Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Effects of Monovalent Cations on the βReaction Kinetics of Tryptophan Synthase

트립토판 합성효소의 β반응속도에 미치는 일가양이온의 영향

  • Kim, Il (Department of Molecular Biology, College of Natural Sciences, Pusan National University) ;
  • Shin, Hye-Ja (Environmental Engineering Major, Division of Applied Engineering, Dongseo University) ;
  • Im, Woon-Ki (Department of Molecular Biology, College of Natural Sciences, Pusan National University) ;
  • Kim, Han-Do (Department of Molecular Biology, College of Natural Sciences, Pusan National University)
  • 김일 (부산대학교 자연과학대학 분자생물학과) ;
  • 신혜자 (동서대학교 응용공학부 환경공학전공) ;
  • 임운기 (부산대학교 자연과학대학 분자생물학과) ;
  • 김한도 (부산대학교 자연과학대학 분자생물학과)
  • Published : 2004.02.01
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Abstract

Effects of monovalent cations were examined on the fast $\beta$reaction of $\alpha$D56N and $\alpha$D56G mutant tryptophan synthase. Reaction rates for the production and degradation of intermediates in the reaction were changed in the presence of cathons. The mutant proteins showed different reaction rates from those of wild-type protein, and additional changes occurred in the presence of cations. The results showed that monovalent cations and $\alpha$D56 are important in allosteric properties of this protein.

대장균 트립토판 합성효소의 $\beta$반응의 빠른 속도 반응에 일가 양이온의 영향을 D56N과 D56G 잔기치환체를 대상으로 조사하였다. 일가 양이온을 처리하였을 때 생성되는 중간반응 생성물의 생성과 분해 속도가 영향을 받았다. 56번 자리 잔기치환체도 야생형에 비해 반응의 중간산물의 생성과 분해에 있어 모두 느린 속도를 보여주고 있다. 이들 잔기 치환체에 미치는 일가 양이온의 영향 또한 달라졌다. 이 결과는 대장균의 효소에서도 56번잔기가 이소조절에 관여하고 있고, 이 과정에서 일가 양이온들이 이소조절 리간드역할을 수행하는 것을 보여준다.

Keywords

References

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