Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Comparing the Performance of One-column Process and Four-zone Simulated Moving Bed by Computer Simulation

  • Kim Young Sik (Department of Biological Engineering, ERC for Advanced Bioseparation Technology, Inha University) ;
  • Lee Chong Ho (Department of Biological Engineering, ERC for Advanced Bioseparation Technology, Inha University) ;
  • Wankat Phillip C. (School of Chemical Engineering, Purdue University) ;
  • Koon Yoon Mo (Department of Biological Engineering, ERC for Advanced Bioseparation Technology, Inha University)
  • Published : 2004.10.01
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Abstract

A new one-column chromatography process, analogous to a four-zone simulated moving bed (SMB), was presented. The basic principle of the process was identical to that of a four-zone SMB. The process consisted of one chromatographic column and four tanks, instead of the four columns in the four-zone SMB (1-1-1-1), and has been used for the separation of two amino acids, phenylalanine and tryptophan, using an ion exchange resin. The operating parameters for the one-column process and four-zone SMB were obtained from equilibrium theory. Computer simulations were used to compare the performances of the new one column process to that of the general four-zone SMB, using Aspen $Chromatography^{TM}$ v 11.1. The differences between the one-column and SMB processes in terms of the purities and yields of phenylalanine and tryptophan were less than 4 and about $6\%$, respectively. The lower purities of the one-column process were due to the loss of the developed concentration profiles in the column when the liquid was stored in tanks. The one-column process gave great flexibility, and would be useful for reconstructing an existing conventional chromatography process to one of a SMB.

Keywords

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