Korean Journal of Environmental Agriculture (한국환경농학회지)

The Korean Society of Environmental Agriculture (한국환경농학회)
권호 표지
DOI QR코드

DOI QR Code

Culture Optimization for Bacillus lentimorbus G-74 by Using a Miscanthus purpurascens Juice Medium

억새즙액 배지를 이용한 Bacillus lentimorbus G-74 균주의 배양 최적화

  • Kang, Sun-Chul (Division of Food, Biological and Chemical Engineering, Daegu University) ;
  • Seo, Hae-Jeong (Division of Food, Biological and Chemical Engineering, Daegu University)
  • 강선철 (대구대학교 식품생명화학공학부) ;
  • 서해정 (대구대학교 식품생명화학공학부)
  • Published : 2004.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

Miscanthus purpurascens Juice containing potassium (37,952 mg/L), nitrogen (14,000 mg/L), phosphorus (6,800 mg/L), magnesium (5,969 mg/L), calcium (5,910 mg/L), etc., was investigated to develop a novel meidum far the mass cultivation of useful microorganisms. For this research, we first isolated an antagonistic bacterium G-74 from soil, which showed strong growth inhibition against two phytopathogenic fungi, Rhizoctonia solani and Botrytis cinerea, and identified as Bacillus lentimorbus G-74 based on the morphological characteristics and MIDI analysis. Culture conditions for G-74 strain in the M. purpurascens juice medium were optimized. Dilution rate of the medium, temperature and initial pH for the optimum growth of G-74 strain were 30% (V/V), $35^{\circ}C$ and 5.0, respectively. It was found that additions of 2.0% (W/V) corn starch as a carbon source and 1.0% (W/V) yeast extract as a nitrogen source in this medium increased B. lentimorbus G-74 growth to 66% more efficient than Luria Bertani medium.

억새즙액을 이용하여 유용한 미생물의 대량배양을 위한 새로운 배지를 개발하고자 본 연구를 수행하였다. 억새즙액의 성분을 조사한 결과 칼륨 (37,952 mg/L), 질소 (14,000 mg/L), 인산 (6,800mg/L), 마그네슘 (5,969 mg/L), 칼슘 (5,910 mg/L) 등이 주성분을 차지하였다. 본 연구를 위하여 먼저 식물병원성균인 뿌리썩음병 원인균인 Rhizoctonia solani, 잿빛곰팡이 병을 일으키는 Botrytis cinerea에 대하여 강력한 저해 활성을 갖는 길항균 G-74를 토양으로부터 분리하였으며, 이 균의 형태관찰과 MIDI 분석을 통하여 Bacillus lentimorbus G-74 균주로 최종 동정하였다. 이 균의 억새즙액 배지에 대한 최적 배양조건을 조사한 결과 30% (V/V) 희석배지, $35^{\circ}C$ 배양온도, 5.0의 초기 배지 pH에서 가장 좋은 성장을 기록하였다. 또한 이 배지에 탄소원으로서 2.0% (W/V)의 corn starch 및 질소 원으로서 1.0% (W/V)의 yeast extract를 추가적으로 첨가하면 최상의 성장을 보여주었으며, 이 경우 대조구인 LB 배지보다도 균체성장이 66% 이상 증가하였다.

