Korean Journal of Environmental Agriculture (한국환경농학회지)

The Korean Society of Environmental Agriculture (한국환경농학회)
권호 표지
DOI QR코드

DOI QR Code

Partial Reduction of Dinitroaniline Herbicide Pendimethalin by Bacillus sp. MS202

Bacillus sp. MS202에 의한 Dinitroaniline계 제초제인 Pendimethalin의 부분환원

  • Lee, Young-Keun (Radiation Application Research Division, Korea Atomic Energy Research Institute) ;
  • Chang, Hwa-Hyoung (Radiation Application Research Division, Korea Atomic Energy Research Institute) ;
  • Jang, Yu-Sin (Radiation Application Research Division, Korea Atomic Energy Research Institute) ;
  • Hyung, Seok-Won (Radiation Application Research Division, Korea Atomic Energy Research Institute) ;
  • Chung, Hye-Young (Radiation Application Research Division, Korea Atomic Energy Research Institute)
  • 이영근 (한국원자력연구소, 방사선이용연구부) ;
  • 장화형 (한국원자력연구소, 방사선이용연구부) ;
  • 장유신 (한국원자력연구소, 방사선이용연구부) ;
  • 형석원 (한국원자력연구소, 방사선이용연구부) ;
  • 정혜영 (한국원자력연구소, 방사선이용연구부)
  • Published : 2004.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

The persistence of pendimethalin in soil and ground water has an injurious effect on ecosystem. Pendimethalin-degrading bacterium was isolated from Masan, Gyeongnam province and temporarily identified as Bacillus sp. MS202 by the analysis of API CHB50, kit, FAME, and 16S rDNA sequence. from the analysis of pnedimethalin metabolite using TLC, GC, and GC-MS, we found that the degradation of pendimethalin by Bacillus sp. MS202 did not result in the dealkylated form, but the formation of the reduced compound, 6-amino-2-nitro-N(1-ethylpropyl)-3,4-xylidine or 2- amino-6-nitro-N(1-ethylpropyl)-3,4-xylidine.

토양과 지하수에서 pendimethalin의 지속성은 환경에 해로운 영향을 미친다. 경남 마산에서 분리한 pendimealalin분해 균주는 API CHB50 kit 시험, FAME분석, 그리고 16S rDNA 염기서열분석 결과로 Bacillus sp. MS202로 잠정적으로 동정하였다. TLC, GC, 그리고 GC-MS 분석에 의해 Bacillus sp. MS202가 pendimethalin의 $-NO_2$를 $-NH_2$로 환원시킨다는 것을 알 수 있었다. 이는 일반적으로 알려진 호기성 미생물에 의한 pendimethalin 분해가 탈알킬화가 우선한다는 보고와 상반되는 새로운 결과이다.

