한국식품저장유통학회지 (Food Science and Preservation)

  • 제11권1호
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  • Pages.71-78
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  • 2004
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  • 3022-5477(pISSN)
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  • 3022-5485(eISSN)
한국식품저장유통학회 (The Korean Society of Food Preservation)
권호 표지

매실 추출물이 알코올 투여 흰쥐의 항산화계 및 지질과산화에 미치는 영향

Effects of Ethanol Extract of Prunus mume on the Antioxidative System and Lipid Peroxidation on Ethanol-Induced Hepatotoxicity in Rat Liver

  • 이정현 (조선대학교 식품영양학과) ;
  • 나명순 (조선대학교 식품영양학과) ;
  • 이명렬 (조선대학교 식품영양학과)
  • 발행 : 2004.03.01
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초록

매실 에탄올추출물이 알코올투여로 유발된 횐쥐의 간손상에 미치는 영향을 알아보기 위하여 실험동물을 정상군, 매실 에탄올추출물 200 mg/kg 투여군, 알코올투여군(35% ethanol 10 mL/kg, b.w./day), 알코올 및 매실 에탄올추출물 200 mg/kg 병합투여군과 알코올 및 매실 에탄올추출물 400 mg/kg 병합투여군의 5군으로 나누어 6주간 사육하였고, 체중증가율 및 식이효율, 혈청중 ALT와 AST활성, 간손상 억제 효과를 확인하기 위해 SOD, catalase, xanthi ne oxidase 및 GSH-Px활성을 측정하고, 지질과산화물인 thiobarbituric acid reactant(TBARS) 및 GSM함량에 미치는 영향을 측정하였다. 그 결과, 매실 에탄올추출물이 알코올투여로 증가된 유리기해독계 효소인 GSH-Px활성을 유의성 있게 감소시키고 비효소적 항산화작용을 나타내는 GSH함량을 증가시킴으로서 지질과산화물에 대한 방어력이 증강됨을 보였고, 알코올 투여로 상승된 ALT 및 AST활성을 유의성있게 감소시켜 매실 에탄올추출물이 에탄올에 의한 지방간 또는 손상된 간세포를 회복시키는 작용이 있는 것으로 추정되었다.

To investigate the antioxidative effects of Prunus mume ethanol extract on the ethanol-induced hepatotoxicity in rat liver, Sprague-Dawley rats weighing 120∼160 g were divided into 5 groups; normal group(NOR), Prunus mume ethanol extract 200mg/kg treated group(PME), ethanol(10 mL/kg, 35％) treated group(ETH), Prunus mume ethanol extract 200 mg/kg and ethanol treated group (PML) and Prunus mume ethanol extract 400 mg/kg, and ethanol treated group(PMF), respectively. The antioxidative activity in vitro was reduced in order of EtOAC＞n-hexane＞water＞ chloroform fraction. The growth rate and feed efficiency ratio decreased by ethanol administration were gradually increased to the adjacent level of NOR by administering Prunus mume ethanol extract. It was observed that activities of catalase, superoxide dismutase(SOD), xanthine oxidase and glutathione peroxidase(GSH-Px) of liver and alanine aminotransferase(ALT) and asparate aminotransferase(AST) of serum were elevated by ethanol administration. Besides, Prunus mume ethanol extract markedly decreased elevated activites of catalase, GSH-Px, ALT and AST, except in activites of SOD and xanthine oxidase compared to ETH. Also, the depleted content of GSH by ethanol was increased similar to NOR level by administering Prunus mume ethanol extract. These results suggested that Prunus mume ethanol extract has a possible protective effect on the ethanol-induced hepatotoxicity in rat liver.

