식이 단백질의 종류와 함황아미노산 함량이 성장기 쥐의 골밀도에 미치는 영향

The Effect of Dietary Protein Source and Sulfur Amino acid Content on bone Metabolism in Growing Rats

  • 최미자 (계명대학교 식품영양학과) ;
  • 정소형 (계명대학교 식품영양학과)
  • 발행 : 2004.03.01

초록

This study was performed to evaluate the effect of dietary protein source and sulfur amino acid content on bone metabolism in ra. Thirty male rats (body weight 145$\pm$2g) were divided into three groups. The rats in the first group were fed on casein 20% diet as animal protein source and those in the second group were fed on soy 20% diet as plant protein source. Sulfur amino acid ratio of these group was 1.07:1. The rats in the third group were fed on soy 20% diet and the sulfur amino acid were supplemented with the amount contained as much in the soy 20% diet. All rats were fed on experimental diet and deionized water ad libitum for 9 weeks, The total body, spine, femur bone mineral density and bone mineral content were measured using Dual Energy X-ray Absorptiometry Calcium, phosphate, pyridinoline, creatinine in urine and calcium, phosphate, alkaline phosphatase, osteocalcin in serum were measured. During the experimental period, plant protein (soy protein) group had a lower urinary Ca excretion, urine pyridinoline & crosslinks value and had a higher Ca efficiency in total bone and femur bone mineral density than animal protein (casein) group. There were no significant differences in serum calcium, phosphate, alkaline phosphatase and osteocalcin among the three groups of the rats. The findings from this study demonstrated that plant protein (soy protein) is beneficial of bone mineral density because it had a higher Ca efficiency in total bone and femur bone mineral density than animal protein (casein). However, the supplementation of sulfur amino acid on soy results were consistent with prior studies that dietary sulfur amino acid load had a negative effect on calcium balance. The rats fed sulfur amino acid supplementation diet increased urinary calcium excretion and decreased calcium efficiency for total and femur mineral density. Therefore, dietary protein source and sulfur amino acid content influence bone metabolism. (Korean J Nutrition 37(2): 100-107, 2004)

