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The Korean Association of Immunobiologists (대한면역학회)
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Characterization of a Monoclonal Antibody Specific to Human Siah-1 Interacting Protein

인체 SIP 단백질에 특이적인 단일클론 항체의 특성

  • Yoon, Sun Young (Laboratory of Cell Biology, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Joo, Jong Hyuck (Laboratory of Cell Biology, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Kim, Joo Heon (Department of Pathology, Eulji University School of Medicine) ;
  • Kang, Ho Bum (Laboratory of Cell Biology, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Kim, Jin Sook (Laboratory of Cell Biology, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Lee, Younghee (Laboratory of Cell Biology, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Kwon, Do Hwan (Laboratory of Cell Biology, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Kim, Chang Nam (Department of Surgery, Eulji University School of Medicine) ;
  • Choe, In Seong (Laboratory of Cell Biology, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Kim, Jae Wha (Laboratory of Cell Biology, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
  • 윤선영 (한국생명공학연구원 세포생물학연구실) ;
  • 주종혁 (한국생명공학연구원 세포생물학연구실) ;
  • 김주헌 (을지의과대학 병리학교실) ;
  • 강호범 (한국생명공학연구원 세포생물학연구실) ;
  • 김진숙 (한국생명공학연구원 세포생물학연구실) ;
  • 이영희 (한국생명공학연구원 세포생물학연구실) ;
  • 권두한 (한국생명공학연구원 세포생물학연구실) ;
  • 김창남 (을지의과대학 외과학교실) ;
  • 최인성 (한국생명공학연구원 세포생물학연구실) ;
  • 김재화 (한국생명공학연구원 세포생물학연구실)
  • Published : 2004.03.31
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Abstract

Background: A human orthologue of mouse S100A6-binding protein (CacyBP), Siah-1-interacting protein (SIP) had been shown to be a component of novel ubiquitinylation pathway regulating $\beta$-catenin degradation. The role of the protein seems to be important in cell proliferation and cancer evolution but the expression pattern of SIP in actively dividing cancer tissues has not been known. For the elucidation of the role of SIP protein in carcinogenesis, it is essential to produce monoclonal antibodies specific to the protein. Methods: cDNA sequence coding for ORF region of human SIP gene was amplified and cloned into an expression vector to produce His-tag fusion protein. Recombinant SIP protein and monoclonal antibody to the protein were produced. The N-terminal specificity of anti-SIP monoclonal antibody was conformed by immunoblot analysis and enzyme linked immunosorbent assay (ELISA). To study the relation between SIP and colon carcinogenesis, the presence of SIP protein in colon carcinoma tissues was visualized by immunostaining using the monoclonal antibody produced in this study. Results: His-tag-SIP (NSIP) recombinant protein was produced and purified. A monoclonal antibody (Korea patent pending; #2003-45296) to the protein was produced and employed to analyze the expression pattern of SIP in colon carcinoma tissues. Conclusion: The data suggested that anti-SIP monoclonal antibody produced here was valuable for the diagnosis of colon carcinoma and elucidation of the mechanism of colon carcinogenesis.

Keywords

Acknowledgement

Supported by : 한국생명공학연구원

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