키워드

참고문헌

  1. 이창복 (1979) 대한식물도감, 향문사, 서울, p.451
  2. 문관심 (1994) 약초의 성분과 이용, 일월서각, 서울, p.299
  3. Han, J.T., Lee, S.Y., Kim, K.N. and Baek, N.I. (2001) Rutin, antioxidant compound isolated from the fruit of Prunus mume. J. Korean Soc. Agric. Chem. Biotechnol., 44, 35-37
  4. 佐藤公一.森英男 外三人 (1972) 日本果樹園藝大事典. 養賢堂, 東京, P.720-737
  5. 赤松金芳 (1997) 新訂和漢藥. 東京: 醫齒藥出版(株). p.396
  6. Lee, D.S., Woo, S.K. and Yang, C.B. (1972) Studies on the chemical composition of major fruits in Korea. Korean J. Food Sci. Technol., 4, 134-139
  7. Moon, J.S. (1994) Changes in physiochemical properties of korean plum(Prunus mume)during ripening. M.S. Thesis. Kyunghee Univ. Korea
  8. Song, B.H., Choi, K.S. and Kim, Y.D. (1997) Changes of physiocochemical and flavor components of Ume according to varieties and picking date. Korean J. Post-Harvest Sci. Technol. Agri. Products., 4, 77-85
  9. 권영주 . 김영희 . 곽재진 . 김근수 . 양광규 (1990) 살구화 매실의 휘발성 향기성분. 한국농화학회지, 33, 319-324
  10. 강민영 . 정윤화 . 은종방 (1999) 매실과유과 매실착즙박의 이화학적 특성. 한국식품과학회지, 31, 1434-1439
  11. 차환수 . 황진봉 . 박정선 . 박용곤 . 조재선 (1999) 매실의 성숙중 유기산, 유리당 및 유리아미노산의 변화. 농산물저장유통학회지, 6, 481-487
  12. 배지현 . 김기진 . 김성미 . 이원재 . 이선장 (2000) 매실추출물을 함유한 기능성 음료개발. 한국식품과학회지, 32, 713-719
  13. Youn, M.S. (1989) Effects of Maesil extracts ingestion on blood lactate density and serum lipid cpmponents. M.S. Thesis. Kyungnarn Univ. Korea
  14. Sheo, H.J, Lee, M.Y. and Chung, D.L. (1990) Effects of Prunus mume extracts on the gastric secretion in rats and carbon tetrachloride induced liver damage of rabbits. Korean J. Food Sci. Nutr., 19, 21-26
  15. Sheo, H.J., Ko, E.Y. and Lee, M.Y. (1987) Effects of Prunus mume extracts on experimentally alloxan induced diabetes in rabbit. Korean J. Food Sci. Nutr., 16, 41-47
  16. Bae, J.H. and Kim, G.J. (1999) Effects of Prunus mume extract containing beverage on the proliferation of food-borne pathogens. J. East Asian Diet. Life., 9, 214-222
  17. Shim, K.H., Sung, N.K., Choi, J.S. and Kang, K.S. (1989) Changes in major components of Japenese apricot during ripening. J. Korean Soc. Food Nutr., 18, 101-108
  18. Shim, J.H., Park, M.W., Kim, M.R., Lim, K.T. and Park, S.T. (2002) Screening of antioxidant in frutus mume(Prunus mume Sieb. et Zucc.) extract. J. Korean Soc. Agric. Chem. Biotechnol., 45, 119-123
  19. 우원식(1997) 천연물 화학 연구. 서울, 서울대학교 출판부. p.14-15
  20. Fujji, M., Ohmachi, T., Sagami, I., and Watanabe, M. (1985) Liver microsomal drug metabolism in ethanol treated hamsters. Biochem. Pharmacol., 34, 3881-3885 https://doi.org/10.1016/0006-2952(85)90438-1
  21. Downey,, J.M., Miura, Y., Eddy, L.J., Chambers, D.E., Mellert, T., Hearse, D.J. and Yellon, D.M. (1987) Xanthine oxidase is not a source of free radicals in the ischemic rabbit heart. J. Mol. Cell Cardiol., 19, 1053-1060 https://doi.org/10.1016/S0022-2828(87)80350-4
  22. Crapo, C.H., McCord, J.M. and Fridovich, I. (1978) Preparation and assay of superoxide dismutase. Methods enzymol. ed. Fleischer S and Packer L., Academic press, New York, p.382-393
