곤충병원성 선충을 이용한 잔디밤나방, Spodoptera depravata(Butler)(나비목: 밤나방과)의 생물적 방제

Biological Control of Spodoptera depravata (Butler) (Lepidoptera: Noctuidae) Using Entomopathogenic Nematodes

  • 강영진 (경상대학교 의과대학 약리학교실) ;
  • 이동운 (상주대학교 농업과학연구소) ;
  • 추호렬 (경상대학교 응용생명과학부, 농업생명과학원, 환경생명과학 국가핵심연구센터) ;
  • 이상명 (국립산림과학원 남부산림연구소) ;
  • 권태웅 (동래베네스트골프장) ;
  • 신홍균 (삼성에버랜드 잔디, 환경연구소)
  • 발행 : 2004.03.01

초록

우리나라산 곤충병원성 선충(Heterorhabditis sp. Gyeongsan, Steinernema carpocapsae GSN1, S. glaseri Dongrae, S. longicaudum Nonsan, S. monticolum Jiri)을 이용한 잔디밤나방(Spodotera depravata)의 환경 친화적 방제 가능성을 알아보기 위하여 실내와 야외에서 실험을 수행하였다. 아울러 잔디의 예고가 곤충병원성 선충의 효과에 미치는 영향과 야외에서 곤충병원성 선충의 처리가 잔디밭에 분포하고 있는 개미와 집게벌레의 밀도 변화에 미치는 영향도 조사하였다. 잔디밤나방 3령충은 곤충병원성 선충에 대하여 처리 5일째 53.3%에서 66.7%의 보정사충율을 나타내었다. S. carpocapsae GSN1과 S. monticolum Jiri의 잔디밤나방 3령충에 대한 반수치사농도 (LC$_{50}$)는 6.9와 3.9마리였다. S. carpocapsae GSN1의 병원성은 잔디밤나방의 령기에 따라 차이가 있어 2령충에서 73.3%로 가장 높았으며, 3령충부터 감소하였다. 예고는 곤충병원성 선충의 병원성에 영향을 미쳤다. 들잔디의 예고가 14mm인 pot에서 4령충의 치사율은 S. carpocapsae GSN1 처리가 40.0%였고, S. monticolum Jiri처리는 33.3%였으며 fenitrothion은 83.3%의 치사율을 나타내었으나 45mm구에서는 모두 낮았다. 곰개미(Formica japonica) 분포지에서 선충처리구의 보정사충율은 fenitrothion 처리구에 비하여 낮았으나, 개미의 감소율은 fenitrothion구에서 56.7%로 높았고, S. carpocapsae GSN1 처리구와 S. monticolum Jiri 처리구에서는 각각 0%, 6.7%로 낮았다. 잔디의 예고는 S. carpocapsae GSN1과 fenitrothion의 효과에 영향을 미쳤다. S. carpocapsae GSN1은 3-4cm 예고구에서는 62.2%의 방제효과를 나타내였으나, 6-8cm와 12-14cm의 예고구에서는 전혀 효과가 없었다. 그러나 fenitrothion 처리구에서는 유의적 차이가 없었다. 못뽑기집게벌레(Forficula scudderi)의 밀도도 fenitrothion 처리구에서만 감소를 보였다. 즉, 3-4cm 예고구에서는 100%의 감소율을 보였고, 6-8cm 구에서는 41.7%, 12-14cm 구에서는 16.1%의 감소율을 나타내었다.

