Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Morphological Characteristics and Genetic Relationship by RAPD Marker in Iris spp.

자생붓꽃의 형태적 특성 및 RAPD 마커에 의한 유연관계 분석

  • 홍성미 (대구가톨릭대학교 생명자연학부) ;
  • 고재철 (대구가톨릭대학교 생명자연학부)
  • Published : 2004.03.01
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Abstract

This study was conducted to observe the morphological characteristics and to analyse genetic relationship using RAPD markers in 9 species of Iris. The period of flowering was shown very broad among 9 species of Iris, from April 22 to June 9. The flower stalk was fluctuated from 9.0cm (I. rossii) to 104.0cm (I. ensata). I. ensata has the largest flower size and followed by I. laevigata, I. tectorum, I. sanguinea, I. pseudacorus, I. lactea, I. rossii, I. minutoaurea, I. odaesanensis. Among the 108 amplified bands by PCR with 8 random primers, 107 showed polymorphism and only one showed monomorphism among 9 species of Iris. The average dissimilarity coefficient among 9 species of Iris was 0.252. The range of dissimilarity coefficient was shown as from 0.095 to 0.609. Nine species were divided into three groups. The first group was I. tectorum, and the second group was I. lactea, I. laevigata, I. pseudacorus, I. odaesanensis. I. minutoaurea, I. rossii, I. sanguinea, I. ensata were belong to group three.

본 연구는 붓꽃속 식물의 형태적 특성 조사와 RAPD분석을 통해 유전적 다양성과 근연성을 밝히고 유전자 도입을 위한 기초적인 자료를 얻기 위하여 실시하였다. RAPD를 이용하여 국내에서 수집한 자생붓꽃종의 DNA다형성을 조사한 결과 40개의 primer중 8개의 유용한primer가 선발되었고 이들 primer는 108개의 밴드를 나타내었다. 이들 중 다형성 밴드는 107 개였다. Jaccard (1-S$_{J}$ )법에 의한 비유사도 계수는 0.095∼0.699로 나타났고, dendrogram에서 나타난 유전적 근연성은 크게 세 개의 군으로 분류되었다. I군은 연미붓꽃, II군은 타래붓꽃, 제비붓꽃, 노랑꽃창포, 노랑무늬붓꽃이 속해 있었으며, III군은 금붓꽃, 각시붓꽃, 붓꽃, 꽃창포가 속해 있었다.

Keywords

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