Journal of the East Asian Society of Dietary Life (동아시아식생활학회지)

The East Asian Society of Dietary Life (동아시아식생활학회)
권호 표지

Acetycholinesterase Inhibiting Effect and Free Radical Scavenging Effect of Soybean(Glycine max) and Yak-Kong(Rhynchosia nolubilis)

대두와 약콩의 Acetylcholinesterase 활성 억제효과와 항산화 효과

  • 강순아 (건국대학교 응용생물화학과 생명분자정보학센터) ;
  • 한진아 (서울대학병원 임상영양과)
  • Published : 2004.02.01
Download PDF
⟨ Previous Next ⟩

Abstract

The deficiency of the neurotransmitter acetylcholine(ACh) is responsible for the initiation of Alzheimer's disease. In addition, there is a simple evidence that oxidative stress significantly increases in persons with Parkinson's disease compared to age-matched healthy persons. Therefore, the objective of the study was to investigate the neurodegeneration inhibitory effect of soybean(Glycine Max) and Yak-Kong(Rhynchosia Nolubilis) by measuring the degree of inhibiting Acetylcholinesterase (AChE) catabolizing the ACh and the free radical scavenger effect in vitro. AChE was measured by the minor modified Ellman assay. Free radical scavenging activity was measured using l-diphenyl-2-picrylhydrazyl (DPPH). First, the MeOH extracts of Soybean and Yak-Kong showed the AChE inhibiting activity of 62.0$\pm$2.43％ and 65.0$\pm$3.29％ at the 5 mg/$m\ell$ concentration. The 50％ inhibitory concentration ($IC_{50}$/) of AChE was 1.96 and 1.31 mg/$m\ell$ in the MeOH extracts of soybean and Yak-Kong. Second, the MeOH extracts of soybean and Yak-Kong showed the free radical scavenger activity of 23.1$\pm$4.26％ and 80.7$\pm$4.61％ at the 5 mg/$m\ell$. IC50 of free radical scavenger activity in Soybean and Yak-Kong was 13.00 and 1.41 mg/$m\ell$ in MeOH extracts and was 5.95 and 2.74 mg/$m\ell$ in hot-water extracts, respectively. In this study, the extracts of Soybean and Yak-Kong showed powerful effects in the AChE inhibition and free radical scavenging. The extracts of Soybean and Yak-Kong were expected to prevent the many neurodegenerative diseases.

본 연구에서는 이미 항산화 효과로 알려져 있는 대두와 약성이 탁월한 약콩에서 AChE 활성 억제효과와 Free radical scavenger 효과를 노화로 인한 질병 예방효과에 대한 기초자료를 제공하고자 수행하였다. 1. 대두 열탕 추출물의 경우 저농도인 1, 0.1 mg/$m\ell$에서는AChE의 활성 억제 효과가 메탄을 추출물보다 높았다. (P<0.001) 2. 대두, 약콩 메탄올 추출물에서는 고농도인 5 mg/$m\ell$에서 각 시료의 효과가 62.0$\pm$2.43%, 65.0$\pm$3.29%로 비슷했으나 저농도인 0.1 mg/$m\ell$에서는 약콩의 경우 25.5$\pm$0.94%로 약콩이 대두에 비해 유의적인 AChE 억제효과를 나타냈다. (p<0.001) 3. 시간에 따른 Free radical scavenger효과는 비타민 C의 경우 시간에 따라 비슷한 효과를 보였으나 대두와 약콩은 incubating 시간에 따라 free radical 억제효과가 증가해 높은 항산화 효과를 볼 수 있었다. 4. 대두, 약콩 메탄올 추출물에서는 약콩이 고농도인 5mg/$m\ell$에서 약4배 정도의 효과를 보였으며 Free radical scavenger의 50%효과를 나타내는 $IC_{50}$/에서는 약 10배 정도로 높은 Free radical scavenger 효과를 나타내 약콩이 대두에 비해 유의적으로 높은 효과를 나타내었다. 결론적으로, 대두와 약콩의 추출물은 AChE 활성억제효과에 있어서 좋은 효과를 나타내며 또한 노화의 원인과 질병의 원인이 되는 free radical을 억제하는 효과를 가지고 있어 노인성 치매를 비롯하여 파킨슨씨병, 뇌허혈 및 뇌졸중과 같은 각종 노인성 질환예방에 도움이 될 것으로 보인다.