Environmentally sound control of Japanese lawngrass cutworm, Spodoptera depravata with Korean entomopathogenic nematodes (Heterorhabditis sp. Gyeongsan, Steinernema carpocapsae GSNI , S. glaseri Dongrae, S. longicaudum Nonsan, and S. monticolum Jiri) was evaluated in the laboratory and field. In addition, effect of turf height on the pathogenicity of entomopathogenic nematodes and density of ant (Formica japonica) and spine-tailed earwigs (Forficula scudderi) were investigated in the field. The corrected mortality of 3rd instar of S. depravata larvae was 53.3% to 66.7% 5 days later after treatment of entomopathogenic nematodes. $LC_{50}$ value of S. carpocapsae GSN1 and S. monticolum Jiri against 3rd instar of S. depravata was 6.9 and 3.9, respectively. Pathogenicity of S. carpocapsae GSN1 was different depending on larval stages, i.e., mortality of 2nd instar was the highest representing 73.3% but decreased from 3rd instar. Turf height affected pathogenicity of entomopathogenic nematodes. Mortality of 4th instar of S. depravata was 40.0% by S. carpocapsae GSN1 and 33.3% by S. monticolum Jiri, and 83.3% by fenitrothion, respectively, in the turf height of 14mm, but those were lower in 45mm. The corrected mortality of S. depravata larvae was lower at the entomopathgenic nematode plots than fenitrothion plot in the distribution field of Formica japonica. However, reduction rate of F japonica was higher in fenitrothion by 56.7% compared with 0% in S. carpocapsae GSN1 and 6.7% in S. monticolum Jiri. Turf height influenced control of S. depravate in S. carpocapsae GSN1 and fenitrothion. Control value of S. carpocapsae GSN1 was 62.2% in the turf height of 3-4cm, but not effective at all in 6-8cm and 12-14cm. However, there was not significantly different in fenitrothion plots. Forficula Scudderi was also reduced only in fenitrothion plots. Reduction rate was 100% in 3-4cm, 41.7% in 6-8cm, and 16.7% in 12-14cm, respectively.

