Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Controlling the Gene Expression of Corynebacterium diphtheria Toxin-A Using the Tet-On System in Mouse Embryonic Stem Cells.

Mouse Embryonic Stem Cell에서 Tetracycline-Inducible System(Tet-on System)을 이용한 Corynebacterium diphtheria Toxin-A유전자의 발현 조절

  • 박재균 (포천중문의과대학교 세포유전자치료연구소) ;
  • 임수빈 (포천중문의과대학교 세포유전자치료연구) ;
  • 송지환 (포천중문의과대학교 세포유전자치료연구소)
  • Published : 2004.03.01
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Abstract

Embryonic stem (ES) cells are derived from the inner cell mass of the blastocyst-stage embryos that can be propagated indefinitely and, at the same time, can be differentiated into all the cell types that constitute the body. Current research using ES cells is mainly focused on the efficient generation of specific cell types by employing optimal differentiation conditions, which often requires the genetic manipulation of ES cells. As a way of developing an efficient system to regulate foreign gene expression in ES cells, we have inserted the gene encoding Corynebacterium diphtheria toxin-A (DTA) into an autonomously induced plasmid under positive doxycycline control ('Tet-on' system). In this study, we demonstrate that this system can lead to the cell death of mouse ES cells by the induction of DTA expression when exposed to the tetracycline derivative, doxycycline. MTT assay showed that this induction resulted in the apoptosis of ES cells.

본 연구에서는 C. diphtheriae toxin-A(DTA)를 합성하는 유전자를 tetracycline derivative인 doxycycline에 의해 발현이 유도되는 plasmid('Tet-on' system)에 삽입시켜, 이를 mouse ES cell에 도입시켰으며, 이렇게 제작된 mouse ES cell이 doxycycline의 처리 농도에 따라 mouse ES cell내의 DTA의 발현이 유도되어 이 결과 세포 사별(apoptosis)을 유발시키는 것을 MTT assay를 통해 확인하였다.

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