Keywords

References

  1. Tomlin, C. D. S. (2003) The pesticide Manual, 13th (ed), Bntish Crop Protection Council, Bracknell, UK, p.752-753
  2. Walker, A. and Bond, W. (1977) Persistence of the herbicide AC 92,553, N-(1-ethypropy1)-2,6-dinitro-3,4-xylidine in soils, Pestic. Sci. 8, 359-369 https://doi.org/10.1002/ps.2780080409
  3. Garcia-Valcarcel, A. I. and Tadeo, J. L. (2003) Influence of organic fertilizer application on pendirnethalm volatilization and persistence in soil, J. Agric. Food Chem. 51, 999-1004 https://doi.org/10.1021/jf0205561
  4. Parochetti, J. V. and Dec, G. W. J. (1978) Photo-degradation of eleven dinitroaniline herbiddes, Weed Sci. 26, 153-156
  5. Kiinmo, A. M, Arto, J. S. and Jussi, V. K. K (2003) Bioaccuinulation and toxicity of sediment associated herbicides (ioxynil, pendimethalm, and bentazone) in Lumbricutus variegatus (Oligcchaeta) and Chirwtomus riparius (Insecta), Ecotoxicol. Environm. Safety, 56, 398-410 https://doi.org/10.1016/S0147-6513(03)00010-1
  6. US EPA (1999) Persistent bioaccumulative toxic (PBT) chemicals, Final Rule, Fed. Regist, 64, 58666-58753
  7. lesce, M. R, Graziano, M. L., Germola, F., Montella, S., di, Gioia, L., and Stasio, C. (2003) Effects of sensitizers on the photodegradadon of the systemic fungicide tnadimenol, Chemosphere, 51, 163-166 https://doi.org/10.1016/S0045-6535(02)00823-8
  8. Polonca, T. and Iztok, A. (2003) degradation of org-anophosphorus compounds by X-ray irradiation, Radiat. Phys. Oiem, 67, 527-530 https://doi.org/10.1016/S0969-806X(03)00099-9
  9. Chen, W. and Mulchandard, A (1998) The use of live bio-catalysts for pesticide detoxification, Trends biotechnol. 16, 71-76 https://doi.org/10.1016/S0167-7799(97)01160-8
  10. Engelhardt, G, Wallnofer, P. R., and Flapp, R (1973) Purification and properties of an aryl acylairddase of BaciItus sphaericus, catalyzing the hydrolysis of various phenylamide herbicides and fungicides, Appl. Microbiol. 26, 709-710
  11. Bachofer, R. and Lingens, M. (1983) Degradation of carboxanilide fungicide by a Nocardia species, Phusiol. Chem. 364, 21-29 https://doi.org/10.1515/bchm2.1983.364.1.21
  12. Blake, J. and Kaufman, D. D. (1975) Characterization of acylamide-hydrolyzing enzymes from Fusarium oxysporum Schlecht, Pestic. Biochem. Physiot. 5, 305-313 https://doi.org/10.1016/0048-3575(75)90049-8
  13. Lee, S. J., Katayama, A., and Kimura, M. (1995) Microbial degradation of paraquat sorbed to plant residues, J. Avric. Food Chem. 19, 291-293
  14. Alexander, M (1980) Biodegradation of chemicals of envrronmental concern, Science, 211, 132-138 https://doi.org/10.1126/science.7444456
  15. Parr, J. F. and Snith, S. (1976) Degradation of toxaphene in selected anaerobic soil environment, Soil Sci. 121, 52-57 https://doi.org/10.1097/00010694-197601000-00009
  16. Singh, S. B. and Kulshrestha, G. (1991) Microbial degradation of pendimethalin, J. Environ. Sci. Heidth, B26, 309-321
  17. Parr, J. F. and Smith, S. (1973) Degradation of trifluralin under laboratory conditions and soil anaerohiosis, Soil Sci. 115, 55-63 https://doi.org/10.1097/00010694-197301000-00008
  18. Raphi, T. M, Deborah, L. A., and Lawrencew, P. W. (1995) Isolation and characterization of a Pseudomonas sp. that mineralizes the s-Triazine herbicede Atrazme, Appl. Environ. Microb., 61, 1451-1457
  19. Julian, R. M, Takuichi, S., Andrew J. W., Tracey, A. M, John C. F., Sarah, J. K, and William, G. W. (1998) Design and evaluation of useful bacterium-specific PCR primers that amplify genes coding for bacteria 16S rRNA, Appl. Environ. Mcrob., 64, 795-799
  20. Franco, C D., Beccari, E., Santini, T., Hsaneschi, G., and Tecce, G. (2002) Colony shape as a genetic trait in the pattem-forming Bacillus mycoides, BMC Microbiol. 2, 1471-2180
  21. Gita, K., Shashi, B. S., Shashi, P. L, and Nanjapur, T. Y. (2000) Effect of long-term application of pendi-methalin: enhanced degradation in soil, Pest Manag. Sci. 56, 202-206 https://doi.org/10.1002/(SICI)1526-4998(200002)56:2<202::AID-PS97>3.0.CO;2-C
  22. Joseph, S. (2003) Recent advances in the thin-layer chrornatography of pesticides: a review, J. AOAC Int. 86, 602-611
  23. Kulshrestha, G. and Singh, S. B. (1992) Influence of soil moisture and microbial activity on pendimethalin degradation, Bull. Enuiron. Contam. Toxicol. 48, 269-274
  24. Kole, R. K., Saha, J., Pal, S. Chaudhuri, S., and Chowdhury, A. (1994) Bacterial degradation of the herbicide pendimethalin and activity evaluation of its metabolites, Bull. Enoiron. Contam. Toxicol. 52, 779-786