키워드

참고문헌

  1. Anonymous. 2003. Manual of pesticides use in Korea. 951 pp. Korean Agricultural Chemicals Industrial Association
  2. Cho, I.H. 1996. Practice and application of SAS. 665pp. Sungandang Pub. Co. Seoul
  3. Choo, H.Y., D.W. Lee, S.M. Lee, T.W. Lee, W.G. Choi, Y.K. Chung and Y.T. Sung. 2000. Turfgrass insect pests and natural enemies in golf courses. Korean J. Appl. Entomol. 39: 171-179
  4. Choo, H.Y., H.K. Kaya and D.K. Reed. 1988. Biological control of onion maggot and tobacco cutworm with insect-parasitic nematodes, Steinernema feltiae and Heterorhabditis heliothidis. Korean J. Appl. Entomol. 27: 185-189
  5. Choo, H.Y., H.K. Kaya and D.W. Lee. 1997. Entomopathogenic nematodes: their potential for biological control of turfgrass insects. Proc. Int. Symp. Biological Control of Insect Pests. pp. 144-159
  6. Choo, H.Y., H.K. Kaya and S.P. Stock. 1995. Isolation of entomopathogenic nematodes (Steinernematidae and Heterorhabditidae) from Korea. Japanese J. Nematol. 25: 44-51
  7. Choo. H.Y., H.H. Kim, D.W. Lee, S.M. Lee, S.H. Park, Y.M. Choo and J.K. Kim. 2001. Practical utilization of entomopathogenie nematoges, S. carpocapsae Pochen strain and Heterorhabditis bacteriophora Hamyang strain for control of chestnut insect pests. Korean J. Appl. Entomol. 40: 69-76
  8. Cockfield, S.D. and D.A. Potter. 1985. Predation on sob webworm (Lepidoptera: Pyralidae) eggs as affected by chlorpyrifos application to Kentucky bluegrass turf. J. Econ. Entomol. 77: 1542-1544
  9. Dutky, S.R., J.V. Thompson and G.E. Cantwell. 1964. A technique for the mass production of the DD-136 nematode. J. Insect Pathol. 6: 417-422
  10. Fuxa, J.R., A.R. Richter and F. agudelo-silva. 1988. Effect of host age and nematode strain on susceptibility of Spodoptera frueiperda to Steinernema feltiae. J. of Nematol. 20: 91-95
  11. Guo, P.L., D.L. Zhang and X.P. Zhang. 1993. Observation on the biological characters of Sidemia depravata (Butler). Entomological Knowledge 30: 103-106
  12. Hatsukade, M. 1994. Control of insect pests with entomopathogenie nematodes. II. control of turfgrass insect pests with entomopathogenic nematodes in Japan. Technical Bulletin- food and fertilizer technology center 139: 14-22
  13. Hatsukade, M. 1996. Control of turfgrass insect pests with entomopathogenic nematodes in Japan. Proceeding of the international symposium on 'The use of biological control agents under integrated pest management', pp. 78-85
  14. Iwano, H. 1987. Seasonal occurrence of microsporidia in the field populations of the lawn grass cutworm, Spodoptera depravata Butler. Jpn. J. Appl. Ent. Zool. 31: 321-327 https://doi.org/10.1303/jjaez.31.321
  15. Kaya, H.K. 1985. Susceptibility of early larval stages of Pseudaletia unipuncta and Spodoptera exigua (Lepidoptera: Noctuidae) to the entomogenous nematode Steinernema feltiae (Rhabditida: Steinernematidae). J. Invertebr. Pathol. 46: 58-62 https://doi.org/10.1016/0022-2011(85)90129-6
  16. Kaya, H.K., A.M. Koppenh$\"o$fer and M. Johnson. 1998. Natural enemies of entomopathogenic nematodes. japanese J. Nematol. 28: 13-20
  17. Kaya, H.K. and R. Gaugler. 1993. Entomopathogenic nematodes. Ann. Rev. Entomol. 38: 181-206 https://doi.org/10.1146/annurev.en.38.010193.001145
  18. Kim, H.H., H.Y. Choo, C.G. Park, S.M. Lee and Y.M. Choo. 2001a. Biological control of cotton caterpillar, Palpita indica Saunder (Lepidoptera: Pyralidae) with entomopathogenic nematodes. Korean J. Appl. Entomol. 40: 245-252
  19. Kim, H.H., H.Y. Choo, H.S. Lee, C.G. Park, D.W. Lee, B.R. Jin and Y.M. Choo. 2001b. Biological control of LycorielIa mali (Diptera: Sciaridae), a pest of oyster mushroom, Pleurotus ostreatus using entomopathogeiuc nematodes. Korean J. Appl. Entomol. 40: 59-67
  20. Kim, H.H., H.Y. Choo, D.W. Lee, S.M. Lee, H.Y. Jeon, M.R. Cho and M.S. Yiem. 2003. Control efficacy of Korean entomopathogenic nematodes against fungus gnat, Bradysia agrestis (Diptera: Sciaridae) and persistence in bed soil. J. Kor. Soc. Hort. Sci. 44: 393-401
  21. Kondo, E. 1987. Size-related susceptibility of Spodoptera litura (Lepidoptera: Noctuidae) larvae to entomopathogenic nematode, Steinernema feltiae (DD-136). Appl. Ent. Zool. 22: 560-569
  22. Kono, S., M. Taira and H. Tanaka. 1999. Prediction of occurrence of turf insect pests by simulation model, Proceeding of the Kansai Plant Protection Society 41: 39-44