  23. Aebi, H. (1974) Catalase, methods of enzymatic analysis. Bergmeyer HU, Bergmeyer, J and Grabi, M., eds. 3rd ed., Verlag. chemie., p.673-689
  24. Flohe, L., Wolfgng, A. and Gunzler, W.A. (1984) Assay of glutathine peroxidase. In Methods in enzymatic analysis. Packer. L. eds. Academic Press, Inc., New York, p.673-684
  25. Buege, J.A. and Aust, S.D. (1969) Microsomal lipid peroxidation. In Methods in enzymoogy. Packer, L. ed., Academic Press, New York, p.502-520
  26. Tietze, F. (1969) Enzymatic methods for quantitative detennination of nanogram amounts of total and oxidized glutathione. Anal. Biochem., 27, 502-522 https://doi.org/10.1016/0003-2697(69)90064-5
  27. Reitman, S. and Frankel, S. (1957) A colorimetric method for the determination of serum glutamic oxaloacetic detennination and glutamic pyruvic transaminase. Am. J. Clin. Pathol., 28, 56-63 https://doi.org/10.1093/ajcp/28.1.56
  28. Lowry, C.H., Rsenbrough, N.J., Farr, A.L., and Randall, R.J. (1951) Protein measurement with folin phenol reagent. J. Biol. Chem., 193, 265-275
  29. Shaw, S. and Lieber, C.S, (1983) Nutrition and alcohol, A clicnical perpective, Weininger J. Briggs GM, eds. Nutrition Update John Wiley & Sons, New York, p.79-104
  30. Halsted, C.H. (1980) Alcoholism and malnutrition introduction to the sympaium. Am. J. Clin. Nutr., 33, 2705-2708 https://doi.org/10.1093/ajcn/33.12.2705
  31. Mitchell, M.C and Herlong, H.F. (1986) Alcohol and nutritional: caloric value, bioenergigetics, and relationship to liver damage. Ann. Rev. Nutr., 6, 457-164 https://doi.org/10.1146/annurev.nu.06.070186.002325
  32. Koo, B.K., Chung, J.M, Lee, H.S. (1998) Biochemical evaluation of nutritional status of protein and lipid in patients with alcoholic liver disease. Korean J. Food Sci. Nutr., 27, 1236-1243
  33. Zimmerman, H.J. (1981) Chemical hepatic injury and its detection, in toxicology of the liver. Plaa, G.L. and Hewitt, W.R., eds., Raven press, p.1-10
  34. Goldberg, B. and Stem, A (1977) The role of the superoxide anion as a toxic species in the erythrocyte. Arch. Biochem. Biophy., 178, 218-225 https://doi.org/10.1016/0003-9861(77)90187-4
  35. 윤종국, 전태원, 오만진, 이규희 (2000) 흰주에 있어서 구기자 알콜 추출물이 Oxygen free radical 및 alcohol 대사 요소 활성에 미치는 영향. 한국식품영양과학회지, 29, 268-273
  36. Chow, C.K. and Tappel. A.L. (1974) Response of glutathione peroxidase to dietary selenium in rats. J. Nutr., 104, 444-451 https://doi.org/10.1093/jn/104.4.444
  37. Leibovitz. B.E. and Siegel. B.V. (1980) Aspects of free radical reaction in biological system. Aging. J. Gerontol., 35, 45-56 https://doi.org/10.1093/geronj/35.1.45
  38. Park, J.A, Kim, M.K. (1999) Effect of korean native plant diet on lipid metabolism, antioxidative capacity and cadmium detoxification in rats. The Korean Nutr. Soc., 32, 353-368
  39. Harris E.D. (1992) Regulation of antioxidant enzymes. J. Nutr., 122, 625-626 https://doi.org/10.1093/jn/122.suppl_3.625
  40. Yagi, K. (1987) Lipid peroxides and human disease. Chem. Phys. Lipid, 45, 337-354 https://doi.org/10.1016/0009-3084(87)90071-5
