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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Inhibition of Herpesvirus-6B RNA Replication by Short Interference RNAs

  • Yoon, Jong-Sub (Department of Microbiology, College of Medicine, The Catholic University of Korea) ;
  • Kim, Sun-Hwa (Department of Microbiology, College of Medicine, The Catholic University of Korea) ;
  • Shin, Min-Chul (Department of Microbiology, College of Medicine, The Catholic University of Korea) ;
  • Lee, Dong-Gun (Department of Internal Medicine, College of Medicine, The Catholic University of Korea) ;
  • Hong, Seong-Karp (Department of Microbiology, College of Medicine, The Catholic University of Korea) ;
  • Jung, Yong-Tae (Department of Microbiology, College of Advanced Science, Dankook University) ;
  • Khang, In-Gu (Department of Internal Medicine, College of Medicine, The Catholic University of Korea) ;
  • Shin, Wan-Shik (Department of Internal Medicine, College of Medicine, The Catholic University of Korea) ;
  • Kim, Chun-Choo (Department of Internal Medicine, College of Medicine, The Catholic University of Korea) ;
  • Paik, Soon-Young (Department of Microbiology, College of Medicine, The Catholic University of Korea)
  • Published : 2004.05.31
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Abstract

RNA interference (RNAi) is a process of sequence-specific gene silencing, which is initiated by double-stranded RNA (dsRNA). RNAi may also serve as an antiviral system in vertebrates. This study describes the inhibition of herpesvirus-6B (HHV-6B) replication by short interference RNAs (siRNAs) that are targeted to the U38 sequence that encodes DNA polymerase. When virus-infected SupT1 cells were treated by siRNA, these cells blocked the cytopathic effect (CPE) and detected the HHV-6B antibody-negative in indirect immunofluorescence assays (IFA). Our result suggests that RNAi can efficiently block Herpesvirus-6B replication.

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