Keywords

References

  1. 世界有用植物事典 堀田 萬ら(編輯)
  2. Public Health Nutr v.2 Health potential of soy isoflavones for menopausal women Anderson,J.J.;Anthony,M.S.;Cline,J.M.;Washbum,S.A.;Garmer,S.C.
  3. J Nutr v.126 Soybean isoflavones improve cardiovascular risk factors without affecting the reproductive system of peripubertal rhesus monkeys Anthony,M.S.;Clarkson,T.B.;Hughes,C.L.Jr.;Morgan,T.M.;Burke,G.L.
  4. Phytother Res v.17 Effects of soybean ethanol extract on the cell survival and oxidative strss in osteoblastic cells Choi,E.M.;Koo,S.J.
  5. Daily Economics v.9
  6. J Environ Sci Health v.38 Protective effects od isoflavone on some biochemical parameters affected by cypermethrin in mal rabbits el-Demerdash,F.M.;Yousef,M.I.;Al-Salhen,K.S.
  7. Biochem Pharmacol v.7 A new and rapid colorimetic determination of acetylcholinesterase activity Ellman,G.L.;Courtney,K.D.;Andres,V.;Featherstone,R.M.
  8. The Gerontologist v.35 Relationships among age, exercise, health, and cognitive function in a British sample Emery,C.F.;Huppert,F.A.;Schein,R.L.
  9. Psycho-phamacology v.157 Eating soya improves human memory File,S.E.;Jarrett,N.;Fluck,E.;Duffy,R.;Casey,K.;Wiseman,H.
  10. Neurology v.41 Prevalence of Alzheimer's disease and other dementias in sex and education Fratiglioni,L.;Grut,M.;Forsell,Y.
  11. J Neurochem v.59 Reactive oxygen species and the central nervous system Halliwell,B.
  12. J Korean Soc Food Nutr v.23 The effects of high fat diet and dietary fiber on lipid metabolism in rats Han,J.S.;Han,Y.B.
  13. Am J Epidemiol v.144 Dietary antioxidants and cognitive function in a population-based sample of older persons-the Rotterdam Study Jama,J.W.;Launer,L.J.;Witteman,J.C.M.;Breeijen,J.H.;Breteler,M.M.B.;Grobbee,D.E.
  14. Nutr Neurosci v.5 Safety of soy-derived phosphatidylserine in elderly people Jorissen,B.L.;Brouns,F.;Van Boxtel,M.P.;Riedel,W.J.
  15. J of ARAHE v.8 High performance liquid chromatographic analysis of isoflavones in soybean and blackbean Kang,S.A.;Jang,K.H.;Cho,Y.H.;Hong,K.H.;Kong,S.H.;Choue,R.W.
  16. Korean J Food Sci Technol v.32 High performance liquid chromatographic analysis of isoflavones in soybean foods Kim,C.;Lee,Y.S.;Kim,J.S.;Hahn,Y.
  17. RDA J Crop Sci v.40 A black seed coat soybean variety with small seed and lodging resistant Tawonkong Kim,S.D.;Park,K.Y.;Lee,Y.H.;Yun,H.T.;Lee,S.H.;Kim,Y.H.;Seung,Y.K.;Park,E.H.;Kim,H.S.;Ryu,Y.H.;Son,Y.G.;Kim,Y.S.
  18. Korea J Crop Sci v.41 Physiological function of isoflavones and their genetic and environmental variation in soybean Kim,Y.H.;Kim,S.K.
  19. Annual Report Korea Health and Welfare Office
  20. J Neurol v.249 Free radicals in Parkinson's disease Koutsiliery,E.;Scheller,C.;Grunblatt,E.;Nara,K.;Li,J.;Riederer,P.
  21. Menopause v.10 Isoflavones and cognitive function in older women; the SOy and Postmenopausal Health In Aging (SOPHIA) study Kritz-Silverstein,D.;Von Muhlen,D.;Barrett-Connor,E.;Bressel,M.A.
  22. Health for Future Foods Health function of soybean foods in proceeding of IUFOST '96 reginal symposium on non-nutrition Kwon,T.W.;Song,Y.S.