  41. Storch, J. and Ferber, E. (1988) Detergent-amplified chemiluminescence of lueigenin for detennination of superoxide anion production by NADPH oxidase and xanthine oxidase. Anal. Biochem., 169, 262-275 https://doi.org/10.1016/0003-2697(88)90283-7
  42. Oei. H.H., Stroo. W.E., Burton. K.P. and Schaffer. S.W. (1982) A possible role of xanthine oxidase in producing oxidative stress in the heart of chronically ethanol treated rats. Res. Commun Chem. Pathol. Pharmacol., 38, 454-461
  43. Chance, B., Sies, H. and Boveris, A. (1979) Hydroperoxide metabolism in mammalian organs. Physiol. Rev., 59, 527-605 https://doi.org/10.1152/physrev.1979.59.3.527
  44. Frank, L. and Massaro, D. (1980) Oxygen toxicity. Am. J. Med., 69, 117-126 https://doi.org/10.1016/0002-9343(80)90509-4
  45. Dolphin, D., Forman, A., Berg, D.C., Fajce, J. and Felton, R.H. (1971) Compounds of catalase and horeradish peroxidase. Proc. Natl. Acad. Sci. USA, 68, 614-623 https://doi.org/10.1073/pnas.68.3.614
  46. Tubaro, E., Banci, F., Looti. B. and Gorce, C. (1976) Xanthine oxidase activation in animal liver during infectious processes. Arzneimittel-Forschung., 26, 2185-2198
  47. McCord, J.M. and Fridovich, I. (1969) Superoxide dismutase an enzymatic function for erythrocuprein(hemocuprin). J. Biol. Chem., 244, 6049-6061
  48. Fred, J., Yost, J. and Fridovich, I. (1976) Superoxide and hydrogen peroxide in oxygen damage. Arch. Biochem. Biophys., 175, 514-525 https://doi.org/10.1016/0003-9861(76)90539-7
  49. Recknagel, R.O., Glende, E.A. and Hruszkewyez, A.M. (1977) Chemical mechanisms in carbon tetrachloride toxicity in 'Free radicals in biology' Pryor, W. A. ed., Academic Press, New York, p.295-314
  50. Plaa, G.L. and Witschi, H. (1976) Chemical drugs and lipid peroxidation. Annu. Rev. Pharmacol Toxicol., 16, 125-141 https://doi.org/10.1146/annurev.pa.16.040176.001013
  51. Suematsu, T., Matsumura, T., Sato, N., Miyamoto, T., Ooka, T. and Abee. H. (1981) Lipid peroxidation in alcoholic liver disease in humans. Alcohol, Clin. Exp. Res., 5, 427-439 https://doi.org/10.1111/j.1530-0277.1981.tb04926.x
  52. Schilling, R.Z. and Reitz, R.C. (1980) A mechanism for ethanol-induced damage to liver mitochondrial structure and function. Biochimica Biophysica Acta., 603, 266-285 https://doi.org/10.1016/0005-2736(80)90373-9
  53. Saunders JB, Williams R. (1983) The genetics of alcoholism. Alcohol. 18, 189-211 https://doi.org/10.1016/S0741-8329(99)00003-8
  54. Sevanian, A. and Hochstein, P. (1985) Mechanism and consequences of lipid peroxidation in biological system. Ann. Rev. Nutr., 365-373
  55. Reed, D.J. and Fariss, M.W. (1984) Glutathione depletion and susceotibility. Pharmacol Rev., 36, 25-28
  56. Lieber, C.S. (1980) Interaction of ethanol with drug, hepatotoxic agent, carcinogen and vitamins. Alcoholism., 25, 157-17I
  57. Videla, L.A., Fernandez, V., Valenzuela, A. and Ugarte, G. (1980) The effect of chronic alcohol on glutathione concenturation in isolated hepatocytes. Biochem. J., 188, 549-562 https://doi.org/10.1042/bj1880549