Journal of Aquaculture (한국양식학회지)

The Korean Society of Fisheries and Aquatic Science (한국양식학회)
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Effects of Cryoprotectants and Freezing Rates on Cryopreservation of Catfish, Silurus asotus Sperm

  • Kho, Kang Hee (Department of Aquaculture, Yosu National University) ;
  • Kang, Kyoung Ho (Department of Aquaculture, Yosu National University)
  • Published : 2004.02.01
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Abstract

Milt of the catfish was stripped into immobilizing solution containing 175 mM NaCl and 30 mM Tris at pH 7.8 and was successfully cryopreserved after a stepwise freezing procedure. After stepwise thawing, motility of spermatozoa was slightly lower than that of fresh sperm. Batches of 40-80 eggs were fertilized with cryopreserved spermatozoa, after thawing and activation in solution containing 50 mM NaCl, 20 mM Tris and HCl at pH 7.8; this resulted in 62.2% fertilization success, compared to 70.6 % with fresh sperm.

Keywords

References

  1. Natyre v.172 Sperm storage and cross-fertilization of spring and autumn spawning herring Blaxter,J.H.S.
  2. Aquaculture v.5 Study on crogenic preserv ation of grey mullet sperm Chao,N.H.;H.P.Chen;J.C.Liao
  3. J. Appl. Ichthyol. v.5 La cryconservation de la laitance de la carpe, Cyprinus carpio Cognie,F.;R.Billard;N.H.Chao https://doi.org/10.1111/j.1439-0426.1989.tb00489.x
  4. Cell Motil. Cytoskel. v.14 Rise of internal &Ca^{2+}& accompanies the initiation of trout sperm motility Cosson,M.P.;P.;J.Cosson;L.Letellier https://doi.org/10.1002/cm.970140312
  5. Cryo-letters v.1 Cryopreservation of the brown, brook and rainbow trout sperm Erdahl,D.A.;E.F.Graham
  6. Cryobiology v.40 Cryopreservation of seabream (Sparus aurata) spermatozoa Fabbrocini,A.;S.Lubrano Lavadera;S.Rispoli;G.Sansone https://doi.org/10.1006/cryo.1999.2220
  7. Aquaculture v.28 A comparative study of various extenders for milkfish, Chanos chanos (Forsskal), sperm preservation Hara,S.;J.T.Canto;J.M.E.Almendras https://doi.org/10.1016/0044-8486(82)90076-X
  8. Aquaculture v.32 Cryopreservation of Sarotherodon mossambicus spermatozoa Harvey,B. https://doi.org/10.1016/0044-8486(83)90228-4
  9. In: 4th international Sympossium on Reproductive Physiology of Fish A simple saccharide extender for cryopreservation of rainbow trout, Oncorhynchus mykiss sperm Holtz,W.;R.Schmidt-Baulatin;M.Meiners-Gefken
  10. J. Shimonoseki Coll. Fish. v.11 Foundation studies in culturing of the Japanese loach, Misgurnus anguillicaudatus: Storing of spermatozoon Kubota,Z.
  11. Aquaculture v.115 Cryopreservation of European catfish, Silurus glanis L. spermatozoa Linhart,O.;R.Billard;J.P.Proteau https://doi.org/10.1016/0044-8486(93)90148-R
  12. Cryobiology v.168 The freezing of biological systems Mazur,P.
  13. Selection, Hybridization and Genetic Engineering in Aquaculture v.1 Cryopreservation of European catfish, Silurus glanis, sperm Marian,T.;Z.L.Krasznai;K,Tiews,(ed.)
  14. J. Fish. Res. Board Can. v.25 Cryogenic preservation of Atlantic cod, Gadus mothua, sperm Mounib.M.S.;P.C.Hwang;D.R.Idler https://doi.org/10.1139/f68-232
  15. J. Aqua. Trop. v.10 Cryopreservation of spermatozoa of two Asian freshwater catfish, Heteropneutes fossilis and Clarias batrachus Padhi,B.K.;R.K.Mandal
  16. Asia. Fish. Sci. v.7 Chilled storage of pikey bream, Acanthopagrus berda, sperm and activation in different salinities Palmer,P.J.
  17. J. Fish Biol. v.22 Cryopreservation of spermatozoa of the whitefish, Coregonus muksun Pallas Piironen,J.;H.Hyvarinen https://doi.org/10.1111/j.1095-8649.1983.tb04736.x
  18. Methods in Molecular Biology Cryopreservation of fish spermatozoa Rana,K.J.;J.G.day(ed.);M.R.McLellan(ed.)
  19. J. Fish Biol. v.17 A review of the biology, handling and storage of salmonid spermatozoa Scott,A.P.;S.M.Baybes https://doi.org/10.1111/j.1095-8649.1980.tb02804.x
  20. Aquaculture v.63 The fertilizing capacity of cryopreserved sharptooth catfish (Clarias gariepinus) sperm Steyn,G.J.;J.H.Van Vuren https://doi.org/10.1016/0044-8486(87)90070-6
  21. Fish. Sci. v.60 Motility of pejerrey, Odontesthes bonariensis spermatozoa Strussmann,C.A.;P.Renard;H.Ling;F.Takasima https://doi.org/10.2331/fishsci.60.9
  22. Biostatistical Analysis Zar,J.H.