Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Effects of Selenium, Copper and Magnesium on Antioxidant Enzymes and Lipid Peroxidation in Bovine Fluorosis

  • Han, Bo (College of Veterinary Medicine, China Agricultural University) ;
  • Yoon, Soonseek (College of Veterinary Medicine, Seoul National University) ;
  • Su, Jingliang (College of Veterinary Medicine, China Agricultural University) ;
  • Han, H.R. (College of Veterinary Medicine, Seoul National University) ;
  • Wang, Mei (College of Veterinary Medicine, China Agricultural University) ;
  • Qu, Weijie (College of Veterinary Medicine, China Agricultural University) ;
  • Zhong, Daibin (College of Veterinary Medicine, China Agricultural University)
  • Received : 2004.04.09
  • Accepted : 2004.08.26
  • Published : 2004.12.01
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Abstract

The antioxidant enzymes, lipid peroxidation and free radicals assessment were made of the effects of selenium, copper and magnesium on bovine endemic fluorosis under high fluoride, low selenium and low copper productive conditions. Thirty-two beef cattle were selected from high fluoride area, and randomly divided into four groups with eight cattle each as follows: (1) high fluoride control group (HFC); (2) supplemented group with 0.25 mg/kg selenium (HFSe); (3) supplemented group with 15 mg/kg copper (HFCu) and (4) supplemented group with 0.25 mg/kg selenium+15 mg/kg copper+1 mg/kg magnesium (HFSeCuMg) per day for 83 days. Moreover, eight beef cattle were selected from non-high fluoride area as normal control group. Blood samples were collected from cattle on 0 d, 30 d and 83 d respectively, to analyze the enzyme activities and concentration of GSH-px, CAT, SOD, MDA and free radicals. The results showed that the contents of free radicals and MDA in HFC group were significantly higher, and the whole blood GSH-px, CAT, erythrocyte SOD activities were lower than the normal control group. Free radicals, metabolic imbalance and antioxidant disorder therefore, play an important role in fluorosis. However, GSH-px, CAT and SOD activities in HFSe group and HFSeCuMg group at 30 d and 83 d were markedly higher than the same groups at the 0 d and the HFC group at the same time. Likewise, there was a corresponding reduction in the contents of free radicals and MDA. These findings indicated that supplementation with selenium, copper and magnesium elevated high fluoride bovine antioxidant enzymes, and decreased MDA and free radicals contents. But, the activities of supplementation selenium group did not increase until day 83. These results demonstrated that fluorosis was associated with lower serum Se and Cu levels than in the control, and it was therefore concluded that fluorosis is associated with decreased serum levels of these minerals. Long-term high fluoride intake under productive condition enhances oxidative stress in the blood, thereby disturbing the antioxidant defense of cattle. Increased oxidative stress could be one of the mediating factors in the pathogenesis of toxic manifestations of fluoride. It is benefical for high fluoride cattle supplemented with proper selenium, copper and magnesium to increase fluoride excretion and obtain the protective impact of the activity of oxidative enzymes, and to decrease lipid peroxidation and free radicals contents.

