Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
권호 표지
DOI QR코드

DOI QR Code

Mapping of Quantitative Trait Loci on Porcine Chromosome 7 Using Combined Data Analysis

  • Zuo, B. (Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture, College of Animal Science and Veterinary Medicine, Huazhong Agriculture University) ;
  • Xiong, Y.Z. (Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture, College of Animal Science and Veterinary Medicine, Huazhong Agriculture University) ;
  • Su, Y.H. (Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture, College of Animal Science and Veterinary Medicine, Huazhong Agriculture University) ;
  • Deng, C.Y. (Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture, College of Animal Science and Veterinary Medicine, Huazhong Agriculture University) ;
  • Lei, M.G. (Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture, College of Animal Science and Veterinary Medicine, Huazhong Agriculture University) ;
  • Zheng, R. (Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture, College of Animal Science and Veterinary Medicine, Huazhong Agriculture University) ;
  • Jiang, S.W. (Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture, College of Animal Science and Veterinary Medicine, Huazhong Agriculture University) ;
  • Li, F.E. (Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture, College of Animal Science and Veterinary Medicine, Huazhong Agriculture University)
  • Received : 2003.11.21
  • Accepted : 2004.05.15
  • Published : 2004.10.01
Download PDF
⟨ Previous Next ⟩

Abstract

To further investigate the regions on porcine chromosome 7 that are responsible for economically important traits, phenotypic data from a total of 287 F2 individuals were collected and analyzed from 1998 to 2000. All animals were genotyped for eight microsatellite loci spanning the length of chromosome 7. QTL analysis was performed using interval mapping under the line-cross model. A permutation test was used to establish significance levels associated with QTL effects. Observed QTL effects were (chromosomewide significance, position of maximum significance in centimorgans): Birth weight (<0.01, 3); Carcass length (<0.05, 80); Longissimus muscle area (<0.01, 69); Skin percentage (<0.01, 69); Bone percentage (<0.01, 74); Fat depths at shoulder (<0.05, 54);Mean fat depth (<0.05, 81); Moisture in m. Longissimus Dorsi (<0.05, 88). Additional evidence was also found which suggested QTL for dressing percentage and fat depths at buttock. This study offers confirmation of several QTL affecting growth and carcass traits on SSC7 and provides an important step in the search for the actual major genes involved in the traits of economic interest.

