Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Partial Purification and Characterization of an Extracellular Protease from Xenorhabdus nematophilus a Symbiotic Bacterium Isolated from an Entomopathogenic Nematode, Steinernema glaseri

  • Chae Young-Rae (School of Chemical Engineering and Bioengineering, University of Ulsan) ;
  • Ryu Keun-Garp (School of Chemical Engineering and Bioengineering, University of Ulsan)
  • Published : 2004.10.01
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Abstract

Entomopathogenic nematodes are used for insect control. Herein, an extracellular protease was partially purified from a culture supernatant of Xenorhabdus nematophilus, a symbiotic bacterium of an entomopathogenic nematode, Steinernema glaseri: using precipitation with $80\%$ v/v isopropyl alcohol followed by gel permeation chromatography with a packed Sephacryl S-300 HR media. The partially purified protease exhibited maximal activity at pH 7 in the presence of 1 mM $CaCl_2$. The protease was identified as a metallo-protease based on the inhibition of its activity by the metal chelating agent, EDTA.

Keywords

References

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