Keywords

References

  1. Hwang, B. K. (1985) Plant Medicine, Tamgu Publisher, Seoul, Korea
  2. Mandeel, Q. and Baker, R. (1993) Mechanisms involved in biological control of Fusarium wilt of cucumber with strains of nonpathogenic Fusarium oxysporium, Phytopathol. 81, 462-469
  3. Paulitz, T. C., Park, S. and Baker, R. (1987) Biological control of Fusariwn wilt of cucumber with nonpathogenic isolates of Fusarium oxysporum, Can. J. Mcrobiol. 33, 349-353 https://doi.org/10.1139/m87-061
  4. Sutton, J. C. and Peng. G. (1993) Biocontrol of Botrytis cinerea in strawberry leaves, Phytopathol. 83, 615-621 https://doi.org/10.1094/Phyto-83-615
  5. Burpee, L. L. and Goulty, L. G. (1984) Evaluations of fungicides for control of pink and gray snow mold on creeping bentgrass, In R. W. Sheard (ed). Turfgrass Research Annual Report, Univ. of Guelph, Canada, p.6-7
  6. Omura, S., Tomoda, H., Kimura, K., Zhen, D. -Z, Kumagai, H., Igarashi, K., Imamura, N., Takahashi, Y., Tanaka, Y. and Iwai, Y. (1988) Atpenins, new antifungal antibiotics produced by Penicillium sp. (production, isolation, physico-chemical and biological properties), J. Antibiot. 41, 1769-1773 https://doi.org/10.7164/antibiotics.41.1769
  7. Yoshie, Y., Katsushige, I., Yoshihisa, U., Atsuko, O., Kazutoh, T., Ikunoshin, K and Hiroshin, N. (1993) Isolation, structures, and antifungal activities of new Aureobasidins, J. Antibiot. 46, 1347-1354 https://doi.org/10.7164/antibiotics.46.1347
  8. Baker, R. (1968) Mechanisms of biological control of soilborn plant pathogens, Ann. Rev. Phytopathol. 6, 263-294 https://doi.org/10.1146/annurev.py.06.090168.001403
  9. Lewandowski, I., Oifton-Brown, J. C., Scurlock, J. M. O. and Huisman, W. (2000) Miscanthus: European experience with a novel energy crop, Biomass Bioenerg. 19, 209-227 https://doi.org/10.1016/S0961-9534(00)00032-5
  10. Greef, J. M. and Deuter, M. (1993) Syntaxonomy of Miscanthus giganteus Greef et. Deu, Angeuxmdte Botanik. 67, 87-90
  11. Cho, M. K. (2000) Screening of competent intestinal microorganisms in poultry for the development of probiotics. MS. thesis, Chungbuk National University, Cheongju, Korea
  12. Kim, C. H., Kim, T. J. and Hong, S. I. (1990) The medium optimization through continuous culture of an methanol utilizing bacterium for SCP prodution, Kor. J. Appl. Microbiol. Biotechnol. 5, 355-363
  13. Pridham, T. G, Hall, H H and Jackson, R W (1965) Effect of antimicrobial agents on the milky disease bacteria Bacillus popilliae and Bacillus lentimorbus, Appl. McrobioI. 13, 1000-1004
  14. Assoc. of Korea Food Industrial (1991) Food Industrial Dictionary: Minerals, Hanil Publisher, Seoul, Korea, p.853-858
  15. Kang, M R, Lee, I. H, Jun, H, Kim, Y. S. and Kim, S. C (2001) Elemental analysis in Astragli radix by using ICP-AES and determination of the original agricultural place of oriental medicine by using a chemometrics, Kor. Anal. Sci. Teelmol. 14, 316-321
  16. Lee, J. S., Park, S. and Park, G S. (1989) Optimization of media composition and cultivation for the mycelial growth of Agrocybe cylindracea, Kor. J. Food Sci. Technol. 21, 399-403
  17. Son, J. Y. (1993) Animal waste treatment and utilization, J. Bio. Fac. Eng. 2, 150-160
  18. Lee, Y., Joo, W. H. and Sea, J. Y. (1995) Decentralized composting of garbage in a small composter for dwelling house, Kor. J. Environ. Agric. 14, 345-350
  19. Sung, N. K., Yon, H. D., Shim, K. H. and Lee, C. S. (1977) Studies on the fennentative utilization of cellulosic wastes; culture of celluloytic bacteria from Mscanthus, Kor. J. Appl. Mcrobiol. Bioeng. 5,127-131
  20. Bae, M., Lee, K. J. and Kho, Y. H. (1977) Studies on microbial utilization of agricultural wastes, Kor. J. Appl. Microbiol. Bioeng. 5, 119-125
  21. Dorta, B., Bosch A, Areas, J. A. and Ertola, R. J. (1990) High level of sporulation of Metarhizium anisopliae in a medium containing by-products, Appl. Mcrobiol. Biotechnol. 33, 712-715 https://doi.org/10.1007/BF00604944
  22. Ha, Y. M. (1989) Culture conditions of Scenedesmus dimorphus in waste water from the biodegradation of cabbages, MS. thesis, Inha University, Incheon, Korea
  23. Lee, N. S and Kyung, K H (1992) Production of Candida utilis biomass on Chinese cabbage juice, Korean J. Food Sci. Technol. 24, 221-225
  24. Moorthy, V. K. and Mohanan, R C (1991) Mass production of the entomopathogen Metarhizium anisopliae in coconut water wasted from copra making industry, J. Plantation Crop. 19, 54-69
  25. Paik, H. D., Lee, S. H, Hwang, S. K. and Oh, Y. J. (1988) Optimization of media composition and cultural conditions for $\delta$-endotoxin production by Bacillus thuringiensis var. israelensis, Kor. J. Appl. Mcrobiol. Bioeng. 16, 11-16
  26. Moon, K. H., Kim, P. H, Yoon, J. W, Sung, J. M. and Kim, S. W. (1997) Optimization of production medium for mass culture of Beauveria sp. C208, Kor. J. Appl. Microbial. Biotechnol. 25, 606-611
  27. Park, K. S. and Lee, J. S. (1991) Optimization of media composition and culture conditions for the mycelial growth of Coriolus versicolor and Lentinus edodes, Kor. J. Biotechnol. Bioeng. 6, 91-98
  28. Yun, J. W., Kang, S. C. and Song, S. G. (1996) Microbial transformation on fructose to mannitol by Lactobacillus sp. KY-107, Biotechnol. Lett. 18, 35-40 https://doi.org/10.1007/BF00137807
  29. Yun, J. W., Kang, S. C. and Song, S. G. (1996) Effect of nitrogen sources on the production of polyols by Aureobasidium pullulans, J. Microbiol. Biotechnol. 6, 60-62