genetics Laboratory, Central Sericultural Research and Training Institute) ;
  • Kumar, Vineet (Electron Microscopy Unit, Central Sericultural Research and Training Institute) ;
  • Sarkar, A. (Mulberry Breeding and Genetics Laboratory, Central Sericultural Research and Training Institute)
  • Published : 2004.03.01
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Abstract

The type of trichomes, their density and pattern of distribution on leaves of 16 genotypes of mulberry, belonging to both diploid and polyploid categories, were studied by scanning electron microscope. The present investigation was undertaken to find out the relationship of physical attributes, especially the density and trichome types with higher acceptability and better rearing performance by the silkworm Bombyx-mori L. Two types of trichomes glandular and non-glandular types were observed on both the leaf surfaces of all the mulberry genotypes studied. In general, greater densities of trichomes were observed on the abaxial surface than the adaxial surface of leaves in most of the genotypes. Distribution of glandular trichomes were more in abaxial surface and non-glandular trichomes were more in adaxial surface. Overall, distribution of glandular and non-glandular trichomes per unit area of leaf did not follow any regular pattern. When leaves of those genotypes were fed to silkworms, trichome density was found to be significantly negatively correlated with the survival of larvae i.e., effective rate of rearing, but trichome density did not influence the economic characters of rearing. As the distribution of glandular trichomes (GT) and non-glandular trichomes (NGT) did not follow any definite pattern, no relation could be established between the GT and NGT densities with silkworm rearing performance. However, the ratio of GT and NGT in a particular genotype influenced the rearing parameters, higher the ratios better the rearing performance. High GT and NGT ratio (＞1.00) was found positively significant when correlated with economic parameters viz., larval weight, single cocoon weight and single shell weight. The study is useful in screening different mulberry genotypes for their better acceptability to silk-worm and higher rearing performance at the early stage of selection without actually conducting the rearing.

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References

  1. Al Jibouri, H. A., P. A. Miller and H. P.Robinson(1958) Genotypic and environmental Variances in upland cotton of inter specific origin. Agron. J. 50, 633-637 https://doi.org/10.2134/agronj1958.00021962005000100020x
  2. Ascensao, L., N. Marques and M. S. Pais (1995) Glandular trichomes on vegetative and Reproductive organs of Leonotis leonurus (Lamiaceae). Ann. Bot. 75, 619-626 https://doi.org/10.1006/anbo.1995.1067
  3. Ascensao, L., L. Mota and M. De M. Castro (1999) Glandular trichomeson the leaves and flowers of Plectranthus omatus: Morphology, distribution and histochemistry. Ann. Bot. 84, 437-447 https://doi.org/10.1006/anbo.1999.0937
  4. Beck, S. D. (1965) Resistance of plants to insects. Annu. Rev. Entoml. 10, 207-232 https://doi.org/10.1146/annurev.en.10.010165.001231
  5. Callahan, P. S. (1957) Oviposition response of the com earworm to differences in surface texture. J. Kans. Entomol. Soc. 30, 59-63
  6. Fujita, H. and C. Uchikawa (1986) Electron microcopical study of mulberry with special Reference to the identification of cultivars; in Development of new technology for identification and classification of tree crops and ornamentals. Kitaura, K., M. Horie and I. Kozaki. (eds.), pp. 25-29. Fruit Tree Research Station, Ministry of Agriculture, Forestry and Fisheries, Japan
  7. Jermy, T. (1984) Evolution of insect/host plant relationships. Am. Nat. 124, 609-630 https://doi.org/10.1086/284302
  8. Katsumata, F. (1971) Shape of idioblasts in mulberry leaves with special reference to the classification of mulberry trees. J. Sericult. Sci. Jpn 40, 313-322
  9. Katsumata, F. (1972) Relationship between the length of style and the shape of idioblasts in mulberry leaves, with special reference to the classification of mulberry trees. J. Sericul. Sci. Jpn 41,387-395
  10. Kawakami, K. (2001) Illustrated working process of new bivoltine silkworm rearing technology. Published by JICA, PPPBST project, CSRTI, Mysore. pp. 87
  11. Levin, D. A. (1973) The role of trichomes in plant defence. Q. Rev. BioI. 48, 3-15 https://doi.org/10.1086/407484
  12. Mehta, I. J., P. S. Dhillon and G. P. Hanson (1979) Trichome morphology as an indicator of high rubber bearing guayule (Parthenium argentatum Gray) Plants in native populations. Am. J. Bot. 66, 769-804
  13. Metcalf, C. R. and L. Chalk (1979) Anatomy of the dicotyledons. 2nd ed. Vol. I. Systematic anatomy of leaf and stem, with a brief history of the subject. Clarendon Press. Oxford
  14. Norris, D. M. and M. Kogan (1980) Biochemical and morphological bases of resistance; in Breeding plants resistant to insects. Maxwell, F. G. and P. R. Jennings (eds.), pp. 23-62, John Wiley & Sons, New York
  15. Pedro, L. G., J. G. Barroso, N. T. Marques, L. As$\^c$ensao, M. S. Pais and J. J. Scheffer (1991) Composition of essential oil from sepals of Leonotis leonurus R. Br. J. Ess. Oil Res. 3, 451-453 https://doi.org/10.1080/10412905.1991.9697985
  16. Purushothaman, K. K. and S. Vasanth (1988) Chemical studies on Leonotis a review. Indian Drugs 25, 484-491
  17. Singh, B. B., H. H. Hardley and R. L. Bernard (1971) Morphology of pubescence in Soybeans and its relationship to plant vigour. Crop Sci. 11, 13-16 https://doi.org/10.2135/cropsci1971.0011183X001100010004x
  18. Werker, E. (1993) Function of essential oil secreting glandular hairs in aromatic plants of Lamiaceae- a review. Flav. Frag. J. 8, 249-255 https://doi.org/10.1002/ffj.2730080503
  19. William, W. T. and P. I. Healey (1984) Cellular basis of trichome secretion; in Biology and chemistry of plant trichomes. Rodriguez, B., P. I. Healey and I. Mehata (eds.), pp. 95-111, Plenum Press. New Yark