Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
권호 표지

Variation of Ginkgolides and Bilobalide Contents in Leaves and Cell Cultures of Ginkgo biloba L.

  • Park, Young-Goo (Department of Forestry, Kyungpook National University) ;
  • Kim, Su-Jung (Department of Forestry, Kyungpook National University) ;
  • Jung, Hee-Young (Department of Forest Science, Gyeongsang National University) ;
  • Kang, Young-Min (Department of Forest Science, Gyeongsang National University) ;
  • Kang, Seung-Mi (Department of Forest Science, Gyeongsang National University) ;
  • D. Theertha Prasad (Department of Biotechnology, University of Agricultural Science, GKVK) ;
  • Kim, Sun-Won (Department of Food Nutrition, Gyeongsang National University) ;
  • Park, Myung-Suk (Department of Forest Science, Gyeongsang National University)
  • Published : 2004.01.01
Download PDF
⟨ Previous Next ⟩

Abstract

Ginkgolides (GK) and bilobalide are valuable compounds that belong to the lactone terpene. The contents of these metabolites were determined by HPLC from female and male tree of Ginkgo biloba L. The productivity of G. biloba cells was also compared with the corresponding individual trees. High variations in the ginkgolides and bilobalide were observed from different individuals, plant parts, and cultured cells. The ginkgolides and bilobalide contents were different depending on the plant parts. Callus was obtained from various plant tissues, and NAA was better at callogenesis than 2,4-D in both the female and male trees. The plants and their corresponding cells showed considerable variation in their ginkgolides and bilobalide concentrations. The ginkgolides and bilobalide contents were not correlated with the production between dominant trees and their corresponding cells. Light irradiation enhanced the production of GK-A and GK-B, however, the concentration of bilobalide decreased under dark conditions.

Keywords

References

  1. J. Chromatogr v.600 Superciritical fluid chromatographic analysis of polyphenols in Ginkgo Biloba Huh,H.;E.J.Sraba;J.Singh https://doi.org/10.1016/0021-9673(92)85574-D
  2. Kor. J. Pharmacogn v.21 Flavonoids from the leaves of Ginkgo biloba Kang,S.S.;J.S.Kim;W.J.Kwak;K.H.Kim
  3. Kor. J. Pharmacogn v.21 Identification and quantitative analysis of flavanol glycosides from Ginkgo biloba leaves by high performance liquid chromatography Kang,S.S.;J.S.Kim;W.J.Kwak;K.H.Kim
  4. Phytochemistry v.46 Production of ginkgolide and bilobalide in transformed and gametophyte derived cell cultures of Ginkgo biloba Laurain,D.;J.T.Guiller;J.C.Chenieux;T.A.van,Beek https://doi.org/10.1016/S0031-9422(97)00217-3
  5. Phytochemistry v.48 Distrinbution of ginkgolides and terpenoids niosynthetic activity in Ginkgo biloba Carrier,D.J.;T.A.van,Beek;R. van der Heijden;R.Verporte https://doi.org/10.1016/S0031-9422(97)00450-0
  6. J. Ethnopharmacol v.50 The neuroprotective properties of the Ginkgo biloba leaf: a review of the possible relationship to platelet-activating factor (PAF) Smith,P.F.;K.Maclennan;C.L.Darlington https://doi.org/10.1016/0378-8741(96)01379-7
  7. Bioresource Technol. v.63 Above-ground biomass and nutrient distribution in a 15-year-old ginkgo (Ginkho biloba) plantation in central Korea Son,Y.H;H.W.Kim https://doi.org/10.1016/S0960-8524(97)00070-9
  8. Kor. J. Biotechnol. Bioeng. v.11 Detection of flavonoid compounds by cell culture of Ginkho biloba L Kim,G.S.;Y.W.Paek;K.M.Ko;S.J.Hang;Y.J.Kim;S.J.Chung;B.Hwang
  9. Biotechnology in Agriculture and Forestry;Medicinal and Aromatic Plants v.4 Antitumor compounds; Production by plant cell culture Misawa,M.;T.M.Nakanishio
  10. Physiol. Plant. v.15 A revised medium for rapid growth and bioassays with tobacco tissue culture Murashige,T.;F.Skoog https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  11. Planta Med. v.59 Ontogenic aspects of ginkgolide production in Ginkgo biloba Huh,H.;J.Staba https://doi.org/10.1055/s-2006-959658