Keywords

References

  1. Han, B., M. Y. Li and Y. Shi. 2002. Studies on the toxicology of endemic fluorosis in cattle, XXII World Buiatrics Congress, Hannover, German, 8.
  2. Han, B. and Y. Shi. 1998. Studies on the etiology, pathogenesis and control of bovine endemic fluorosis. J. North. Agric. Univ. 29(3):260-270.
  3. Jubb, T. F., T. E. Annand, D. C. Main and G. M. Murphy. 1993. Phosphorus supplements and fluorosis in cattle-a Northern Australian experience. Aust. Vet. J. 70:379-83. https://doi.org/10.1111/j.1751-0813.1993.tb00818.x
  4. Wei, Z. D. and Y. Wei. 2002. Fluoridation in China: a clouded future. Fluoride 35(1):1-4.
  5. Wang, A. G., T. Xia, R. Ru, J. Yuan, X. M. Chen and K. D. Yang. 2004. Antagonistic effect of selenium on oxidative stress, DNA damage, and apoptosis induced by fluoride in human hepatocytes. Fluoride 37(2):107-110.
  6. Chlubek, D. 2003. Fluoride and oxidative stress. Fluoride 36(4):217-228.
  7. Myśliwiec, Z. and A. Machoy-Mokrzyńska. 2002. Effects of selenium on serum lipids and enzyme activities in fluorideintoxicated rats. Fluoride 35(3):168-175.
  8. Reddy, G. B., A. L. Khandare, P. Y. Reddy, G. S. Rao, N. Balakrishna and I. Srivalli. 2003. Antioxidant defense system and lipid peroxidation in patients with skeletal fluorosis and in fluoride-intoxicated rabbits. Toxicol. Sci. 72(2):363-368.
  9. Fujihara, T., C. Hosoda and T. Matsui. 1995. Mineral status of grazing sheep in the dry area of mid land china. Asian-Aust. J. Anim. Sci. 8(2):236-240.
  10. Naresh, R., S. K. Dwivedi, S. Dey and D. Swarup. 2001. Zinc, copper and cobalt concentrations in blood during Inflammation of the mammary gland in dairy cows. Asian-Aust. J. Anim. Sci. 14(4):559-563.
  11. Meral, I., H. Demir, H. Gunduz, N. Mert and I. Dogan. 2004. Serum copper, zinc, manganese and magnesium status of subjects with chronic fluorosis. Fluoride 37(2):102-105.
  12. Gowda, N. K. S., C. S. Prasad, L. B. Ashok and J. V. Ramana. 2004. Utilization of dietary nutrients, retention and plasma level of certain minerals in crossbred dairy cows as influenced by source of mineral supplementation. Asian-Aust. J. Anim. Sci. 17(2):221-227.
  13. Han, B., D. B. Zhong, P. F. Wu, W. J. Qu and M. Wang. 2004. Effect of excessive fluoride environment on productivity and reproductive performance in dairy cows. XXⅢ World Buiatrics Congress, Quebec, Canada, 7.
  14. Shivarajashankara, Y. M., A. R. Shivashankara, P. G. Bhat and S. H. Rao. 2003. Lipid peroxidation and antioxidant systems in the blood of young rats subjected to chronic fluoride toxicity. Indian J. Exp. Biol. 41(8):857-860.
  15. Kapoor, V., T. Prasad and V. K. Paliwal. 2001. Blood biochemical constituents in calves following subclinical levels of fluoride toxicosis. Fluoride 34(2):126-131.
  16. Chinoy, N. J. and T. N. Patel. 2000. The influence of fluoride and/or aluminum on free radical toxicity in the brain of female mice and beneficial effects of some antidotes. Fluoride 33:S8.
  17. Chinoy, N. J. 2003. Fluoride stress on antioxidant defence systems. Fluoride 36(3):138-141.
  18. Lawson, P. B. and M. H. Yu. 2003. Fluoride inhibition of superoxide dismutase (SOD) from the earthworm Eisenia fetida. Fluoride 36(3):143-151.
  19. Patel, D. and N. J. Chinoy. 1998. Influence of fluoride on biological free radical reactions in ovary of mice and its reversal. Fluoride 31(3):S27.
  20. Pang, Y. X., Y. Q. Guo, P. Zhu, K. W. Fu, Y. F. Sun and R. Q. Tang. 1996. The effects on fluoride, alone and in combination with selenium, on the morphology and histochemistry of skeletal muscle. Fluoride 29(1):59-62.
  21. Choi, Y. K., K. K. Jung, K. Y. Chae, I. Jang, B. D. Lee and K. H. Nahm. 1995. Effects of vitamin E and seleium supplementation to diets containing aflatoxin B1 on the contents of liver lipids and various blood parameters in rats. Asian-Aust. J. Anim. Sci. 8(4):552-555.
  22. Machoy-Mokrzynska, A. 1995. Fluoride-magnesium interaction. Fluoride 28(3):175-177.

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