키워드

참고문헌

  1. Am J Clin Nutr v.78 no.3 Dietary phytoestrogens and their effect on bone; evidence from in vitro and in vivo, human observational, and dietary intervention studies Setchell,K.D.R.;Lydeking-Olsen E
  2. Am J Clin Nutr v.58 Dietary intakes and urinary excretion of calcium and acids; a cross-sectional study of women in China Hu,J.F.;Zhao,X.H.;Parpia,B.;Campbell,T.C.
  3. N Engl J Med v.330 Improved mineral balance and skeletal metabolism in postmeno-pausal women treated with potassium bicarbonate Sebastian,A.;Harris,S.T.;Ottaway,J.H.;Todd,K.M.;Morris,R.C.Jr. https://doi.org/10.1056/NEJM199406233302502
  4. Am J Clin Nutr v.69 Prospective study of dietary protein intake and risk of hip fracture in postmenopausal women Munger,R.G.;Cerhan,J.R.;Chiu,B.C.
  5. J Nutr v.128 Excess dietary protein can adversely affect bone Barzel,U.S.;Massey,L.K.
  6. Am J Clin Nutr v.68 Estimation of net endogenous noncarbonic acid production in humans from diet potassium and protein contents Frasseto,L.A.;Todd,K.M.;Morris,R.C.Jr.;Sebastian,A.
  7. J Nutr v.111 Role of the sulfur-containing amino acids in protein-induced hypercal-ciuria in men Schuette,S.A.;Zemel,M.B.;Heqsted,M.;Linkswiler,H.M.
  8. Am J Clin Nutr v.33 A comparison of the effects of feeding sulfur amino acide and protein on urine calcium in man Block,G.D.;Wood,R.J.;Allen,L.H.
  9. Am J Epidemiol v.155 Protein consumption and bone minceral density in the elderly; the Rancho Bermardo Study Promislow,J.H.;Goodman-Gruen D.;Slymen,D.J.;Barrett-Connor E. https://doi.org/10.1093/aje/155.7.636
  10. J Nutr v.111 Renal acid, urinary cyclic A.M.P, and hydroxyproline excretion as affected by level of protein sulfur amino acid and phosphorus intake Sally,A.;Schuette,M.H.;Michel,B.Z.;Linkswiler,H.M.
  11. Am J Physiol v.271 Effect of age on blood acid-base composition in adult humans; role of age-related renal functional decline Frassetto,L.A.;Morris,R.C.Jr.;Sebastian,A.
  12. Am J Clin Nutr v.70 no.3 Legumes and soybeans; overview of their nutritional profiles and health effects Messina,M.J.
  13. J Nutr v.44 Nutritional improvement of white polished rice by addition of lysine and threonine Pecora,L.J.
  14. J Nutr Sci Vitaminol v.36 Effects of high protein diet on bone formation and calcium metabolism in rats Funaba,M.;Kawashima,T.;Yano,H.;Kawashima,R. https://doi.org/10.3177/jnsv.36.559
  15. J Nutr v.112 Effect of protein-induced calciuria on calcium metabolism and bone status in adult rats Calvo,M.S.;Bell,R.R.;Forbes,R.M.
  16. J Nutr v.110 The role of sulfate in the calciuria of high protein diet in adult rats Whiting,S.J.;Draper,H.H.
  17. Am J Physiol v.262 Acidosis inhibits osteoblastic and stimulates osteoclastic activity in vitro Krieger,N.S.;Sessler,N.E.;Bushinsky,D.A.
  18. Bone v.18 Modulation of the resorptive activity of rat osteoclasts by small changes in extracellular pH near the physiological range Amett,T.R.;Spowage,M. https://doi.org/10.1016/8756-3282(95)00486-6
  19. Bone and Mineral Supplement 1 v.17 Urinary excretion of pyridinoline; Circadian rhythm Fincato,G.;Leonardis de V;Abbiati,G.;Brandi,M.L.;Bartucci,F.
  20. J Nutr v.127 Phytoestrogens reduce bone loss and bone resorption in ophorectomized rats Draper,C.R.;Edel,M.J.;Dick,I.M.;Randall,A.G.;Martin,G.B.;Raince,R.L.
  21. Bone and Mineral Supplement 1 v.17 Urinary excretion of pyridinium crosslinks during menopause studied longitudinally Hassager,C.;Colwell,A.;Assiri,A.M.A.;Eastell,R.;Russll,R.G.G.;Schlemmer,A.;Christiansen,C. https://doi.org/10.1016/0169-6009(92)92335-N
  22. Bone and Mineral Supplement 1 v.17 Bone mineral density and biochemical markers in postmeno-pausal osteoporosis Negoro,Y.;Tomita,A.;Watanabe,K.;Masahashi,T.;Nakanishi,M.
  23. Am J Clin Nutr v.73 A high ratio of dietary animal to vegetable protein increases the rate of bone loss and the risk of fracture in postmenopausal women Sellmeyer,D.E.;Stone,K.L.;Sebastian,A.;Cummings,S.R.
  24. Korean J Nutrition v.36 no.5 Effects of soy and isoflavones on bone marks and hormones in growing male rats Choi,M.J.
  25. N Engl J Med v.327 Calcium intake and peak bone mass Matkovic,V. https://doi.org/10.1056/NEJM199207093270210
  26. Am J Clin Nutr v.42 The relationship between diet and bone mineral content of multiple skeletal sites in elderly Japanese American men and women living in Hawaii Yano,K.;Heibrun,L.K.;Wasnich,J.H.;Vogel,J.M.
  27. Calcif Tissue Int v.50 Cross-cultural association between dietary animal protein and hip fracture; a hypothesis Abelow,B.;Holford,T.;Insogna,K. https://doi.org/10.1007/BF00297291
  28. Am J Epidemiol v.143 Protein consumption and bone fractures in women Feskanich,D.;Willett,W.C.;Stampfer,M.J.;Colditz,G.A. https://doi.org/10.1093/oxfordjournals.aje.a008767
  29. Am J Epidemiol v.145 Dietary factors and the incidence of hip fracture in middle-aged Norwegian; a prospective study Meyer,H.E.;Pedersen,J.I.;Loken,E.B.;Tverdal,A. https://doi.org/10.1093/oxfordjournals.aje.a009082
  30. J Gerontol A Biol Sci Med Sci v.55 Worldwide incidence of hip fracture in elderly women; relation to consumption of animal and vegetable foods Frassetto,L.A.;Todd,K.M.;Morris,R.C.Jr.;Sebastian,A. https://doi.org/10.1093/gerona/55.10.M585
  31. Am J Clin Nutr v.72 A threshold for low protein diet induced elevations in parathyroid hormone Kerstetter,J.E.;Svastisalee,C.M.;Caseria,D.M.;Mitnick,M.E.
  32. Korean J Nutrition v.36 Effects of soy protein and isoflavones on bone mineral density in growing female rats Choi,M.J.;Jo,H.J.