Korean Journal of Environmental Biology (환경생물)

Korean Society of Environmental Biology (한국환경생물학회)
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Responses of Cytochrome P450 and EROD Activity in Rockfish (Sebastes schlegeli) Administered Intraperitoneal Injection of 4-nonylphenol

노닐페놀을 주사한 조피볼락의 간장 cytochrome P450과 EROD의 반응

  • 전중균 (강릉대학교 해양생명공학부/동해안해양생물자원연구센터) ;
  • 이지선 (강릉대학교 해양생명공학부/동해안해양생물자원연구센터) ;
  • 손영창 (강릉대학교 해양생명공학부/동해안해양생물자원연구센터) ;
  • 심원준 (한국해양연구원) ;
  • 정지현 (동의대학교 생물학과) ;
  • 홍경표 (한국해양연구원) ;
  • 김병기 (강원도립대학 해양생물자원개발과) ;
  • 한창희 (동의대학교 생물학과)
  • Published : 2004.03.01
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Abstract

Nonyphenol (NP) used actively as non-ionic surfactant is classified as one of most potent endocrine disrupting chemicals. Effects of NP on mixed function oxygenase (MFO) system in rockfish (Sebastes schlegeli) were investigated for seven days after intraperitoneal injection (10 and 25 mg $kg^{-1}$). Hepatosomatic index (BSI) of fishes exposed to NP of 25 mg $kg^{-1}$ was significantly reduced compared to those in control group. NP exposure enhanced cytochrome P450 levels in the fish liver, while 7 ethoxyresorufin-O-deethylase (EROD) activity was inhibited. NP exposure levels in this study were much higher than those found in the coastal environment of Korea. Effects on HSI and liver MFO system, which is involved in steroid hormone metabolism, imply that W may influence on reproduction of fish by not only hormone receptor mediated response but also through effects on the MFO system.

비이온성 계면활성제로 많이 쓰이는 알킬페놀류의 하나인 노닐페놀(nonylphenol)이 해산어류에게 미치는 영향을 조사하려고 주요 양식어종인 조피볼락에게 복강주사로 10 및 25 mg $kg^{-1}$을 1회 투여하였다. 한편 용제인 DMSO만을 주사한 sham구를 설정하여 비교하였다. 주사 후 7일간 간중량지수(hepatosomatic index)의 변화를 조사하였고, 또한 간장 미크로좀 중 대표적인 약물대사효소인 cytochrome P450 (CYP) 농도와 ethoxyresorufin deethylase (EROD) 활성의 변화도 측정하였다. 그 결과, 고농도(25 mg $kg^{-1}$) 주사구에서는 HSl가 감소하였는데, 이것은 NP가 간장에 독성이 있다는 것을 보여준다. 한편, 약물대사효소 중 CYP 농도는 유도되었지만 EROD 활성은 저해되는 경향을 보였다. 이번에 주사한 농도는 우리나라 연근해 해수 중의 NP 농도에 비해 매우 높은 수준이지만, 본 실험에서는 NP가 조피볼락에 게간장 독성을 보였고, 간장의 약물대사효소에도 영향을 미친다는 것을 확인할 수 있었다.

Keywords

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