Keywords

References

  1. Andersson-Eklund, L., L. Marklund, K. Lundstrom, C. S. Haley, K. Andersson, I. Hansson, M. Moller and L. Andersson. 1998. Mapping quantitative trait loci for carcass and meat traits in a Wild boar${\times}$Large White intercross. J. Anim. Sci. 76:694-700.
  2. Darvasi, A., A. Weinreb, V. Minke, J. I. Weller and M. Soller. 1993. Detecting marker-QTL linkage and estimating QTL gene effect and map position using a saturated genetic map. Genetics 134:943-951.
  3. de Koning, D. J., L. L. G. Janss, A. P. Rattink, P. A. M. van Oers, B. J. de Vries, M. A. M. Groenen, J. J. van der Poel, P. N. de Groot, E. W. Brascamp and J. A. M. van Arendonk. 1999. Detection of quantitative trait loci for back fat thickness and intramuscular fat content in pigs. Genetics 152:1679-1690.
  4. de Koning, D. J., A. P. Rattink, B. Harlizius, J. A. van Arendonk, E. W. Brascamp and M. A. Groenen. 2000. Genome-wide scan for body composition in pigs reveals important role of imprinting. Proc. Natl. Acad. Sci. USA. 97:7947-7950.
  5. de Koning, D. J., A. P. Rattink, B. Harlizius, M. A. M. Groenen, E. W. Brascamp and J. A. M. van Arendonk. 2001. Detection and characterization of quantitative trait loci for growth and reproduction traits in pigs. Livest Prod Sci. 72:185-198.
  6. Genet, C., C. Renard, C. Cabau, C. Rogel-Gaillard, J. Gellin and D. Milan. 2001. In the QTL region surrounding porcine MHC, gene order is conserved with human genome. Mamm. Genome. 12:246-249.
  7. Green, P., K. Falls and S. Crooks. 1990. Document for CRIMAP, version 2.4. Washington Uni. School of Medicine. St. Louis, MO.
  8. Haley, C. 1999. Advances in Quantitative Trait Locus Mapping. AgBiotechNet at URL http://agbio.cabweb.org
  9. Lee, H. K., S. S. Lee, T. H. Kim, G. J. Jeon, H. W. Jung, Y. S. Shin, J. Y. Han, B. H. Choi and I. C. Cheong. 2003. Detection of imprinted quantitative trait locifor growth traits in pigs. Asian-Aust. J. Anim. Sci. 16:1087-1092.
  10. Malek, M., J. C. M. Dekkers, H. K. Lee, T. J. Bass and M. F. Rothchild. 2001a. A molecular genome scan analysis to identify chromosomal regions influencing economic traits in the pig. I. Growth and body composition. Mamm. Genome. 12:630-636.
  11. Malek, M., J. C. M. Dekkers, H. K. Lee, T. J. Bass, K. Prusa, E. Huff-Lonergan and M. F. Rothschild. 2001b. A molecular genome scan analysis to identify chromosomal regions influencing economic traits in the pig. II. Meat and muscle composition. Mamm. Genome. 12:637-645.
  12. Milan, D., J. P. Bidanal, P. Le Roy, C. Chevalet, N. Woloszyn, J. C. Caritez, J. Gruand, H. Lagant, M. Bonneau, L. Lefaucheur, C. Renard, M. Vaiman, P. Mormede, C. Desautes, Y. Amigues, F. Bourgeois, J. Gelin and L. Ollivier. 1998. Current status of QTL detection in Large White$\times$Meishan crosses in France. Proc 6th World Congr Genet Appl Livest Prod. 26:414-417.
  13. Milan, D., J. P. Bidanel, N. Iannuccelli, J. Riquet, Y. Amigues, J. Gruand, P. Le Roy, C. Renard and C. Chevalet. 2002. Detection of quantitative trait loci for carcass composition traits in pigs. Genet. Sel. Evol. 34(6):705-728.
  14. Moser, G., E. Mueller, P. Beeckmann, G. Yue and H. Geldermenn. 1998. Mapping of QTLs in F2 generations of Wild Boar, Pietrain and Meishan pig. Proc 6th World Congr Genet Appl Livest Prod. 26:478-481.
  15. Ovilo, C., M. Perez-Enciso, C. Barragan, A. Clop, C. Rodriguez, M. A. Oliver, M. A. Toro and J. L. Noguera. 2000. A QTL for intramuscular fat and backfat thickness is located on porcine chromosome 6. Mamm. Genome. 11:344-340.
  16. Ovilo, C., A. Clop, J. L. Noguera, M. A. Oliver, C. Barragan, C. Rodriguez, L. Silio, M. A. Toro, A. Coll, J. M. Folch, A. Sanchez, D. Babot, L. Varona and M. Perez-Enciso. 2002. Quantitative trait locus mapping for meat quality traits in an Iberian$\times$Landrace F2 pig population. J. Anim. Sci. 80(11):2801-2808.
  17. Olivier, D., C. Renard, M. Yerle, T. Faraut, J. Riquet, A. Robic, T. Schiex, A. Rink and D. Milan. 2003. Rearranged gene order between pig and human in a QTL region on SSC 7. Mamm. Genome. 14:71-80.
  18. Rattink, A. P., D. J. De Koning, M. Faivre, B. Harlizius, J. A. M. van Arendonk and M. A. M. Groenen. 2000. Fine mapping and imprinting analysis for fatness trait QTLs in pigs. Mamm. Genome. 11:656-661.
  19. Rohrer, G. A. and J. Keele. 1998a. Identification of quantitative trait loci affecting carcass composition in swine I. Fat deposition traits. J. Anim. Sci. 76:2247-2254.
  20. Rohrer, G. A. and J. Keele. 1998b. Identification of quantitative trait loci affecting carcass composition in swine II. Muscling and wholesale product yield traits. J. Anim. Sci. 76:2255-2262.
  21. Rothschild, M. F., H. C. Liu, C. K. Tuggle, T. P. Yu and L. Wang. 1995. Analysis of chromosome 7 genetic markers for growth and carcass performance traits. J. Anim. Breed Genet. 112:341-348.
  22. Sato, S., Y. Oyamada, K. Atsuji, T. Nade, S. Sato, E. Kobayashi, T. Mitsuhashi, K. Nirasawa, A. Komatsuda, Y. Saito, S. Terai, T. Hayashi and Y. Sugimoto. 2003. Quantitative trait loci analysis for growth and carcass traits in a eishan$\times$Duroc F2 resource population. J. Anim. Sci. 81(12):2938-2949.
  23. Su, Y. H., Y. Z. Xiong, Q. Zhang, S. W. Jiang, L. Yu, M. G. Lei, R. Zheng and C. Y. Deng. 2002a. Detection of quantitative trait loci for growth in Large White$\times$Meishan intercross. Acta Genetica Sinica (China). 29:607-611.
  24. Su, Y. H., Y. Z. Xiong, Q. Zhang, S. W. Jiang, M. G. Lei, L. Yu, R. Zheng and C. Y. Deng. 2002b. Mapping quantitative trait loci for fat deposition in carcass in pigs. Acta. Genetica Sinica (China). 29:681-684.
  25. Wada, Y., T. Akita, T. Awata, T. Furukawa, N. Sugai, Y. Inage, K. Ishii, Y. Ito, E. Kobayashi, H. Kusumoto, T. Matsumoto, S. Mikawa, M. Miyake, A. Murase, S. Shimanuki, T. Sugiyama, Y. Uchida, S. Yanai and H. Yasue. 2000. Quantitative trait loci analysis in Meishan${\times}$Gottingen cross population. Anim. Genet. 31:376-384.
  26. Xiong, Y. Z. and C. Y. Deng. 1999. Principle and method of swine testing. Chinese Agricultural Press, Beijing.
  27. Zuo, B., Y. Z. Xiong, Y. H. Su, C. Y. Deng, R. Zheng, M. G. Lei and L. Yu. 2003a. Mapping and genetic effect analysis of swine quantitative trait loci using 24 microsatellites. Acta Veterinaria et Zootechnica Sinica (China). 34:139-146.
  28. Zuo, B., Y. Z. Xiong, Y. H. Su, C.Y. Deng, R. Zheng and S. W. Jiang. 2003b. Mapping Quantitative Trait Loci for Meat Quality on Pig Chromosome 3, 4 and 7. Asian-Aust. J. Anim. Sci. 16:320-324.

Cited by

  1. gene vol.126, pp.4, 2009, https://doi.org/10.1111/j.1439-0388.2008.00740.x
  2. The Functional SNPs in the 5’ Regulatory Region of the Porcine PPARD Gene Have Significant Association with Fat Deposition Traits vol.10, pp.11, 2015, https://doi.org/10.1371/journal.pone.0143734
  3. Molecular Cloning and Characterization of Bovine HMGA1 Gene vol.20, pp.11, 2004, https://doi.org/10.5713/ajas.2007.1662