  23. Ph. D. Dissertation. Kyung Hee University Effect of black bean and sam-ryung-bak-chul-san on ovariectomy-induced postmenopausal osteoporotic rats Lee,H.K.
  24. J Nutr Sci Vitaminol v.247 Effect of supplementation of vitamin E and vitamin C on brain acetylcholinesterase activity and neurotransmitter levels in rats treated with scopolamine, an inducer of dementia Lee,L;Kang,S.A.;Lee,H.O.;Lee,B.H.;Jung,I.K.
  25. Public Health v.115 Relationships between dietary intake and cognitive function level in Korean elderly people Lee,L.;Kang,S.A.;Lee,H.O.;Lee,B.H.(et al.)
  26. Ying Yong Sheng Tai Xue Bao v.14 Effect of short-term phenanthene stress on SOD activities and MDA contents in soybean (Glycine max) seedlings Liu,W.;Li,P.;Zhou,Q.;Sun,T.;Tai,P.;Xu,H.;Zhang,H.
  27. J of ARAHE v.6 Associations between Environmental Factors and Depression in Korean Elderly People Park,J.S.;Kang,S.A.;Lee,L.;Kim,J.H.;Jung,I.K.;Park,Y.J.;Lee,J.E.
  28. J Nutr v.125 Modern application for ancient bean soybeans and the prevention and treatment of chronic disease Messina,M.
  29. J Biochem Taxicol v.11 Maturational differences inchlorphyrifos-oxonase activity may contribute to age-related sensitivity to chlorpyrifos Mortensen,S.R.;Chanda,S.M.;Hooper,M.J.;Padilla,S.
  30. Losing a million minds (OTABA-323) Office of Technology Assessment (OTA)
  31. J Pharmacol Exp Ther v.274 Cholinesterase inhibitors propsosed for treating dementia in Alzheimer's disease: selectivity toward human brain acetylcholinesterase compared with butyrylcholinesterase Pacheco,G.;Palacios-Esquivel,R.;Moss,D.E.
  32. J Neurosci v.10 Excitatory amino acid release and free radical formation may cooperate in the genesis of ischemia-induced neuronal damage Pellegrini-Giampietro,D.E.;Cherici,G.;Alesiani,M.;Carla,V.;Moroni,F.
  33. J Nutr v.133 Total antioxidant capacity of plant foods, beverages and oils consumed in Italy assessed by three different in vitro assays Pellegrini,N.;Serafini,M.;Colombi,B.;Del Rio D;Salvatore,S.;Bianchi,M.;Brighenti,F.
  34. Am J Clin Nutr v.55 Nutritional factors in physical and cognitive functions of elderly people Rosenberg,I.H.;Miller,J.W.
  35. Am J Clin Nutr v.65 Nutritional status and cognitive functioning in a normally aging sample: a 6-y reassessment La Rue A.;Koehler,K.M.;Wayne,S.J.;Chiulli,S.J.;Haaland,K.Y.;Garry,P.J.
  36. Postgrand Med v.105 What's new in Alzheimer's disease treatment? Reasons for optimism about future pharmacologic options Shadlen,M.F.;Larson,E.B.
  37. JAMA v.271 Influence of education and occupation on the incidence of Alzheimer's disease Stern,Y.;Gurland,B.;Tatemichi,T.K.;Tang,M.X.;Wilder,D.;Mayeux,R.
  38. Neurology v.35 Prevalence of severe dementia in Finland Sulkava,R.;Wikstrom,J.;Aromaa(et al.)
  39. Nutrition and Cancer v.20 Inhibition of tumor promotor-induced hydrogen peroxide formation in vitro and in vivo by genistein Wei,H.;Wei,L.;Frenkel,K.;Bowen,R.;Bames,S.
  40. J Am Coll Nutr v.19 Brain agind and midlife tofu consumption White,L.R.;Petrovitch,H.;Ross,G.W.;Masaki,K.;Hardman,J.;Nelson,J.;Davis,D.;Markesbery,W.