  23. Koppenh$\"o$fer, A.M., S. Ganguly and H.K. Kaya. 2000. Ecological characterization of Steinernema monticolum, a cold-adapted entomopathogenic nematode from Korea. Nematology. 2: 407-416 https://doi.org/10.1163/156854100509268
  24. Kunkel, B.A., D.W. Held and D.A. Potter. 1999. Impect of halofenozide, imidacloprid, and bendiocarb on beneficial invertebrates and predatory activity in turfgrass. J. Econ. Entomol. 92: 922-930
  25. Lee, D.W., H.Y. Choo, H.K. Kaya, S.M. Lee, D.R. Smitly, S.K. Shin and C.G. Park. 2002a. Laboratory and field evaluation of Korean entornopathogenic nematode isolates against the oriental beetle, Exomala orientalis (Coleoptera: Scarabaeidae). J. Econ. Entomol. 95: 918-926 https://doi.org/10.1603/0022-0493-95.5.918
  26. Lee, D.W., H.Y. Choo, O.J. Shin, J.S. Yun and Y.S. Kim. 2002b. Damage of perennial ryegrass, Lolium perenne by chestnut brown chafer, Adoretus tenuimaculatus (Coleoptera: Scarabaeidae) and biological control with Korean isolate of entomopathogenic nematodes. Korean J. Appl. Entomol. 41: 217-223
  27. L$\'o$pez, R. and D.A. Potter. 2000. Ant predation on eggs and larvae of the black cutworm (Lepidoptera: Noctuidae) and Japanese beetle (Coleoptera: Scarabaeidae) in turfgrass. Environ. Entomol. 29: 116-125 https://doi.org/10.1603/0046-225X-29.1.116
  28. Mochida, O. and T. Okada. 1974. A bibliography of Spodoptera spp. (Lepidoptera: Noctuidae). Miscellaneous Bulletin of Kyushu National Agricultural Experiment Station 49: 1-110
  29. Miyasono, M., M. Suzuki, F. Yamashita, C. K.umagai, Y. Ueyama and M. Doteuchi. 1998. Strain of Bacillus thuringiensis serovar kurstaki with increased UV resistence and lepidopteran larvicidal activity. Jpn. J. Appl. Ent. Zoo. 42: 15-20 https://doi.org/10.1303/jjaez.42.15
  30. Oku, T., T. Kobayashi and O. Saito. 1978. Studies on the ecology and control of grassland insects. VIII. notes on the phenology and some behaviours of a grass-worm, Spodoptera depravata Butler, in Morioka. Bulletin of the Tohoku National Agricultural Experimental Station 58: 81-96
  31. Potter, D.A. 1998. Destructive turfgrass insects. 344pp. Ann Arbor Press. Chelsea, USA
  32. Qian, Z.G., G.H. Shen, X.Q. Xia, Q.H. Luo, L. Yang and Y.D. Yuan. 2003. Studies on the biological character and control of Sidemia depravata (Butler). Acta Agriculturae Shanghai. 19: 93-96
  33. Saigusa, M., K. Onozawa, H. Watanabe and K. Shibuya. 1999. Effects of porous hydrate calcium silicate on the wear resistance, insect resistance and disease tolerance of turf grass 'Miyako'. Grassland Science 45: 416-420
  34. Saito, O. 2000. Flight activity of three Spodoptera spp., Spodoptera litura, S. exigua and S. depravata. Physiol. Entomol. 25: 112-119 https://doi.org/10.1046/j.1365-3032.2000.00172.x
  35. Shurtleff, M.C., T.W. Fermanian and R, Randell. 1987. Controlling turfgrass pests. 449pp. Prentice-Hall, INC., Englewood Cliffs, USA
  36. Stock, S.P., H.Y. Choo and H.K. Kaya. 1997a. First record of Steinernema glaseri Steiner, 1929 (Nematoda: Steinernematidae) in Asia, with notes on intraspecific variation. Nematologica 43: 377-38 https://doi.org/10.1163/005025997X00111
  37. Stock, S.P., H.Y. Choo and H.K. Kaya. 1997b. An entomopathogenie nematode, Steinernema monticolum sp. n. (Rhabditida: Steinernematidae) from Korea with a key to other species. Nematologica 43: 15-29 https://doi.org/10.1163/004725997X00025
  38. Stack, S.P., J. Heng, D. Hunt, A.P. Reid, X. Shen and H.Y. Choo. 2001. Redescription of Steinernema longicaudum Shen & Wang (Nematoda: Steinernematidae); geographic distribution and phenotypic variation between allopatric populations. J. Helminthol. 75: 81-92 https://doi.org/10.1079/JOH200036
  39. Terry, L.A., D.A. Potter and P.G. Spicer. 1993. Insecticides affect predatory arthropods and predation on Japanese beetle (Coleoptera: Scarabaeidae) eggs and fall armyworm (Lepidoptera: Noctuidae) Pupae in turfgrass. J. Econ. Entomol. 86: 871-878
  40. Wang, D., K. McSweeney, B. Lowery and J.M. Norman. 1995. Nest structure of the ant Lasius neoniger Emery and its implications to soil modification. Geoderma 66: 259-272 https://doi.org/10.1016/0016-7061(94)00082-L
  41. Woodring, J.L. and H.K. Kaya. 1988. Steinernematidae and heterorhabditid nematodes: a handbook of techniques. Southern Coop. Ser. Bull. 331, Alkansas Agri. Exp. Stn. Fayetteville, AR. 29pp